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Spatial transcriptomics (ST) has revolutionised transcriptomics analysis by preserving tissue architecture, allowing researchers to study gene expression in its native spatial context. However, despite its potential, ST still faces…

Quantitative Methods · Quantitative Biology 2025-05-19 Anthony Baptista , Rosamond Nuamah , Ciro Chiappini , Anita Grigoriadis

Gene expression estimation from pathology images has the potential to reduce the RNA sequencing cost. Point-wise loss functions have been widely used to minimize the discrepancy between predicted and absolute gene expression values.…

Computer Vision and Pattern Recognition · Computer Science 2025-12-09 Kazuya Nishimura , Haruka Hirose , Ryoma Bise , Kaito Shiku , Yasuhiro Kojima

Quantitative characterization of cellular spatial organization is critical for understanding tumor progression and immune response. Recent advances in artificial intelligence (AI) enable large-scale segmentation and classification of nuclei…

The Resolution of feature maps is critical for medical image segmentation. Most of the existing Transformer-based networks for medical image segmentation are U-Net-like architecture that contains an encoder that utilizes a sequence of…

Computer Vision and Pattern Recognition · Computer Science 2022-07-26 Chen Wei , Shenghan Ren , Kaitai Guo , Haihong Hu , Jimin Liang

Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

Spatial transcriptomics (ST) technologies have revolutionized the study of gene expression patterns in tissues by providing multimodality data in transcriptomic, spatial, and morphological, offering opportunities for understanding tissue…

Computational Engineering, Finance, and Science · Computer Science 2024-01-17 Zelin Zang , Liangyu Li , Yongjie Xu , Chenrui Duan , Kai Wang , Yang You , Yi Sun , Stan Z. Li

In recent years, deep learning has presented a great advance in hyperspectral image (HSI) classification. Particularly, long short-term memory (LSTM), as a special deep learning structure, has shown great ability in modeling long-term…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Wen-Shuai Hu , Heng-Chao Li , Lei Pan , Wei Li , Ran Tao , Qian Du

Hyperspectral image (HSI) plays a vital role in various fields such as agriculture and environmental monitoring. However, due to the expensive acquisition cost, the number of hyperspectral images is limited, degenerating the performance of…

Computer Vision and Pattern Recognition · Computer Science 2024-11-04 Li Pang , Xiangyong Cao , Datao Tang , Shuang Xu , Xueru Bai , Feng Zhou , Deyu Meng

Highly accurate geometric precision and dense image features characterize True Digital Orthophoto Maps (TDOMs), which are in great demand for applications such as urban planning, infrastructure management, and environmental monitoring.…

Computer Vision and Pattern Recognition · Computer Science 2026-05-05 Qian Wang , Zhihao Zhan , Jialei He , Zhituo Tu , Jie Yuan

Hyperspectral image super-resolution has attained widespread prominence to enhance the spatial resolution of hyperspectral images. However, convolution-based methods have encountered challenges in harnessing the global spatial-spectral…

Image and Video Processing · Electrical Eng. & Systems 2023-11-30 Shi Chen , Lefei Zhang , Liangpei Zhang

In the analysis of spatially resolved transcriptomics data, detecting spatially variable genes (SVGs) is crucial. Numerous computational methods exist, but varying SVG definitions and methodologies lead to incomparable results. We review 33…

Quantitative Methods · Quantitative Biology 2024-10-04 Guanao Yan , Shuo Harper Hua , Jingyi Jessica Li

Spatial transcriptomics enables spatial gene expression profiling, motivating computational models that capture spatially conditioned regulatory relationships. We introduce SAGE-FM, a lightweight spatial transcriptomics foundation model…

Machine Learning · Computer Science 2026-01-23 Xianghao Zhan , Jingyu Xu , Yuanning Zheng , Zinaida Good , Olivier Gevaert

Histopathology plays a pivotal role in medical diagnostics. In contrast to preparing permanent sections for histopathology, a time-consuming process, preparing frozen sections is significantly faster and can be performed during surgery,…

Image and Video Processing · Electrical Eng. & Systems 2023-10-18 Elad Yoshai , Gil Goldinger , Miki Haifler , Natan T. Shaked

Cancer pathological analysis requires modeling tumor heterogeneity across multiple modalities, primarily through transcriptomics and whole slide imaging (WSI), along with their spatial relations. On one hand, bulk transcriptomics and WSI…

Machine Learning · Computer Science 2026-03-17 Jingkun Yu , Guangkai Shang , Changtao Li , Xun Gong , Tianrui Li , Yazhou He , Zhipeng Luo

Spatially resolved transcriptomics represents a significant advancement in single-cell analysis by offering both gene expression data and their corresponding physical locations. However, this high degree of spatial resolution entails a…

Genomics · Quantitative Biology 2024-03-19 Xiaoyu Li , Wenwen Min , Shunfang Wang , Changmiao Wang , Taosheng Xu

The advent of plant phenomics, coupled with the wealth of genotypic data generated by next-generation sequencing technologies, provides exciting new resources for investigations into and improvement of complex traits. However, these new…

Genomics · Quantitative Biology 2019-04-30 Gota Morota , Diego Jarquin , Malachy T. Campbell , Hiroyoshi Iwata

Deep learning has proven to successfully learn variations in tissue and cell morphology. Training of such models typically relies on expensive manual annotations. Here we conjecture that spatially resolved gene expression, e.i., the…

Quantitative Methods · Quantitative Biology 2023-12-11 Axel Andersson , Gabriele Partel , Leslie Solorzano , Carolina Wählby

Spatial transcriptomics reveals gene expression patterns within tissue context, enabling precision oncology applications such as treatment response prediction, but its high cost and technical complexity limit clinical adoption. Predicting…

Computer Vision and Pattern Recognition · Computer Science 2025-08-04 Won June Cho , Hongjun Yoon , Daeky Jeong , Hyeongyeol Lim , Yosep Chong

Understanding 3D fundamental processes is crucial for academic and industrial applications. Nowadays, X-ray time-resolved tomography, or tomoscopy, is a leading technique for in-situ and operando 4D (3D+time) characterization. Despite its…

Spatial transcriptomics (ST) has advanced significantly in the last few years. Such advancement comes with the urgent need for novel computational methods to handle the unique challenges of ST data analysis. Many artificial intelligence…

Machine Learning · Computer Science 2022-03-21 Yijun Li , Stefan Stanojevic , Lana X. Garmire