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While spatial transcriptomics (ST) has advanced our understanding of gene expression in tissue context, its high experimental cost limits its large-scale application. Predicting ST from pathology images is a promising, cost-effective…

Computer Vision and Pattern Recognition · Computer Science 2026-03-25 Zhiceng Shi , Changmiao Wang , Jun Wan , Wenwen Min

Spatial transcriptomics (ST) enables the visualization of gene expression within the context of tissue morphology. This emerging discipline has the potential to serve as a foundation for developing tools to design precision medicines.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-12 Shivam Kumar , Samrat Chatterjee

The rapid advancement of spatial transcriptomics (ST), i.e., spatial gene expressions, has made it possible to measure gene expression within original tissue, enabling us to discover molecular mechanisms. However, current ST platforms…

Computer Vision and Pattern Recognition · Computer Science 2025-11-11 Xiaofei Wang , Stephen Price , Chao Li

Spatial Transcriptomics (ST) is a method that captures gene expression profiles aligned with spatial coordinates. The discrete spatial distribution and the super-high dimensional sequencing results make ST data challenging to be modeled…

Machine Learning · Computer Science 2025-05-08 Qingtian Zhu , Yumin Zheng , Yuling Sang , Yifan Zhan , Ziyan Zhu , Jun Ding , Yinqiang Zheng

Spatial Transcriptomics (ST) offers spatially resolved gene expression but remains costly. Predicting expression directly from widely available Hematoxylin and Eosin (H&E) stained images presents a cost-effective alternative. However, most…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Jiarui Ouyang , Yihui Wang , Yihang Gao , Yingxue Xu , Shu Yang , Hao Chen

A comprehensive three-dimensional (3D) map of tissue architecture and gene expression is crucial for illuminating the complexity and heterogeneity of tissues across diverse biomedical applications. However, most spatial transcriptomics (ST)…

A recent technology breakthrough in spatial molecular profiling has enabled the comprehensive molecular characterizations of single cells while preserving spatial information. It provides new opportunities to delineate how cells from…

Applications · Statistics 2021-10-07 Xi Jiang , Qiwei Li , Guanghua Xiao

Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

This paper introduces new methodology to triangulate dynamic Bayesian networks (DBNs) and dynamic graphical models (DGMs). While most methods to triangulate such networks use some form of constrained elimination scheme based on properties…

Artificial Intelligence · Computer Science 2012-12-12 Jeff A. Bilmes , Chris Bartels

Spatial transcriptomics enables genome-wide expression analysis within native tissue context, yet identifying spatial domains remains challenging due to complex gene-spatial interactions. Existing methods typically process spatial and…

Machine Learning · Computer Science 2025-12-19 Jianping Mei , Siqi Ai , Ye Yuan

Spatial transcriptomics (ST) reveals spatial heterogeneity of gene expression, yet its resolution is limited by current platforms. Recent methods enhance resolution via H&E-stained histology, but three major challenges persist: (1)…

Image and Video Processing · Electrical Eng. & Systems 2025-08-12 Xuepeng Liu , Zheng Jiang , Pinan Zhu , Hanyu Liu , Chao Li

Spatially resolved transcriptomics is a fast-developing set of technologies that enables the measurement of localized gene expression across spatial locations in a sample. Detecting spatially varying genes is critical for analyzing such…

Applications · Statistics 2026-04-22 Pritam Dey , Rajarshi Guhaniyogi , Yang Ni , Bani K. Mallick

Single-cell RNA-sequencing technologies may provide valuable insights to the understanding of the composition of different cell types and their functions within a tissue. Recent technologies such as spatial transcriptomics, enable the…

Applications · Statistics 2023-05-16 Arhit Chakrabarti , Yang Ni , Bani K. Mallick

Spatial Transcriptomics enables mapping of gene expression within its native tissue context, but current platforms measure only a limited set of genes due to experimental constraints and excessive costs. To overcome this, computational…

Genomics · Quantitative Biology 2025-11-20 Amit Kumar , Maninder Kaur , Raghvendra Mall , Sukrit Gupta

Spatial transcriptomics (ST) technologies can be used to align transcriptomes with histopathological morphology, presenting exciting new opportunities for biomolecular discovery. Using ST data, we construct a novel framework, GeneFlow, to…

Quantitative Methods · Quantitative Biology 2025-11-04 Mengbo Wang , Shourya Verma , Aditya Malusare , Luopin Wang , Yiyang Lu , Vaneet Aggarwal , Mario Sola , Ananth Grama , Nadia Atallah Lanman

Spatially resolved transcriptomics represents a significant advancement in single-cell analysis by offering both gene expression data and their corresponding physical locations. However, this high degree of spatial resolution entails a…

Genomics · Quantitative Biology 2024-03-19 Xiaoyu Li , Wenwen Min , Shunfang Wang , Changmiao Wang , Taosheng Xu

Boundary information plays a significant role in 2D image segmentation, while usually being ignored in 3D point cloud segmentation where ambiguous features might be generated in feature extraction, leading to misclassification in the…

Computer Vision and Pattern Recognition · Computer Science 2021-01-08 Jingyu Gong , Jiachen Xu , Xin Tan , Jie Zhou , Yanyun Qu , Yuan Xie , Lizhuang Ma

Spatial variable genes (SVGs) reveal critical information about tissue architecture, cellular interactions, and disease microenvironments. As spatial transcriptomics (ST) technologies proliferate, accurately identifying SVGs across diverse…

Applications · Statistics 2025-10-21 Jiawen Chen , Jinwei Zhang , Dongshen Peng , Yutong Song , Aitong Ruan , Yun Li , Didong Li

In material science, image segmentation is of great significance for quantitative analysis of microstructures. Here, we propose a novel Weighted Propagation Convolution Neural Network based on U-Net (WPU-Net) to detect boundary in…

Computer Vision and Pattern Recognition · Computer Science 2025-07-11 Wei Liu , Jiahao Chen , Chuni Liu , Xiaojuan Ban , Boyuan Ma , Hao Wang , Weihua Xue , Yu Guo

Document binarization is a key pre-processing step for many document analysis tasks. However, existing methods can not extract stroke edges finely, mainly due to the fair-treatment nature of vanilla convolutions and the extraction of stroke…

Computer Vision and Pattern Recognition · Computer Science 2023-02-07 Zongyuan Yang , Yongping Xiong , Guibin Wu