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The current study detects different morphologies related to prostate pathology using deep learning models; these models were evaluated on 2,121 hematoxylin and eosin (H&E) stain histology images captured using bright field microscopy, which…

Understanding the way cells communicate, co-locate, and interrelate is essential to furthering our understanding of how the body functions. H&E is widely available, however, cell subtyping often requires expert knowledge and the use of…

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

Early diagnosis of the cancer cells is necessary for making an effective treatment plan and for the health and safety of a patient. Nowadays, doctors usually use a histological grade that pathologists determine by performing a…

Computer Vision and Pattern Recognition · Computer Science 2023-11-15 Varan Singh Rohila , Neeraj Lalwani , Lochan Basyal

The overexpression of the human epidermal growth factor receptor 2 (HER2) in breast cells is a key driver of HER2-positive breast cancer, a highly aggressive subtype requiring precise diagnosis and targeted therapy. Immunohistochemistry…

Image and Video Processing · Electrical Eng. & Systems 2026-03-10 Sara Rehmat , Hafeez Ur Rehman , Byeong-Gwon Kang , Sarra Ayouni , Yunyoung Nam

Early detection of lung cancer is critical for improvement of patient survival. To address the clinical need for efficacious treatments, genetically engineered mouse models (GEMM) have become integral in identifying and evaluating the…

We introduce a new AI-ready computational pathology dataset containing restained and co-registered digitized images from eight head-and-neck squamous cell carcinoma patients. Specifically, the same tumor sections were stained with the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-29 Parmida Ghahremani , Joseph Marino , Juan Hernandez-Prera , Janis V. de la Iglesia , Robbert JC Slebos , Christine H. Chung , Saad Nadeem

The effective management of brain tumors relies on precise typing, subtyping, and grading. This study advances patient care with findings from rigorous multiple instance learning experimentations across various feature extractors and…

Computer Vision and Pattern Recognition · Computer Science 2024-03-11 Ekansh Chauhan , Amit Sharma , Megha S Uppin , C. V. Jawahar , P. K. Vinod

Thyroid cancer is the most common endocrine malignancy, and accurately distinguishing between benign and malignant thyroid tumors is crucial for developing effective treatment plans in clinical practice. Pathologically, thyroid tumors pose…

Image and Video Processing · Electrical Eng. & Systems 2024-01-04 Jia Dong , Yao Yao , Yang Dong , Hui Ma

Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

The large volume and variety of imaging data from high-throughput screening (HTS) in the pharmaceutical industry present an excellent resource for training virtual staining models. However, the potential of models trained under one set of…

Machine Learning · Computer Science 2024-10-01 Samuel Tonks , Cuong Nguyen , Steve Hood , Ryan Musso , Ceridwen Hopely , Steve Titus , Minh Doan , Iain Styles , Alexander Krull

We propose a Deep learning-based weak label learning method for analyzing whole slide images (WSIs) of Hematoxylin and Eosin (H&E) stained tumor tissue not requiring pixel-level or tile-level annotations using Self-supervised pre-training…

Image and Video Processing · Electrical Eng. & Systems 2023-06-29 Yoni Schirris , Efstratios Gavves , Iris Nederlof , Hugo Mark Horlings , Jonas Teuwen

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

Quantitative Methods · Quantitative Biology 2026-05-19 Yucheng Xing , Pei Liu , Jingying Ma , Ruping Hong , Jiangdong Qiu , Tianyu Liu , Kai He , Ling Huang , Mengling Feng

The differentiation between pathological subtypes of non-small cell lung cancer (NSCLC) is an essential step in guiding treatment options and prognosis. However, current clinical practice relies on multi-step staining and labelling…

Quantitative Methods · Quantitative Biology 2026-03-10 Zhenya Zang , David A Dorward , Katherine E Quiohilag , Andrew DJ Wood , James R Hopgood , Ahsan R Akram , Qiang Wang

Predicting the response of a patient to a cancer treatment is of high interest. Nonetheless, this task is still challenging from a medical point of view due to the complexity of the interaction between the patient organism and the…

Computer Vision and Pattern Recognition · Computer Science 2024-08-09 Bilel Guetarni , Feryal Windal , Halim Benhabiles , Mahfoud Chaibi , Romain Dubois , Emmanuelle Leteurtre , Dominique Collard

Breast Cancer is a major cause of death worldwide among women. Hematoxylin and Eosin (H&E) stained breast tissue samples from biopsies are observed under microscopes for the primary diagnosis of breast cancer. In this paper, we propose a…

Computer Vision and Pattern Recognition · Computer Science 2018-07-26 Aditya Golatkar , Deepak Anand , Amit Sethi

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Due to the recent advancements in machine vision, digital pathology has gained significant attention. Histopathology images are distinctly rich in visual information. The tissue glass slide images are utilized for disease diagnosis.…

Image and Video Processing · Electrical Eng. & Systems 2020-11-02 Danial Maleki , Mehdi Afshari , Morteza Babaie , H. R. Tizhoosh

Histological images are obtained by transmitting light through a tissue specimen that has been stained in order to produce contrast. This process results in 2D images of the specimen that has a three-dimensional structure. In this paper, we…

Computer Vision and Pattern Recognition · Computer Science 2018-01-11 Maxime W. Lafarge , Josien P. W. Pluim , Koen A. J. Eppenhof , Pim Moeskops , Mitko Veta