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We present a non-iterative method to deconvolve the spatial response function or the point spread function (PSF) from images taken with the Suzaku X-ray Imaging Spectrometer (XIS). The method is optimized for analyses of extended sources…

Astrophysics · Physics 2015-05-13 Mutsumi Sugizaki , Tuneyoshi Kamae , Yoshitomo Maeda

Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…

Biological Physics · Physics 2018-06-12 Mathias Lechelon , Marco Pettini

Quantifying formidable multiple coupling effects involved in Surface-enhanced Raman scattering (SERS) is a prerequisite for accurate design of SERS probes with superior detection limit and uniformity which are the targets for trace…

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

Quantitative Methods · Quantitative Biology 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Sachin Shah , Sakshum Kulshrestha , Christopher A. Metzler

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

Single molecule X-ray scattering experiments using free electron lasers hold the potential to resolve both single structures and structural ensembles of biomolecules. However, molecular electron density determination has so far not been…

Computational Physics · Physics 2026-04-17 Steffen Schultze , Helmut Grubmüller

Scattering-based single-particle tracking (S-SPT) has opened new avenues for highly sensitive label-free detection and characterization of nanoscopic objects, making it particularly attractive for various analytical applications. However, a…

Modeling the Point Spread Function (PSF) of wide-field surveys is vital for many astrophysical applications and cosmological probes including weak gravitational lensing. The PSF smears the image of any recorded object and therefore needs to…

Instrumentation and Methods for Astrophysics · Physics 2018-07-26 Jörg Herbel , Tomasz Kacprzak , Adam Amara , Alexandre Refregier , Aurelien Lucchi

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

Since model quantization helps to reduce the model size and computation latency, it has been successfully applied in many applications of mobile phones, embedded devices and smart chips. The mixed-precision quantization model can match…

Computer Vision and Pattern Recognition · Computer Science 2021-03-05 Qigong Sun , Licheng Jiao , Yan Ren , Xiufang Li , Fanhua Shang , Fang Liu

The first step toward doing high-precision astrometry is the measurement of individual stars in individual images, a step that is fraught with dangers when the images are undersampled. The key to avoiding systematic positional error in…

Astrophysics · Physics 2009-10-05 Jay Anderson , Ivan R. King

Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

Point spread function (PSF) reconstruction (PSF-R) is a well established technique to determine reliably and accurately the PSF from Adaptive Optics (AO) control loop data. We have successfully applied this technique to improve the…

Instrumentation and Methods for Astrophysics · Physics 2020-04-15 Olivier Beltramo-Martin , Antonino Marasco , Thierry Fusco , Davide Massari , Julien Milli , Giuliana Fiorentino , B. Neichel

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

The localization problem in a wireless sensor network is to determine the coordination of sensor nodes using the known positions of some nodes (called anchors) and corresponding noisy distance measurements. There is a variety of different…

Optimization and Control · Mathematics 2014-09-19 Pouya Mollaebrahim Ghari , Reza Shahbazian , Seyed Ali Ghorashi

A new technical method is developed for soft x-ray spectroscopy of near-edge x-ray absorption fine structure (NEXAFS). The measurement is performed with continuously rotating linearly polarized light over 2$\pi$, generated by a segmented…

Instrumentation and Detectors · Physics 2021-11-10 Yoshiki Kudo , Yasuyuki Hirata , Masafumi Horio , Masahito Niibe , Iwao Matsuda

There are still two problems in SDD causing some inaccurate results: (1) In the process of feature extraction, with the layer-by-layer acquisition of semantic information, local information is gradually lost, resulting into less…

Computer Vision and Pattern Recognition · Computer Science 2022-10-11 Aisha Chandio , Gong Gui , Teerath Kumar , Irfan Ullah , Ramin Ranjbarzadeh , Arunabha M Roy , Akhtar Hussain , Yao Shen

In this paper, we propose a molecular communication system to localize an abnormality in a diffusion based medium. We consider a general setup to perform joint sensing, communication and localization. This setup consists of three types of…

Signal Processing · Electrical Eng. & Systems 2022-04-01 Ladan Khaloopour , Mahtab Mirmohseni , Masoumeh Nasiri-Kenari
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