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The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Spatially resolved transcriptomics brings exciting breakthroughs to single-cell analysis by providing physical locations along with gene expression. However, as a cost of the extremely high spatial resolution, the cellular level spatial…

Genomics · Quantitative Biology 2024-02-19 Hongzhi Wen , Wenzhuo Tang , Wei Jin , Jiayuan Ding , Renming Liu , Xinnan Dai , Feng Shi , Lulu Shang , Hui Liu , Yuying Xie

Recent advancements in spatial transcriptomics technologies allow researchers to simultaneously measure RNA expression levels for hundreds to thousands of genes while preserving spatial information within tissues, providing critical…

Methodology · Statistics 2025-07-31 Catherine Higgins , Jingyi Jessica Li , Michelle Carey

We propose a new regularized optimal transport (OT) formulation, termed sliced-regularized optimal transport (SROT). Unlike entropic OT (EOT), which regularizes the transport plan toward an independent coupling, SROT regularizes it toward a…

Machine Learning · Statistics 2026-05-21 Khai Nguyen

Single-cell RNA-sequencing captures a temporal slice, or a snapshot, of a cell differentiation process. A major bioinformatical challenge is the inference of differentiation trajectories from a single snapshot, and methods that account for…

Quantitative Methods · Quantitative Biology 2025-02-11 Magnus Tronstad , Johan Karlsson , Joakim S. Dahlin

Efficient comparison of spherical probability distributions becomes important in fields such as computer vision, geosciences, and medicine. Sliced optimal transport distances, such as spherical and stereographic spherical sliced Wasserstein…

Recent advancements in Spatial Transcriptomics (ST) technology have facilitated detailed gene expression analysis within tissue contexts. However, the high costs and methodological limitations of ST necessitate a more robust predictive…

Computer Vision and Pattern Recognition · Computer Science 2024-04-26 Youngmin Chung , Ji Hun Ha , Kyeong Chan Im , Joo Sang Lee

Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

We demonstrate the effectiveness of one of the many multi-tracer analyses enabled by Optimal Transport (OT) reconstruction. Leveraging a semi-discrete OT algorithm, we determine the displacements between initial and observed positions of…

Cosmology and Nongalactic Astrophysics · Physics 2024-03-19 Farnik Nikakhtar , Ravi K. Sheth , Nikhil Padmanabhan , Bruno Lévy , Roya Mohayaee

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

Histopathology is critical for the diagnosis of many diseases, including cancer. These protocols typically require pathologists to manually evaluate slides under a microscope, which is time-consuming and subjective, leading to interest in…

Computer Vision and Pattern Recognition · Computer Science 2023-03-07 Kianoush Falahkheirkhah , Alex Lu , David Alvarez-Melis , Grace Huynh

Spatial transcriptomics provides an unprecedented perspective for deciphering tissue spatial heterogeneity. However, high-resolution spatial transcriptomic technology remains constrained by limited gene coverage, technical complexity, and…

Biomolecules · Quantitative Biology 2026-05-19 Xinlei Huang , Weihao Dai , Zijun Qin , Xin Yu , Di Wang , Yanran Liu , Lixin Cheng , Xubin Zheng

Discriminating between distributions is an important problem in a number of scientific fields. This motivated the introduction of Linear Optimal Transportation (LOT), which embeds the space of distributions into an $L^2$-space. The…

Machine Learning · Statistics 2021-05-27 Caroline Moosmüller , Alexander Cloninger

Spatial Transcriptomics enables mapping of gene expression within its native tissue context, but current platforms measure only a limited set of genes due to experimental constraints and excessive costs. To overcome this, computational…

Genomics · Quantitative Biology 2025-11-20 Amit Kumar , Maninder Kaur , Raghvendra Mall , Sukrit Gupta

Self-supervised learning (SSL) has emerged as a promising technique for analyzing medical modalities such as X-rays due to its ability to learn without annotations. However, conventional SSL methods face challenges in achieving semantic…

Computer Vision and Pattern Recognition · Computer Science 2025-03-12 Vandan Gorade , Azad Singh , Deepak Mishra

Standard representational similarity methods align each layer of a network to its best match in another independently, producing asymmetric results, lacking a global alignment score, and struggling with networks of different depths. These…

Machine Learning · Computer Science 2026-04-23 Shaan Shah , Meenakshi Khosla

Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Symbolic regression (SR) aims to discover mathematical expressions from data, a task traditionally tackled using Genetic Programming (GP) through combinatorial search over symbolic structures. Latent Space Optimization (LSO) methods use…

Neural and Evolutionary Computing · Computer Science 2026-04-14 Benjamin Léger , Kazem Meidani , Christian Gagné

Spatial Transcriptomics is a groundbreaking technology that integrates histology images with spatially resolved gene expression profiles. Among the various Spatial Transcriptomics techniques available, Visium has emerged as the most widely…

Computer Vision and Pattern Recognition · Computer Science 2025-09-05 Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Gabriel M. Mejia , Pablo Arbeláez

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min