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Spatial transcriptomics (ST) technologies enable transcriptome-wide gene expression profiling while preserving spatial resolution, offering unprecedented opportunities to uncover complex spatial structures. Due to the ultra-high…

Applications · Statistics 2026-03-11 Changhu Wang , Qiyun Huang , Zihao Chen , Jin Liu , Ruibin Xi

Recent advancements in spatial transcriptomics technologies allow researchers to simultaneously measure RNA expression levels for hundreds to thousands of genes while preserving spatial information within tissues, providing critical…

Methodology · Statistics 2025-07-31 Catherine Higgins , Jingyi Jessica Li , Michelle Carey

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-01-25 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Spatial transcriptomics (ST) enables simultaneous mapping of tissue morphology and spatially resolved gene expression, offering unique opportunities to study tumor microenvironment heterogeneity. Here, we introduce a computational framework…

Quantitative Methods · Quantitative Biology 2026-04-27 Ling Liao , Changhuei Yang , Maxim Artyomov , Mark Watson , Adam Kepecs , Haowen Zhou , Alexey Sergushichev , Richard Cote

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-12-23 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

The rapid growth of digital pathology and advances in self-supervised deep learning have enabled the development of foundational models for various pathology tasks across diverse diseases. While multimodal approaches integrating diverse…

Computer Vision and Pattern Recognition · Computer Science 2025-10-15 Ekaterina Redekop , Mara Pleasure , Zichen Wang , Kimberly Flores , Anthony Sisk , William Speier , Corey W. Arnold

Spatially Resolved Transcriptomics (SRT) is a cutting-edge technique that captures the spatial context of cells within tissues, enabling the study of complex biological networks. Recent graph-based methods leverage both gene expression and…

Machine Learning · Computer Science 2025-06-24 Yunhak Oh , Junseok Lee , Yeongmin Kim , Sangwoo Seo , Namkyeong Lee , Chanyoung Park

Spatial Transcriptomics (ST) offers spatially resolved gene expression but remains costly. Predicting expression directly from widely available Hematoxylin and Eosin (H&E) stained images presents a cost-effective alternative. However, most…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Jiarui Ouyang , Yihui Wang , Yihang Gao , Yingxue Xu , Shu Yang , Hao Chen

Spatial transcriptomics studies are becoming increasingly large and commonplace, necessitating simultaneous analysis of a large number of spatially resolved variables. Correspondingly, a diverse range of methodologies have been proposed to…

Quantitative Methods · Quantitative Biology 2025-09-09 James Boyle , Gregory Hamm , Eleanor Williams , Robin JG Hartman , Magnus Soderburg , Ian Henry , Michael Casey

Spatial transcriptomics (ST) profiles gene expression across a tissue section while preserving the spatial coordinates. Because current ST technologies typically profile two-dimensional tissue slices, integrating and aligning slices from…

Quantitative Methods · Quantitative Biology 2026-03-20 Yaqi Wu , Jingfeng Wang , Xin Maizie Zhou , Yanxiang Zhao , Zixuan Cang

Single-cell transcriptomics and proteomics have become a great source for data-driven insights into biology, enabling the use of advanced deep learning methods to understand cellular heterogeneity and gene expression at the single-cell…

Genomics · Quantitative Biology 2025-12-15 Hiren Madhu , João Felipe Rocha , Tinglin Huang , Siddharth Viswanath , Smita Krishnaswamy , Rex Ying

The technology to generate Spatially Resolved Transcriptomics (SRT) data is rapidly being improved and applied to investigate a variety of biological tissues. The ability to interrogate how spatially localised gene expression can lend new…

Quantitative Methods · Quantitative Biology 2021-08-04 Natalie Charitakis , Mirana Ramialison , Hieu T. Nim

Spatially resolved transcriptomics is a fast-developing set of technologies that enables the measurement of localized gene expression across spatial locations in a sample. Detecting spatially varying genes is critical for analyzing such…

Applications · Statistics 2026-04-22 Pritam Dey , Rajarshi Guhaniyogi , Yang Ni , Bani K. Mallick

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min

Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Spatial transcriptomics (ST) enables mapping gene expression with spatial context but is severely affected by high sparsity and technical noise, which conceals true biological signals and hinders downstream analyses. To address these…

Machine Learning · Computer Science 2026-03-10 Sayeem Bin Zaman , Fahim Hafiz , Riasat Azim

Predicting spatial gene expression from H&E histology offers a scalable and clinically accessible alternative to sequencing, but realizing clinical impact requires models that generalize across cancer types and capture biologically coherent…

Machine Learning · Computer Science 2026-02-10 Susu Hu , Qinghe Zeng , Nithya Bhasker , Jakob Nikolas Kather , Stefanie Speidel

Spatial transcriptomics (ST) has emerged as an advanced technology that provides spatial context to gene expression. Recently, deep learning-based methods have shown the capability to predict gene expression from WSI data using ST data.…

Machine Learning · Computer Science 2024-12-10 Mingcheng Qu , Yuncong Wu , Donglin Di , Anyang Su , Tonghua Su , Yang Song , Lei Fan

Identifying disease-indicative genes is critical for deciphering disease mechanisms and has attracted significant interest in biomedical research. Spatial transcriptomics offers unprecedented insights for the detection of disease-specific…

Methodology · Statistics 2024-09-05 Qicheng Zhao , Qihuang Zhang