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Spatial transcriptomics (ST) provides spatially resolved measurements of gene expression, enabling characterization of the molecular landscape of human tissue beyond histological assessment as well as localized readouts that can be aligned…

Computer Vision and Pattern Recognition · Computer Science 2026-02-17 Konstantin Hemker , Andrew H. Song , Cristina Almagro-Pérez , Guillaume Jaume , Sophia J. Wagner , Anurag Vaidya , Nikola Simidjievski , Mateja Jamnik , Faisal Mahmood

Quantitative characterization of cellular spatial organization is critical for understanding tumor progression and immune response. Recent advances in artificial intelligence (AI) enable large-scale segmentation and classification of nuclei…

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

Spatial transcriptomics provides an unprecedented perspective for deciphering tissue spatial heterogeneity. However, high-resolution spatial transcriptomic technology remains constrained by limited gene coverage, technical complexity, and…

Biomolecules · Quantitative Biology 2026-05-19 Xinlei Huang , Weihao Dai , Zijun Qin , Xin Yu , Di Wang , Yanran Liu , Lixin Cheng , Xubin Zheng

Spatial transcriptomics (ST) profiles gene expression across a tissue section while preserving the spatial coordinates. Because current ST technologies typically profile two-dimensional tissue slices, integrating and aligning slices from…

Quantitative Methods · Quantitative Biology 2026-03-20 Yaqi Wu , Jingfeng Wang , Xin Maizie Zhou , Yanxiang Zhao , Zixuan Cang

Spatial transcriptomics measures the expression of thousands of genes in a tissue sample while preserving its spatial structure. This class of technologies has enabled the investigation of the spatial variation of gene expressions and their…

Methodology · Statistics 2025-10-23 Andrea Sottosanti , Davide Risso , Francesco Denti

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min

In this paper, we introduce QuST-LLM, an innovative extension of QuPath that utilizes the capabilities of large language models (LLMs) to analyze and interpret spatial transcriptomics (ST) data. In addition to simplifying the intricate and…

Genomics · Quantitative Biology 2024-07-03 Chao Hui Huang

The integration of AI in digital pathology, particularly in whole slide image (WSI) and spatial transcriptomics (ST) analysis, holds immense potential for enhancing our understanding of diseases. Despite challenges such as training pattern…

Quantitative Methods · Quantitative Biology 2024-11-18 Chao-Hui Huang , Sara Lichtarge , Diane Fernandez

While pathology foundation models have transformed cancer image analysis, they often lack integration with molecular data at single-cell resolution, limiting their utility for precision oncology. Here, we present PAST, a pan-cancer…

Quantitative Methods · Quantitative Biology 2025-07-10 Changchun Yang , Haoyang Li , Yushuai Wu , Yilan Zhang , Yifeng Jiao , Yu Zhang , Rihan Huang , Yuan Cheng , Yuan Qi , Xin Guo , Xin Gao

Human and animal tissues consist of heterogeneous cell types that organize and interact in highly structured manners. Bulk and single-cell sequencing technologies remove cells from their original microenvironments, resulting in a loss of…

Quantitative Methods · Quantitative Biology 2022-02-08 Boxiang Liu , Yanjun Li , Liang Zhang

Spatial transcriptomics enables gene expression profiling with spatial context, offering unprecedented insights into the tissue microenvironment. However, most computational models treat genes as isolated numerical features, ignoring the…

Machine Learning · Computer Science 2025-11-17 Jiangkai Long , Yanran Zhu , Chang Tang , Kun Sun , Yuanyuan Liu , Xuesong Yan

Xenium, a new spatial transcriptomics platform, enables subcellular-resolution profiling of complex tumor tissues. Despite the rich morphological information in histology images, extracting robust cell-level features and integrating them…

Computer Vision and Pattern Recognition · Computer Science 2025-08-15 Paul H. Acosta , Pingjun Chen , Simon P. Castillo , Maria Esther Salvatierra , Yinyin Yuan , Xiaoxi Pan

Nuclei instance segmentation in histopathological images is of great importance for biological analysis and cancer diagnosis but remains challenging for two reasons. (1) Similar visual presentation of intranuclear and extranuclear regions…

Computer Vision and Pattern Recognition · Computer Science 2024-02-12 Ye Zhang , Linghan Cai , Ziyue Wang , Yongbing Zhang

Motivation: A major challenge in metabolomics is annotation: assigning molecular structures to mass spectral fragmentation patterns. Despite recent advances in molecule-to-spectra and in spectra-to-molecular fingerprint prediction (FP),…

Quantitative Methods · Quantitative Biology 2025-06-10 Apurva Kalia , Yan Zhou Chen , Dilip Krishnan , Soha Hassoun

Spatial transcriptomics (ST) has revolutionised transcriptomics analysis by preserving tissue architecture, allowing researchers to study gene expression in its native spatial context. However, despite its potential, ST still faces…

Quantitative Methods · Quantitative Biology 2025-05-19 Anthony Baptista , Rosamond Nuamah , Ciro Chiappini , Anita Grigoriadis

The advancement of mobile computing devices and positioning technologies has led to an explosive growth of spatio-temporal data managed in databases. Representative queries over such data include range queries, nearest neighbor queries, and…

Databases · Computer Science 2026-01-23 Xieyang Wang , Mengyi Liu , Weijia Yi , Jianqiu Xu , Raymond Chi-Wing Wong

Spatial Transcriptomics (ST) reveals the spatial distribution of gene expression in tissues, offering critical insights into biological processes and disease mechanisms. However, the high cost, limited coverage, and technical complexity of…

Computer Vision and Pattern Recognition · Computer Science 2025-04-22 Yi Niu , Jiashuai Liu , Yingkang Zhan , Jiangbo Shi , Di Zhang , Marika Reinius , Ines Machado , Mireia Crispin-Ortuzar , Jialun Wu , Chen Li , Zeyu Gao

As a fundamental part of computational healthcare, Computer Tomography (CT) and Magnetic Resonance Imaging (MRI) provide volumetric data, making the development of algorithms for 3D image analysis a necessity. Despite being computationally…

Image and Video Processing · Electrical Eng. & Systems 2023-07-26 C. I. Ugwu , S. Casarin , O. Lanz

Spatial transcriptomics (ST) is a promising technique that characterizes the spatial gene profiling patterns within the tissue context. Comprehensive ST analysis depends on consecutive slices for 3D spatial insights, whereas the missing…

Image and Video Processing · Electrical Eng. & Systems 2025-05-19 NingFeng Que , Xiaofei Wang , Jingjing Chen , Yixuan Jiang , Chao Li