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Cell tracking is an essential tool in live-cell imaging to determine single-cell features, such as division patterns or elongation rates. Unlike in common multiple object tracking, in microbial live-cell experiments cells are growing,…

Computer Vision and Pattern Recognition · Computer Science 2023-03-20 Karina Ruzaeva , Jan-Christopher Cohrs , Keitaro Kasahara , Dietrich Kohlheyer , Katharina Nöh , Benjamin Berkels

Cell tracking is a key computational task in live-cell microscopy, but fully automated analysis of high-throughput imaging requires reliable and, thus, uncertainty-aware data analysis tools, as the amount of data recorded within a single…

Computer Vision and Pattern Recognition · Computer Science 2025-03-13 Richard D. Paul , Johannes Seiffarth , David Rügamer , Hanno Scharr , Katharina Nöh

Cell tracking and segmentation assist biologists in extracting insights from large-scale microscopy time-lapse data. Driven by local accuracy metrics, current tracking approaches often suffer from a lack of long-term consistency and the…

Computer Vision and Pattern Recognition · Computer Science 2025-06-30 Timo Kaiser , Maximilian Schier , Bodo Rosenhahn

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

Computer Vision and Pattern Recognition · Computer Science 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Live-cell imaging (LCI) technology enables the detailed spatio-temporal characterization of living cells at the single-cell level, which is critical for advancing research in the life sciences, from biomedical applications to bioprocessing.…

Computer Vision and Pattern Recognition · Computer Science 2025-10-09 Johannes Seiffarth , Keitaro Kasahara , Michelle Bund , Benita Lückel , Richard D. Paul , Matthias Pesch , Lennart Witting , Michael Bott , Dietrich Kohlheyer , Katharina Nöh

Cell tracking enables data extraction from time-lapse "cell movies" and promotes modeling biological processes at the single-cell level. We introduce a new fully automated computational strategy to track accurately cells across frames in…

Computer Vision and Pattern Recognition · Computer Science 2017-06-23 Athanasios D. Balomenos , Panagiotis Tsakanikas , Elias S. Manolakos

Cellular signaling is essential in information processing and decision making. Therefore, a variety of experimental approaches have been developed to study signaling on bulk and single-cell level. Single-cell measurements of signaling…

Quantitative Methods · Quantitative Biology 2019-04-18 Carolin Loos , Jan Hasenauer

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have…

Image and Video Processing · Electrical Eng. & Systems 2025-02-03 Gergely Szabó , Paolo Bonaiuti , Andrea Ciliberto , András Horváth

Analyzing the dynamic changes of cellular morphology is important for understanding the various functions and characteristics of live cells, including stem cells and metastatic cancer cells. To this end, we need to track all points on the…

Computer Vision and Pattern Recognition · Computer Science 2023-03-16 Junbong Jang , Kwonmoo Lee , Tae-Kyun Kim

Understanding the biological mechanisms of disease is crucial for medicine, and in particular, for drug discovery. AI-powered analysis of genome-scale biological data holds great potential in this regard. The increasing availability of…

Tracking all nuclei of an embryo in noisy and dense fluorescence microscopy data is a challenging task. We build upon a recent method for nuclei tracking that combines weakly-supervised learning from a small set of nuclei center point…

Computer Vision and Pattern Recognition · Computer Science 2022-08-25 Peter Hirsch , Caroline Malin-Mayor , Anthony Santella , Stephan Preibisch , Dagmar Kainmueller , Jan Funke

Single-molecule tracking is a powerful way to look at the dynamic organization of plasma membranes. However, there are some limitations to its use. For example, it was recently observed, using numerical simulation, that time-averaging…

Quantitative Methods · Quantitative Biology 2009-11-13 Nicolas Destainville , Laurence Salome

Determining the trajectories of cells and their lineages or ancestries in live-cell experiments are fundamental to the understanding of how cells behave and divide. This paper proposes novel online algorithms for jointly tracking and…

Signal Processing · Electrical Eng. & Systems 2021-10-29 Tran Thien Dat Nguyen , Ba-Ngu Vo , Ba-Tuong Vo , Du Yong Kim , Yu Suk Choi

In this work, we describe a method for large-scale 3D cell-tracking through a segmentation selection approach. The proposed method is effective at tracking cells across large microscopy datasets on two fronts: (i) It can solve problems…

Computer Vision and Pattern Recognition · Computer Science 2024-04-15 Jordão Bragantini , Merlin Lange , Loïc Royer

Cell tracking is a ubiquitous image analysis task in live-cell microscopy. Unlike multiple object tracking (MOT) for natural images, cell tracking typically involves hundreds of similar-looking objects that can divide in each frame, making…

Computer Vision and Pattern Recognition · Computer Science 2024-07-25 Benjamin Gallusser , Martin Weigert

We present Bionic Tracking, a novel method for solving biological cell tracking problems with eye tracking in virtual reality using commodity hardware. Using gaze data, and especially smooth pursuit eye movements, we are able to track cells…

Human-Computer Interaction · Computer Science 2020-08-04 Ulrik Günther , Kyle I. S. Harrington , Raimund Dachselt , Ivo F. Sbalzarini

In cell culture bioprocessing, real-time batch process monitoring (BPM) refers to the continuous tracking and analysis of key process variables such as viable cell density, nutrient levels, metabolite concentrations, and product titer…

Quantitative Methods · Quantitative Biology 2025-12-04 Johnny Peng , Thanh Tung Khuat , Ellen Otte , Katarzyna Musial , Bogdan Gabrys

The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using…

Computer Vision and Pattern Recognition · Computer Science 2022-08-03 Andrew Lauziere , Ryan Christensen , Hari Shroff
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