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Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…

Lasers that combine narrow linewidths with rapid tunability are critical for applications such as coherent optical ranging, distributed fiber-optic sensing, and precision spectroscopy. Despite significant progress in integrated laser…

Infrared (IR) imaging is invaluable across many scientific disciplines, from material analysis to diagnostic medicine. However, applications are often limited by detector cost, resolution and sensitivity, noise caused by the thermal IR…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek

Solving optimization problems is challenging for existing digital computers and even for future quantum hardware. The practical importance of diverse problems, from healthcare to financial optimization, has driven the emergence of…

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Our proposed method of random phase-free holography using virtual convergence light can obtain large reconstructed images exceeding the size of the hologram, without the assistance of random phase. The reconstructed images have low-speckle…

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…

Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…

Optics · Physics 2025-09-09 Shi Zhao , Haowen Zhou , Changhuei Yang

Image Quality Assessment (IQA) algorithms evaluate the perceptual quality of an image using evaluation scores that assess the similarity or difference between two images. We propose a new low-level feature based IQA technique, which applies…

Multimedia · Computer Science 2017-12-04 Navaneeth K. Kottayil , Irene Cheng , Frederic Dufaux , Anup Basu

Quantitative phase imaging (QPI) is a label-free computational imaging technique that provides optical path length information of specimens. In modern implementations, the quantitative phase image of an object is reconstructed digitally…

Optics · Physics 2022-05-23 Deniz Mengu , Aydogan Ozcan

This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront…

Second harmonic generation (SHG) microscopy is a valuable tool for optical microscopy. SHG microscopy is normally performed as a point scanning imaging method, which lacks phase information and is limited in spatial resolution by the…

In this work, we present a highly efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination based on traditional bright-field microscope. As a new type of coded illumination, the LED array provides a…

Optics · Physics 2017-07-14 Jiaji Li , Qian Chen , Jialin Zhang , Yan Zhang , Linpeng Lu , Chao Zuo

We propose a holographic microscopy method based on single-pixel imaging technology (HM-SPI). We used a holographic microscopy method based on in-line Gabor holography. In single-pixel imaging technology, cyclic binary masks and…

Optics · Physics 2025-04-11 Arthur G. Fedorov

For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent…

Optics · Physics 2017-05-31 Chao Zuo , Jiasong Sun , Jiaji Li , Jialin Zhang , Anand Asundi , Qian Chen

This study introduces a Holographic Agglutination Assay for quantifying levels of the immunoglobulin protein IgA in biological samples. This is the first example of a label-free and bead-free assay that quantifies protein agglutinates by…

Optics · Physics 2025-10-16 Rushna Quddus , Kent Kirshenbaum , David G. Grier

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

Optics · Physics 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun

We propose a quantum-assisted reconstruction framework for high-resolution tomographic imaging that significantly reduces both qubit requirements and radiation exposure. Conventional quantum reconstruction methods require solving QUBO…

Quantum Physics · Physics 2025-04-30 Hyunju Lee , Kyungtaek Jun