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Related papers: Complementary Speckle STED Microscopy

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We introduce the concept of saturated absorption competition (SAC) microscopy as a means of providing sub-diffraction spatial resolution in fluorescence imaging. Unlike the post-competition process between stimulated and spontaneous…

Intensity minima and maxima of speckle patterns obtained behind a diffuser are experimentally interchanged by applying a spiral phase delay of charge $\pm 1$ to the impinging coherent beam. This transform arises from the intuitive…

Optics · Physics 2017-02-01 Jérôme Gateau , Hervé Rigneault , Marc Guillon

Speckle patterns have been widely used in imaging techniques such as ghost imaging, dynamic speckle illumination microscopy, structured illumination microscopy, and photoacoustic fluctuation imaging. Recent advances in the ability to…

Optics · Physics 2021-05-28 Nicholas Bender , Mengyuan Sun , Hasan Yilmaz , Joerg Bewersdorf , Hui Cao

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Spectral imaging enables the analysis of optical material properties that are invisible to the human eye. Different spectral capturing setups, e.g., based on filter-wheel, push-broom, line-scanning, or mosaic cameras, have been introduced…

Image and Video Processing · Electrical Eng. & Systems 2024-10-01 Eric L. Wisotzky , Jost Triller , Anna Hilsmann , Peter Eisert

I propose a spatial-mode demultiplexing (SPADE) measurement scheme for the far-field imaging of spatially incoherent optical sources. For any object too small to be resolved by direct imaging under the diffraction limit, I show that SPADE…

Optics · Physics 2017-03-01 Mankei Tsang

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

This is the first article in a series of three dealing with the exploitation of speckle for imaging purposes. Speckle is the complex interference wave-field produced by a random distribution of un-resolved scatterers. In this paper, we show…

Applied Physics · Physics 2026-02-06 Elsa Giraudat , Flavien Bureau , William Lambert , Mathias Fink , Alexandre Aubry

Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot…

Biological Physics · Physics 2015-01-05 Rumelo Amor , Sumeet Mahajan , William Bradshaw Amos , Gail McConnell

Super-resolution effects in optical and fluorescence biological systems are analyzed and their relations with uncertainty relations are discussed. Super-resolutions obtained in the optical systems, including especially NSOM, SIM and…

Optics · Physics 2015-06-12 Yacob Ben-Aryeh

Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…

Instrumentation and Methods for Astrophysics · Physics 2014-09-17 Arun Surya , Swapan K. Saha , Antoine Labeyrie

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

The development of four-dimensional (4D) scanning transmission electron microscopy (STEM) using fast detectors has opened-up new avenues for addressing some of long-standing challenges in electron imaging. One of these challenges is how to…

Materials Science · Physics 2021-01-19 Yu-Tsun Shao , Renliang Yuan , Haw-Wen Hsiao , Qun Yang , Yang Hu , Jian-Min Zuo

Magnetic particle imaging (MPI) is an in vivo method to detect magnetic nanoparticles for cell tracking, vascular imaging, and molecular target imaging without ionizing radiation. Current magnetic particle imaging is accomplished by forming…

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

This work is concerned with optical imaging in strongly diffusive environments. We consider a typical setting in optical coherence tomography where a sample is probed by a collection of wavefields produced by a laser and propagating through…

Optics · Physics 2024-12-10 Randy Bartels , Olivier Pinaud , Maxine Varughese

4D-STEM, in which the 2D diffraction plane is captured for each 2D scan position in the scanning transmission electron microscope (STEM) using a pixelated detector, is complementing and increasingly replacing existing imaging approaches.…

Instrumentation and Detectors · Physics 2022-07-27 Colum M. O'Leary , Benedikt Haas , Christoph T. Koch , Peter D. Nellist , Lewys Jones

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…