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The emergence of virtual staining technology provides a rapid and efficient alternative for researchers in tissue pathology. It enables the utilization of unlabeled microscopic samples to generate virtual replicas of chemically stained…

Image and Video Processing · Electrical Eng. & Systems 2025-01-08 Junjia Wang , Bo Xiong , You Zhou , Xun Cao , Zhan Ma

Deep learning has been extensively used in various fields, such as phase imaging, 3D imaging reconstruction, phase unwrapping, and laser speckle reduction, particularly for complex problems that lack analytic models. Its data-driven nature…

Machine Learning · Computer Science 2024-10-16 Xuyu Zhang , Haofan Huang , Dawei Zhang , Songlin Zhuang , Shensheng Han , Puxiang Lai , Honglin Liu

Whole Slide Images (WSIs) in digital pathology are used to diagnose cancer subtypes. The difference in procedures to acquire WSIs at various trial sites gives rise to variability in the histopathology images, thus making consistent…

Image and Video Processing · Electrical Eng. & Systems 2022-04-07 Milad Sikaroudi , Shahryar Rahnamayan , H. R. Tizhoosh

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

The presence and density of specific types of immune cells are important to understand a patient's immune response to cancer. However, immunofluorescence staining required to identify T cell subtypes is expensive, time-consuming, and rarely…

Computer Vision and Pattern Recognition · Computer Science 2022-10-18 Georg Wölflein , In Hwa Um , David J Harrison , Ognjen Arandjelović

Digitized Histological diagnosis is in increasing demand. However, color variations due to various factors are imposing obstacles to the diagnosis process. The problem of stain color variations is a well-defined problem with many proposed…

Computer Vision and Pattern Recognition · Computer Science 2018-04-06 M Tarek Shaban , Christoph Baur , Nassir Navab , Shadi Albarqouni

In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

Computer Vision and Pattern Recognition · Computer Science 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

Computer Vision and Pattern Recognition · Computer Science 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

Hematoxylin and Eosin stained histopathology image analysis is essential for the diagnosis and study of complicated diseases such as cancer. Existing state-of-the-art approaches demand extensive amount of supervised training data from…

Computer Vision and Pattern Recognition · Computer Science 2017-12-15 Le Hou , Ayush Agarwal , Dimitris Samaras , Tahsin M. Kurc , Rajarsi R. Gupta , Joel H. Saltz

To enable personalized cancer treatment, machine learning models have been developed to predict drug response as a function of tumor and drug features. However, most algorithm development efforts have relied on cross validation within a…

Real-world deployment of computer vision systems, including in the discovery processes of biomedical research, requires causal representations that are invariant to contextual nuisances and generalize to new data. Leveraging the internal…

Computer Vision and Pattern Recognition · Computer Science 2023-06-22 Wolfgang M. Pernice , Michael Doron , Alex Quach , Aditya Pratapa , Sultan Kenjeyev , Nicholas De Veaux , Michio Hirano , Juan C. Caicedo

While human observers are able to cope with variations in color and appearance of histological stains, digital pathology algorithms commonly require a well-normalized setting to achieve peak performance, especially when a limited amount of…

Computer Vision and Pattern Recognition · Computer Science 2017-08-15 Daniel Bug , Steffen Schneider , Anne Grote , Eva Oswald , Friedrich Feuerhake , Julia Schüler , Dorit Merhof

Recent breakthroughs in self-supervised learning have enabled the use of large unlabeled datasets to train visual foundation models that can generalize to a variety of downstream tasks. While this training paradigm is well suited for the…

Stain variation is a phenomenon observed when distinct pathology laboratories stain tissue slides that exhibit similar but not identical color appearance. Due to this color shift between laboratories, convolutional neural networks (CNNs)…

Computer Vision and Pattern Recognition · Computer Science 2020-04-16 David Tellez , Geert Litjens , Peter Bandi , Wouter Bulten , John-Melle Bokhorst , Francesco Ciompi , Jeroen van der Laak

Adopting Convolutional Neural Networks (CNNs) in the daily routine of primary diagnosis requires not only near-perfect precision, but also a sufficient degree of generalization to data acquisition shifts and transparency. Existing CNN…

Computer Vision and Pattern Recognition · Computer Science 2023-06-22 Mara Graziani , Sebastian Otalora , Stephane Marchand-Maillet , Henning Muller , Vincent Andrearczyk

Increased levels of tumor infiltrating lymphocytes (TILs) in cancer tissue indicate favourable outcomes in many types of cancer. Manual quantification of immune cells is inaccurate and time consuming for pathologists. Our aim is to leverage…

Histological Tertiary Lymphoid Structures (TLSs) are increasingly recognized for their correlation with the efficacy of immunotherapy in various solid tumors. Traditionally, the identification and characterization of TLSs rely on…

Image and Video Processing · Electrical Eng. & Systems 2024-08-27 Qiuli Wang , Yongxu Liu , Li Ma , Xianqi Wang , Wei Chen , Xiaohong Yao

Many practical medical imaging scenarios include categories that are under-represented but still crucial. The relevance of image recognition models to real-world applications lies in their ability to generalize to these rare classes as well…

Computer Vision and Pattern Recognition · Computer Science 2025-05-22 Daniya Najiha A. Kareem , Jean Lahoud , Mustansar Fiaz , Amandeep Kumar , Hisham Cholakkal

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

While deep learning has seen many recent applications to drug discovery, most have focused on predicting activity or toxicity directly from chemical structure. Phenotypic changes exhibited in cellular images are also indications of the…

Computer Vision and Pattern Recognition · Computer Science 2019-03-18 Philip T. Jackson , Yinhai Wang , Sinead Knight , Hongming Chen , Thierry Dorval , Martin Brown , Claus Bendtsen , Boguslaw Obara