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Understanding chromatin organization and dynamics is important since they crucially affect DNA functions. In this study, we investigate chromatin dynamics by statistically analyzing single-nucleosome movement in living human cells. Bi-modal…

Soft Condensed Matter · Physics 2022-10-12 S. S. Ashwin , Tadasu Nozaki , Kazuhiro Maeshima , Masaki Sasai

Chromatin is a complex of DNA, RNA and proteins whose primary function is to package genomic DNA into the tight confines of a cell nucleus. A fundamental repeating unit of chromatin is the nucleosome, an octamer of histone proteins around…

Genomics · Quantitative Biology 2012-06-21 Denis Tolkunov , Alexandre V. Morozov

Intact-organism imaging of Drosophila larvae reveals and quantifies chromatin-aqueous phase separation. The chromatin can be organized near the lamina layer of the nuclear envelope, conventionally fill the nucleus, be organized centrally,…

Biological Physics · Physics 2020-10-21 Gaurav Bajpai , Daria Amiad-Pavlov , Dana Lorber , Talila Volk , Samuel Safran

Chromatin moves dynamically inside the cell nucleus, and its motion is often correlated with gene functions such as DNA recombination and transcription. A recent study has shown that during early embryogenesis of the nematode,…

Biological Physics · Physics 2021-03-09 Aiya K. Yesbolatova , Ritsuko Arai , Takahiro Sakaue , Akatsuki Kimura

Protein-mediated interactions are ubiquitous in the cellular environment, and particularly in the nucleus, where they are responsible for the structuring of chromatin. We show through molecular--dynamics simulations of a polymer surrounded…

Biomolecules · Quantitative Biology 2024-03-21 Francesco Borando , Guido Tiana

Lamina-associated domains (LADs) cover a large part of the human genome and are thought to play a major role in shaping the nuclear architectural landscape. Here, we perform polymer simulations, microscopy and mass spectrometry to dissect…

During interphase, a typical cell nucleus features spatial compartmentalization of transcriptionally active euchromatin and repressed heterochromatin domains. In conventional nuclear organization, euchromatin predominantly occupies the…

Soft Condensed Matter · Physics 2025-07-25 Ligesh Theeyancheri , Edward J. Banigan , J. M. Schwarz

The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus and the coupling with the cytoskeleton. Nuclear alterations are often associated…

The architecture of the eukaryotic genome is characterized by a high degree of spatial organization. Chromosomes occupy preferred territories correlated to their state of activity and, yet, displace their genes to interact with remote sites…

Genomics · Quantitative Biology 2015-05-14 Mario Nicodemi , Antonella Prisco

The cell nucleus houses the chromosomes, which are linked to a soft shell of lamin filaments. Experiments indicate that correlated chromosome dynamics and nuclear shape fluctuations arise from motor activity. To identify the physical…

Subcellular Processes · Quantitative Biology 2021-04-21 Kuang Liu , Alison E. Patteson , Edward J. Banigan , J. M. Schwarz

A living cell is an active environment where the organization and dynamics of chromatin are affected by different forms of activity. Optical experiments report that loci show subdiffusive dynamics and the chromatin fiber is seen to be…

Soft Condensed Matter · Physics 2023-03-15 Subhasish Chaki , Ligesh Theeyancheri , Rajarshi Chakrabarti

Fluid-structure interactions between active and passive components are important for many biological systems to function. A particular example is chromatin in the cell nucleus, where ATP-powered processes drive coherent motions of the…

Soft Condensed Matter · Physics 2024-02-15 Scott Weady , David B. Stein , Alexandra Zidovska , Michael J. Shelley

We discover a new type of nonequilibrium phase transition in a model of chromatin dynamics, which accounts for the coherent motions that have been observed in experiment. The coherent motion is due to the long-range cooperation of molecular…

Soft Condensed Matter · Physics 2023-07-27 Iraj Eshghi , Alexanda Zidovska , Alexander Y. Grosberg

We present a theoretical model of facilitated diffusion of proteins in the cell nucleus. This model, which takes into account the successive binding/unbinding events of proteins to DNA, relies on a fractal description of the chromatin which…

Statistical Mechanics · Physics 2015-05-19 O. Benichou , C. Chevalier , B. Meyer , R. Voituriez

Chromatin is a highly compact and dynamic nuclear structure that consists of DNA and associated proteins. The main organizational unit is the nucleosome, which consists of a histone octamer with DNA wrapped around it. Histone proteins are…

Genomics · Quantitative Biology 2013-09-30 Veit Schwämmle , Ole Nørregaard Jensen

Apart from being the gateway for all access to the eukaryotic genome, chromatin has in recent years been identified as carrying an epigenetic code regulating transcriptional activity. The detailed knowledge of this code contrasts the…

Other Quantitative Biology · Quantitative Biology 2007-12-07 Martin Depken , Helmut Schiessel

The development of multicellular organisms relies on the precise coordination of molecular events across multiple spatial and temporal scales. Understanding how information flows from molecular interactions to cellular processes and tissue…

Biological Physics · Physics 2025-01-29 Andrés H. Cardona , Márcia Mesquita Peixoto , Tohn Borjigin , Thomas Gregor

Spatiotemporal coordination of chromatin and subnuclear compartments is crucial for cells. Numerous enzymes act inside nucleus\textemdash some of those transiently link and pass two chromatin segments. Here we study how such an active…

Biological Physics · Physics 2024-01-18 Rakesh Das , Takahiro Sakaue , G. V. Shivashankar , Jacques Prost , Tetsuya Hiraiwa

The connection between chromatin nuclear organization and gene activity is vividly illustrated by the observation that transcriptional coregulation of certain genes appears to be directly influenced by their spatial proximity. This fact…

Genomics · Quantitative Biology 2015-06-15 Marco Di Stefano , Angelo Rosa , Vincenzo Belcastro , Diego di Bernardo , Cristian Micheletti

Dynamics of nucleosomes, the building blocks of the chromatin, has crucial effects on expression, replication and repair of genomes in eukaryotes. Beside constant movements of nucleosomes by thermal fluctuations, ATP-dependent chromatin…

Biomolecules · Quantitative Biology 2020-01-28 Fatemeh Khodabandeh , Hashem Fatemi , Farshid Mohammad-Rafiee1
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