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We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Siyao Shao , Kevin Mallery , Santosh Kumar , Jiarong Hong

Particle size measurement based on digital holography with conventional algorithms are usually time-consuming and susceptible to noises associated with hologram quality and particle complexity, limiting its usage in a broad range of…

Applied Physics · Physics 2020-01-01 Siyao Shao , Kevin Mallery , Jiarong Hong

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…

Soft Condensed Matter · Physics 2020-02-25 Lauren E. Altman , David G. Grier

Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…

Fluid Dynamics · Physics 2024-12-25 Shyam Kumar M , Jiarong Hong

Aggregation-based assays, using micro- and nano-particles have been widely accepted as an efficient and cost-effective bio-sensing tool, particularly in microbiology, where particle clustering events are used as a metric to infer the…

Instrumentation and Detectors · Physics 2018-12-31 Yichen Wu , Aniruddha Ray , Qingshan Wei , Alborz Feizi , Xin Tong , Eva Chen , Yi Luo , Aydogan Ozcan

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

Colloidoscope is a deep learning pipeline employing a 3D residual Unet architecture, designed to enhance the tracking of dense colloidal suspensions through confocal microscopy. This methodology uses a simulated training dataset that…

Particle tracking is a fundamental task in digital microscopy. Recently, machine-learning approaches have made great strides in overcoming the limitations of more classical approaches. The training of state-of-the-art machine-learning…

Holography is an essential technique of generating three-dimensional images. Recently, quantum holography with undetected photons (QHUP) has emerged as a groundbreaking method capable of capturing complex amplitude images. Despite its…

We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…

Optics · Physics 2021-05-24 Hao Wang , Waleed Tahir , Jiabei Zhu , Lei Tian

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

Hyperspectral image analysis has become an important topic widely researched by the remote sensing community. Classification and segmentation of such imagery help understand the underlying materials within a scanned scene, since…

Computer Vision and Pattern Recognition · Computer Science 2020-12-02 Jakub Nalepa , Michal Myller , Yasuteru Imai , Ken-ichi Honda , Tomomi Takeda , Marek Antoniak

Holograms of colloidal particles can be analyzed with the Lorenz-Mie theory of light scattering to measure individual particles' three-dimensional positions with nanometer precision while simultaneously estimating their sizes and refractive…

Soft Condensed Matter · Physics 2018-07-04 Mark D. Hannel , Aidan Abdulali , Michael O'Brien , David G. Grier

Phase recovery from intensity-only measurements forms the heart of coherent imaging techniques and holography. Here we demonstrate that a neural network can learn to perform phase recovery and holographic image reconstruction after…

Computer Vision and Pattern Recognition · Computer Science 2017-12-13 Yair Rivenson , Yibo Zhang , Harun Gunaydin , Da Teng , Aydogan Ozcan

Emerging learned holography approaches have enabled faster and high-quality hologram synthesis, setting a new milestone toward practical holographic displays. However, these learned models require training a dedicated model for each set of…

Computer Vision and Pattern Recognition · Computer Science 2025-04-01 Yicheng Zhan , Liang Shi , Wojciech Matusik , Qi Sun , Kaan Akşit

Advanced microscopy and/or spectroscopy tools play indispensable role in nanoscience and nanotechnology research, as it provides rich information about the growth mechanism, chemical compositions, crystallography, and other important…

Digital holography is a 3D imaging technique by emitting a laser beam with a plane wavefront to an object and measuring the intensity of the diffracted waveform, called holograms. The object's 3D shape can be obtained by numerical analysis…

Information Retrieval · Computer Science 2022-07-14 Xiwen Chen , Hao Wang , Abolfazl Razi , Michael Kozicki , Christopher Mann

The classical method of determining the atomic structure of complex molecules by analyzing diffraction patterns is currently undergoing drastic developments. Modern techniques for producing extremely bright and coherent X-ray lasers allow a…

Biomolecules · Quantitative Biology 2015-10-12 Tomas Ekeberg , Stefan Engblom , Jing Liu

Reconstructing the 3D location and size of microparticles from diffraction images - holograms - is a computationally expensive inverse problem that has traditionally been solved using physics-based reconstruction methods. More recently,…

Computer Vision and Pattern Recognition · Computer Science 2025-03-17 Ayush Paliwal , Oliver Schlenczek , Birte Thiede , Manuel Santos Pereira , Katja Stieger , Eberhard Bodenschatz , Gholamhossein Bagheri , Alexander Ecker
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