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Obtaining in situ measurements of biological microparticles is crucial for both scientific research and numerous industrial applications (e.g., early detection of harmful algal blooms, monitoring yeast during fermentation). However,…
Microscopic analysis of micro particles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require…
We introduce the holographic air-quality monitor (HAM) system, uniquely tailored for monitoring large particulate matter (PM) over 10 um in diameter, i.e., particles critical for disease transmission and public health but overlooked by most…
Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…
The measurements of size distribution of small particles (e.g. dusts, droplets, bubbles, etc.) are critical for a broad range of applications in environmental science, public health, industrial manufacturing, etc. Laser diffraction (LD), a…
Current experiment techniques for vorticity measurement suffer from limited spatial and temporal resolution to resolve the small-scale eddy dynamics in turbulence. In this study, we develop a new method for direct vorticity measurement in…
The key limitations of digital inline holography (DIH) for particle tracking applications are poor longitudinal resolution, particle concentration limits, and case-specific processing. We utilize an inverse problem method with fused lasso…
Digital holography (DH) has been widely used for imaging and characterization of micro and nanostructures in materials science and biology and has the potential to provide high-resolution, non-destructive measurements of fluid surfaces as…
We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…
Accurate characterization of agricultural sprays is crucial to predict in field performance of liquid applied crop protection products. Here we introduce a robust and efficient machine learning (ML) based Digital In-line Holography (DIH) to…
Particle size measurement based on digital holography with conventional algorithms are usually time-consuming and susceptible to noises associated with hologram quality and particle complexity, limiting its usage in a broad range of…
Droplet generation through spray breakup is an unsteady and non-linear process which produces a relatively dense, highly polydisperse aerosol containing non-spherical droplets with sizes spanning several orders of magnitude. Such…
The coagulation of blood after it is drawn from the body poses a significant challenge for hematological analysis, potentially leading to inaccurate test results and altered cellular characteristics, compromising diagnostic reliability.…
Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…
Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…
Objective: Clinical implementation of deformable image registration (DIR) requires voxel-based spatial accuracy metrics such as manually identified landmarks, which are challenging to implement for highly mobile gastrointestinal (GI)…
This industrial Ph.D. project, carried out in collaboration between Radiometer Medical ApS and SDU Centre for Photonics Engineering at the University of Southern Denmark, explored the use of digital holographic microscopy (DHM) for the…
Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…
We have developed a lab work module where we teach undergraduate students how to quantify the dynamics of a suspension of microscopic particles, measuring and analyzing the motion of those particles at the individual level or as a group.…
De-identification of medical images is a critical step to ensure privacy during data sharing in research and clinical settings. The initial step in this process involves detecting Protected Health Information (PHI), which can be found in…