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We introduce a novel gene regulatory network (GRN) inference method that integrates optimal transport (OT) with a deep-learning structural inference model. Advances in next-generation sequencing enable detailed yet destructive gene…

Computational Engineering, Finance, and Science · Computer Science 2024-09-24 Tsz Pan Tong , Aoran Wang , George Panagopoulos , Jun Pang

It is increasingly common to encounter data from dynamic processes captured by static cross-sectional measurements over time, particularly in biomedical settings. Recent attempts to model individual trajectories from this data use optimal…

Machine Learning · Statistics 2020-07-28 Alexander Tong , Jessie Huang , Guy Wolf , David van Dijk , Smita Krishnaswamy

Cell migration, which is strictly regulated by intracellular and extracellular cues, is crucial for normal physiological processes and the progression of certain diseases. However, there is a lack of an efficient approach to analyze…

Biological Physics · Physics 2025-04-11 Yanping Liu , Dui Qin , Xinwei Li , Guoqiang Li , Zhichao Liu , Kena Song , Wei Wang , Zhangyong Li

Rapid advance of experimental techniques provides an unprecedented in-depth view into complex developmental processes. Still, little is known on how the complexity of multicellular organisms evolved by elaborating developmental programs and…

Molecular Networks · Quantitative Biology 2021-07-16 Somya Mani , Tsvi Tlusty

Single-cell RNA-sequencing captures a temporal slice, or a snapshot, of a cell differentiation process. A major bioinformatical challenge is the inference of differentiation trajectories from a single snapshot, and methods that account for…

Quantitative Methods · Quantitative Biology 2025-02-11 Magnus Tronstad , Johan Karlsson , Joakim S. Dahlin

Single-cell RNA sequencing (scRNA-seq) technologies have enabled the profiling of gene expression for a collection of cells across time during a dynamic biological process. Given that each time point provides only a static snapshot,…

Applications · Statistics 2025-06-16 Binghao Yan , Hongzhe Li

Constructing cell developmental trajectories is a critical task in single-cell RNA sequencing (scRNA-seq) analysis, enabling the inference of potential cellular progression paths. However, current automated methods are limited to…

Computational Geometry · Computer Science 2025-07-31 Qipeng Wang , Shaolun Ruan , Rui Sheng , Yong Wang , Min Zhu , Huamin Qu

Single-cell RNA sequencing (scRNA-seq), especially temporally resolved datasets, enables genome-wide profiling of gene expression dynamics at single-cell resolution across discrete time points. However, current technologies provide only…

Genomics · Quantitative Biology 2025-11-19 Yue Ling , Peiqi Zhang , Zhenyi Zhang , Peijie Zhou

The rapid emergence of single-cell data has facilitated the study of many different biological conditions at the cellular level. Cluster analysis has been widely applied to identify cell types, capturing the essential patterns of the…

Genomics · Quantitative Biology 2025-02-03 Sebastian Pena , Lin Lin , Jia Li

Single and collective cell migration are fundamental processes critical for physiological phenomena ranging from embryonic development and immune response to wound healing and cancer metastasis. To understand cell migration from a physical…

Biological Physics · Physics 2023-10-10 David B. Brückner , Chase P. Broedersz

Single-particle tracking allows to infer the motion of single molecules in living cells. When we observe a long trajectory (more than 100 points), it is possible that the particle switches mode of motion over time. Then, fitting a single…

Methodology · Statistics 2018-04-16 Vincent Briane , Charles Kervrann , Myriam Vimond

Embryogenesis is a multiscale process during which developmental symmetry breaking transitions give rise to complex multicellular organisms. Recent advances in high-resolution live-cell microscopy provide unprecedented insights into the…

Biological Physics · Physics 2023-06-27 Nicolas Romeo , Alasdair Hastewell , Alexander Mietke , Jörn Dunkel

Our knowledge of how individual cells self-organize to form complex multicellular systems is being revolutionized by a data outburst, coming from high-throughput experimental breakthroughs such as single-cell RNA sequencing and spatially…

Cell Behavior · Quantitative Biology 2021-04-20 Gabriel Torregrosa , Jordi Garcia-Ojalvo

A general theoretical framework is put forth to organize and understand various observed phenomena and mathematical relationships in the field of molecular biology. By modeling each cell in eukaryotic organisms as a processor having a…

Other Quantitative Biology · Quantitative Biology 2013-12-18 Barry D. Jacobson

Trajectory inference is used to order single-cell omics data along a path that reflects a continuous transition between cells. This approach is useful for studying processes like cell differentiation, where a stem cell matures into a…

Quantitative Methods · Quantitative Biology 2025-12-23 Alexandre Hutton , Jesse G. Meyer

Multimodal single-cell technologies enable the simultaneous collection of diverse data types from individual cells, enhancing our understanding of cellular states. However, the integration of these datatypes and modeling the…

Machine Learning · Computer Science 2023-11-22 Bhavya Mehta , Nirmit Deliwala , Madhav Chandane

Cellular development follows a stochastic yet rule-governed trajectory, though the underlying principles remain elusive. Here, we propose that cellular development follows paths of least action, aligning with foundational physical laws that…

Biological Physics · Physics 2025-04-14 Rohola Zandie , Farhan Khodaee , Yufan Xia , Elazer R. Edelman

In this paper, we consider a tree inference problem motivated by the critical problem in single-cell genomics of reconstructing dynamic cellular processes from sequencing data. In particular, given a population of cells sampled from such a…

Methodology · Statistics 2025-07-16 Elodie Maignant , Tim Conrad , Christoph von Tycowicz

Single-cell reference atlases are large-scale, cell-level maps that capture cellular heterogeneity within an organ using single cell genomics. Given their size and cellular diversity, these atlases serve as high-quality training data for…

Genomics · Quantitative Biology 2022-11-09 Jan Engelmann , Leon Hetzel , Giovanni Palla , Lisa Sikkema , Malte Luecken , Fabian Theis
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