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Single-molecule fluorescence techniques provide exceptional sensitivity to probe biomolecular interactions. However, their application to accurately quantify analytes at the picomolar concentrations relevant for biosensing remains…

Optics · Physics 2025-02-18 Malavika Kayyil Veedu , Jérôme Wenger

Image/video denoising in low-light scenes is an extremely challenging problem due to limited photon count and high noise. In this paper, we propose a novel approach with contrastive learning to address this issue. Inspired by the success of…

Computer Vision and Pattern Recognition · Computer Science 2023-05-08 Taoyong Cui , Yuhan Dong

In low-light conditions, capturing videos with frame-based cameras often requires long exposure times, resulting in motion blur and reduced visibility. While frame-based motion deblurring and low-light enhancement have been studied, they…

Computer Vision and Pattern Recognition · Computer Science 2024-08-28 Taewoo Kim , Jaeseok Jeong , Hoonhee Cho , Yuhwan Jeong , Kuk-Jin Yoon

Real-world imaging systems acquire measurements that are degraded by noise, optical aberrations, and other imperfections that make image processing for human viewing and higher-level perception tasks challenging. Conventional cameras…

Computer Vision and Pattern Recognition · Computer Science 2021-05-11 Steven Diamond , Vincent Sitzmann , Frank Julca-Aguilar , Stephen Boyd , Gordon Wetzstein , Felix Heide

Low-light imaging is challenging since images may appear to be dark and noised due to low signal-to-noise ratio, complex image content, and the variety in shooting scenes in extreme low-light condition. Many methods have been proposed to…

Image and Video Processing · Electrical Eng. & Systems 2020-05-19 Keqi Wang , Peng Gao , Steven Hoi , Qian Guo , Yuhua Qian

Recent work indicates that, besides being a challenge in producing perceptually pleasing images, low light proves more difficult for machine cognition than previously thought. In our work, we take a closer look at object detection in low…

Computer Vision and Pattern Recognition · Computer Science 2021-10-22 Igor Morawski , Yu-An Chen , Yu-Sheng Lin , Winston H. Hsu

Scene understanding under low-light conditions is a challenging problem. This is due to the small number of photons captured by the camera and the resulting low signal-to-noise ratio (SNR). Single-photon cameras (SPCs) are an emerging…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Bhavya Goyal , Mohit Gupta

Fiber photometry permits monitoring fluorescent indicators of neural activity in behaving animals. Optical fibers are typically used to excite and collect fluorescence from genetically-encoded calcium indicators expressed by a subset of…

I propose a superoscillation measurement method for subdiffraction incoherent optical sources, with potential applications in astronomy, remote sensing, fluorescence microscopy, and spectroscopy. The proposal, based on coherent optical…

Quantum Physics · Physics 2022-10-10 Mankei Tsang

A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently,…

Low-light is an inescapable element of our daily surroundings that greatly affects the efficiency of our vision. Research works on low-light has seen a steady growth, particularly in the field of image enhancement, but there is still a lack…

Computer Vision and Pattern Recognition · Computer Science 2018-05-30 Yuen Peng Loh , Chee Seng Chan

Fluorescence microscopy, while being a key driver for progress in the life sciences, is also subject to technical limitations. To overcome them, computational multiplexing techniques have recently been proposed, which allow multiple…

Computer Vision and Pattern Recognition · Computer Science 2025-10-22 Ashesh Ashesh , Florian Jug

MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics,…

Computational Physics · Physics 2026-04-17 Steffen Schultze , Helmut Grubmüller

Imaging in low light is difficult because the number of photons arriving at the sensor is low. Imaging dynamic scenes in low-light environments is even more difficult because as the scene moves, pixels in adjacent frames need to be aligned…

Image and Video Processing · Electrical Eng. & Systems 2020-07-20 Yiheng Chi , Abhiram Gnanasambandam , Vladlen Koltun , Stanley H. Chan

Saliency detection methods are central to several real-world applications such as robot navigation and satellite imagery. However, the performance of existing methods deteriorate under low-light conditions because training datasets mostly…

Computer Vision and Pattern Recognition · Computer Science 2023-03-20 Kitty Varghese , Sudarshan Rajagopalan , Mohit Lamba , Kaushik Mitra

Capturing images under extremely low-light conditions poses significant challenges for the standard camera pipeline. Images become too dark and too noisy, which makes traditional image enhancement techniques almost impossible to apply. Very…

Computer Vision and Pattern Recognition · Computer Science 2020-06-19 Ahmet Serdar Karadeniz , Erkut Erdem , Aykut Erdem

As vision based perception methods are usually built on the normal light assumption, there will be a serious safety issue when deploying them into low light environments. Recently, deep learning based methods have been proposed to enhance…

Computer Vision and Pattern Recognition · Computer Science 2020-10-21 Junjie Hu , Xiyue Guo , Junfeng Chen , Guanqi Liang , Fuqin Deng , Tin lun Lam

Fluorescence microscopy has enabled a dramatic development in modern biology. Due to its inherently weak signal, fluorescence microscopy is not only much noisier than photography, but also presented with Poisson-Gaussian noise where Poisson…

Computer Vision and Pattern Recognition · Computer Science 2019-04-09 Yide Zhang , Yinhao Zhu , Evan Nichols , Qingfei Wang , Siyuan Zhang , Cody Smith , Scott Howard

Scene reconstruction in the presence of high-speed motion and low illumination is important in many applications such as augmented and virtual reality, drone navigation, and autonomous robotics. Traditional motion estimation techniques fail…

Computer Vision and Pattern Recognition · Computer Science 2023-09-08 Sacha Jungerman , Atul Ingle , Mohit Gupta

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman