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Single-cell RNA sequencing (scRNA-seq) is essential for decoding tumor heterogeneity. However, pan-cancer research still faces two key challenges: learning discriminative and efficient single-cell representations, and establishing a…

Genomics · Quantitative Biology 2025-12-04 Xiaoshui Huang , Tianlin Zhu , Yifan Zuo , Xue Xia , Zonghan Wu , Jiebin Yan , Dingli Hua , Zongyi Xu , Yuming Fang , Jian Zhang

We introduce a unified framework for evaluating dimensionality reduction techniques in spatial transcriptomics beyond standard PCA approaches. We benchmark six methods PCA, NMF, autoencoder, VAE, and two hybrid embeddings on a…

Single-nucleus RNA sequencing (snRNA-seq) has significantly advanced our understanding of the disease etiology of neurodegenerative disorders. However, the low quality of specimens derived from postmortem brain tissues, combined with the…

Genomics · Quantitative Biology 2025-02-28 Gyutaek Oh , Baekgyu Choi , Seyoung Jin , Inkyung Jung , Jong Chul Ye

Principal component analysis (PCA) is a standard tool for dimensional reduction of a set of $n$ observations (samples), each with $p$ variables. In this paper, using a matrix perturbation approach, we study the nonasymptotic relation…

Statistics Theory · Mathematics 2009-01-22 Boaz Nadler

Identity, response to external stimuli, and spatial architecture of a living system are central topics of molecular biology. Presently, they are largely seen as a result of the interplay between a gene repertoire and the regulatory…

Molecular Networks · Quantitative Biology 2007-05-23 M. Cosentino Lagomarsino , P. Jona , B. Bassetti

Many modern biological assays, including RNA sequencing, yield integer-valued counts that reflect the number of molecules detected. These measurements are often not at the desired resolution: while the unit of interest is typically a single…

Machine Learning · Computer Science 2026-03-06 Nic Fishman , Gokul Gowri , Tanush Kumar , Jiaqi Lu , Valentin de Bortoli , Jonathan S. Gootenberg , Omar Abudayyeh

Learning causal relationships from time series data is an important but challenging problem. Existing synthetic datasets often contain hidden artifacts that can be exploited by causal discovery methods, reducing their usefulness for…

Machine Learning · Computer Science 2026-03-23 Xiaoyu He , Petr Ryšavý , Jakub Mareček

Accurate segmentation of carotid artery structures in histopathological images is vital for cardiovascular disease research. This study systematically evaluates ten deep learning segmentation models including classical architectures, modern…

Computer Vision and Pattern Recognition · Computer Science 2026-04-07 Phongsakon Mark Konrad , Andrei-Alexandru Popa , Yaser Sabzehmeidani , Liang Zhong , Madhulika Tripathy , Andrei Constantinescu , Elisa A. Liehn , Serkan Ayvaz

Modern high-throughput gene perturbation screens are key technologies at the forefront of genetic research. Combined with rich phenotypic descriptors they enable researchers to observe detailed cellular reactions to experimental…

Molecular Networks · Quantitative Biology 2010-10-08 Florian Markowetz

A transversal topic of my research has been the development and application of computational methods for DNA sequence analysis. The methods I have been developing aim at improving our understanding of the regulation processes happening in…

Genomics · Quantitative Biology 2014-09-24 Valentina Boeva

Advances in single-cell omics allow for unprecedented insights into the transcription profiles of individual cells. When combined with large-scale perturbation screens, through which specific biological mechanisms can be targeted, these…

Machine Learning · Computer Science 2023-10-24 Alejandro Tejada-Lapuerta , Paul Bertin , Stefan Bauer , Hananeh Aliee , Yoshua Bengio , Fabian J. Theis

Genomic signal processing has been used successfully in bioinformatics to analyze biomolecular sequences and gain varied insights into DNA structure, gene organization, protein binding, sequence evolution, etc. But challenges remain in…

Genomics · Quantitative Biology 2022-11-04 Saish Jaiswal , Shreya Nema , Hema A Murthy , Manikandan Narayanan

Over the years, Principal Component Analysis (PCA) has served as the baseline approach for dimensionality reduction in gene expression data analysis. It primary objective is to identify a subset of disease-causing genes from a vast pool of…

Algebraic Topology · Mathematics 2023-06-13 Sean Cottrell , Rui Wang , Guowei Wei

The network paradigm is increasingly used to describe the dynamics of complex systems. Here we review the current results and propose future development areas in the assessment of perturbation waves, i.e. propagating structural changes in…

Molecular Networks · Quantitative Biology 2009-04-23 Miklos A. Antal , Csaba Bode , Peter Csermely

A theory for qualitative models of gene regulatory networks has been developed over several decades, generally considering transcription factors to regulate directly the expression of other transcription factors, without any intermediate…

Classical Analysis and ODEs · Mathematics 2016-07-20 D. Hudson , R. Edwards

To understand neural network behavior, recent works quantitatively compare different networks' learned representations using canonical correlation analysis (CCA), centered kernel alignment (CKA), and other dissimilarity measures.…

Machine Learning · Computer Science 2021-11-04 Frances Ding , Jean-Stanislas Denain , Jacob Steinhardt

Predicting how genetic perturbations change cellular state is a core problem for building controllable models of gene regulation. Perturbations targeting the same gene can produce different transcriptional responses depending on their…

Genomics · Quantitative Biology 2026-02-12 Boyang Fu , George Dasoulas , Sameer Gabbita , Xiang Lin , Shanghua Gao , Xiaorui Su , Soumya Ghosh , Marinka Zitnik

Reconstructing transcriptional regulatory networks is an important task in functional genomics. Data obtained from experiments that perturb genes by knockouts or RNA interference contain useful information for addressing this reconstruction…

Machine Learning · Statistics 2015-06-18 Ali Shojaie , Alexandra Jauhiainen , Michael Kallitsis , George Michailidis

Atomic-level simulations are widely used to study biomolecules and their dynamics. A common goal in such studies is to compare simulations of a molecular system under several conditions -- for example, with various mutations or bound…

Biomolecules · Quantitative Biology 2025-01-07 Martin Vögele , Neil J. Thomson , Sang T. Truong , Jasper McAvity , Ulrich Zachariae , Ron O. Dror

Comparative transcriptomics has gained increasing popularity in genomic research thanks to the development of high-throughput technologies including microarray and next-generation RNA sequencing that have generated numerous transcriptomic…

Quantitative Methods · Quantitative Biology 2021-12-01 Wei Vivian Li , Yiling Chen , Jingyi Jessica Li
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