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Optical speckle patterns have been widely used for illumination in computational imaging, optical sectioning microscopy, and super-resolution imaging. However, commonly used speckles satisfy Rayleigh statistics, which are not ideal for…

Adaptive optics enables the deployment of interferometer-based spectroscopy without the need for moving parts necessary for scanning the interferometer arms. Here, we employ a Michelson Interferometer in conjunction with a Spatial Light…

Optics · Physics 2025-12-05 Jesneil Lauren Lewis , Ayan Banerjee

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

We demonstrate a new versatile method for 3D super-resolution microscopy by using a deformable mirror to shape the point spread function of our microscope in a continuous and controllable way. We apply this for 3D STORM imaging of…

Optics · Physics 2014-01-07 Nicolas Piro , Thomas Pengo , Nicolas Olivier , Suliana Manley

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

Face analysis has been studied from different angles to infer emotion, poses, shapes, and landmarks. Traditionally RGB cameras are used, yet for fine-grained tasks standard sensors might not be up to the task due to their latency, making it…

Computer Vision and Pattern Recognition · Computer Science 2024-10-30 Luca Cultrera , Federico Becattini , Lorenzo Berlincioni , Claudio Ferrari , Alberto Del Bimbo

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging…

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

In voltage imaging, where the membrane potentials of individual neurons are recorded at from hundreds to thousand frames per second using fluorescence microscopy, data processing presents a challenge. Even a fraction of a minute of…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yosuke Bando , Ramdas Pillai , Atsushi Kajita , Farhan Abdul Hakeem , Yves Quemener , Hua-an Tseng , Kiryl D. Piatkevich , Changyang Linghu , Xue Han , Edward S. Boyden

Accurate and fast 3D imaging of specular surfaces still poses major challenges for state-of-the-art optical measurement principles. Frequently used methods, such as phase-measuring deflectometry (PMD) or shape-from-polarization (SfP), rely…

Computer Vision and Pattern Recognition · Computer Science 2024-06-05 Jiazhang Wang , Oliver Cossairt , Florian Willomitzer

Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-01-03 Zhun Wei , Josiah R. Boivin , Yi Xue , Xudong Chen , Peter T. C. So , Elly Nedivi , Dushan N. Wadduwage

Our goal is to efficiently learn personalized animatable 3D head avatars from videos that are geometrically accurate, realistic, relightable, and compatible with current rendering systems. While 3D meshes enable efficient processing and are…

Computer Vision and Pattern Recognition · Computer Science 2023-10-30 Shrisha Bharadwaj , Yufeng Zheng , Otmar Hilliges , Michael J. Black , Victoria Fernandez-Abrevaya

Mapping blood microflows of the whole brain is crucial for early diagnosis of cerebral diseases. Ultrasound localization microscopy (ULM) was recently applied to map and quantify blood microflows in 2D in the brain of adult patients down to…

Medical Physics · Physics 2023-04-12 Hugues Favre , Mathieu Pernot , Mickael Tanter , Clément Papadacci

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…

Biological Physics · Physics 2011-12-23 Charles Edouard Leroux , Irène Wang , Jacques Derouard , Antoine Delon

Progress in neuroscience constantly relies on the development of new techniques to investigate the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally-invasive and high-resolution observations of neuronal…

In spite of being a valuable tool to simultaneously visualize multiple types of subcellular structures using spectrally distinct fluorescent labels, a standard fluoresce microscope is only able to identify a few microscopic objects; such a…

Image and Video Processing · Electrical Eng. & Systems 2025-02-25 Yang Jiao , Mei Yang , Mo Weng

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…

Quantitative Methods · Quantitative Biology 2019-08-15 Renata Rychtarikova , Georg Steiner , Gero Kramer , Michael B. Fischer , Dalibor Stys

Generating whole-brain 4D fMRI sequences conditioned on cognitive tasks remains challenging due to the high-dimensional, heterogeneous BOLD dynamics across subjects/acquisitions and the lack of neuroscience-grounded validation. We introduce…

Computer Vision and Pattern Recognition · Computer Science 2025-12-01 Jungwoo Seo , David Keetae Park , Shinjae Yoo , Jiook Cha

Thermal sensing with fine spatial resolution is important to the study of many scientific areas. While modern microscopy systems allow easy optical detection at high spatial resolution, their intrinsic functions are mainly focused on…

Optics · Physics 2018-07-04 Han Zhao , Zhaowei Chen , Ruogang Zhao , Liang Feng