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Large-scale cellular transformations are triggered by subtle physical and structural changes in individual biomacromolecular and membrane components. A prototypical example of such an event is the orchestrated fusion of membranes within an…

Soft Condensed Matter · Physics 2023-10-02 Ashesh Ghosh , Andrew J. Spkaowitz

Contraction assay based on surface measurement have been widely used to evaluate cell contractility in 3D models. This method is straightforward and requires no specific equipment, but it does not provide quantitative data about contraction…

We present feature finding and tracking algorithms in 3D in living cells, and demonstrate their utility to measure metrics important in cell biological processes. We developed a computational imaging hybrid approach that combines automated…

Biological Physics · Physics 2015-04-06 Kemp Plumb , Sarah Elaz , Vincent Pelletier , Maria L. Kilfoil

Compliant environments can mediate interactions between mechanically active cells like fibroblasts. Starting with a phenomenological model for the behaviour of single cells, we use extensive Monte Carlo simulations to predict non-trivial…

Soft Condensed Matter · Physics 2007-05-23 I. B. Bischofs , U. S. Schwarz

The optimal shapes attained by contractile cells on adhesive substrates are determined by the interplay between intracellular forces and adhesion with the extracellular matrix. We model the cell as a contractile film bounded by an elastic…

Biological Physics · Physics 2013-05-10 Shiladitya Banerjee , Luca Giomi

The effect of the anisotropy on the elastoplastic response of two dimensional packed samples of polygons is investigated here, using molecular dynamics simulation. We show a correlation between fabric coefficients, characterizing the…

Materials Science · Physics 2007-05-23 F. Alonso-Marroquin , S. Luding , H. J. Herrmann

During embryonic morphogenesis, tissues undergo dramatic deformations in order to form functional organs. Similarly, in adult animals, living cells and tissues are continually subjected to forces and deformations. Therefore, the success of…

Soft Condensed Matter · Physics 2024-05-06 Michael J. Hertaeg , Suzanne M. Fielding , Dapeng Bi

Fluid-structure interactions between active and passive components are important for many biological systems to function. A particular example is chromatin in the cell nucleus, where ATP-powered processes drive coherent motions of the…

Soft Condensed Matter · Physics 2024-02-15 Scott Weady , David B. Stein , Alexandra Zidovska , Michael J. Shelley

Activity in nematics drives interfacial flows that lead to preferential alignment that is tangential or planar for extensile systems (pushers) and perpendicular or homeotropic for contractile ones (pullers). This alignment is known as…

Soft Condensed Matter · Physics 2021-09-21 Rodrigo C. V. Coelho , Nuno A. M. Araújo , Margarida M. Telo da Gama

Active matter exhibits striking behaviour reminiscent of living matter and molecular fluids, and has promising applications in drug delivery or mixing at the micron scale. Active colloidal systems provide important models with simple and…

Soft Condensed Matter · Physics 2020-10-09 Nariaki Sakaï , C. Patrick Royall

In tissue engineering, 3D scaffolds and chemical treatments are often used for providing a cell-friendly surface for improving cell adhesion and tissue growth. Indeed, the cell adhesion degree can be controlled by physical-chemical changes…

Using Monte Carlo simulations of the Lebwohl--Lasher model we study the director ordering in a nematic cell where the top and bottom surfaces are patterned with a lattice of $\pm 1$ point topological defects of lattice spacing $a$. We find…

Soft Condensed Matter · Physics 2009-11-13 Adam S. Backer , A. C. Callan-Jones , Robert A. Pelcovits

Active processes drive and guide biological dynamics across scales -- from subcellular cytoskeletal remodelling, through tissue development in embryogenesis, to population-level bacterial colonies expansion. In each of these, biological…

Cells exhibit qualitatively different behaviors on substrates with different rigidities. The fact that cells are more polarized on the stiffer substrate motivates us to construct a two-dimensional cell with the distribution of focal…

Biological Physics · Physics 2017-07-26 Guangyuan Yu , Jingchen Feng , Haoran Man , Herbert Levine

Animal cells use traction forces to sense the mechanics and geometry of their environment. Measuring these traction forces requires a workflow combining cell experiments, image processing and force reconstruction based on elasticity theory.…

Quantitative Methods · Quantitative Biology 2016-08-24 Jerome R. Soine , Nils Hersch , Georg Dreissen , Nico Hampe , Bernd Hoffmann , Rudolf Merkel , Ulrich S. Schwarz

Active nematic models explain the topological defects and flow patterns observed in epithelial tissues, but the nature of active stress-whether it is extensile or contractile, a key parameter of the theory-is not well established…

Soft Condensed Matter · Physics 2025-11-11 Jan Rozman , Sumesh P. Thampi , Julia M. Yeomans

Biological tissues exhibit diverse mechanical and rheological behaviors during morphogenesis. While much is known about tissue phase transitions controlled by structural order and cell mechanics, key questions regarding how tissue-scale…

Soft Condensed Matter · Physics 2026-04-29 Pengyu Yu , Fridtjof Brauns , M. Cristina Marchetti

We numerically investigate the morphology and disclination line dynamics of active nematic droplets in three dimensions. Although our model only incorporates the simplest possible form of achiral active stress, active nematic droplets…

Soft Condensed Matter · Physics 2021-04-07 Liam J. Ruske , Julia M. Yeomans

The field of active nematics has traditionally employed descriptions based on dipolar activity, with interactions that align along a single axis. However, it has been theoretically predicted that interactions with a substrate, prevalent in…

Diffusion models are important in tissue engineering as they enable an understanding of molecular delivery to cells in tissue constructs. As three-dimensional (3D) tissue constructs become larger, more intricate, and more clinically…

Tissues and Organs · Quantitative Biology 2015-12-22 Richard J. McMurtrey