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When our immune system encounters foreign antigens (i.e., from pathogens), the B cells that produce our antibodies undergo a cyclic process of proliferation, mutation, and selection, improving their ability to bind to the specific antigen.…

Label-free approaches are attractive in cytological imaging due to their flexibility and cost efficiency. They are supported by machine learning methods, which, despite the lack of labeling and the associated lower contrast, can classify…

Computer Vision and Pattern Recognition · Computer Science 2023-11-27 Stefan Röhrl , Johannes Groll , Manuel Lengl , Simon Schumann , Christian Klenk , Dominik Heim , Martin Knopp , Oliver Hayden , Klaus Diepold

As interpretability has been pointed out as the obstacle to the adoption of Deep Neural Networks (DNNs), there is an increasing interest in solving a transparency issue to guarantee the impressive performance. In this paper, we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-07-20 Woo-Jeoung Nam , Seong-Whan Lee

An analysis of the network defined by the potential energy minima of multi-atomic systems and their connectivity via reaction pathways that go through transition states allows to understand important characteristics like thermodynamic,…

Materials Science · Physics 2016-08-03 Bastian Schaefer , Stefan Goedecker

The accurate classification of lymphoma subtypes using hematoxylin and eosin (H&E)-stained tissue is complicated by the wide range of morphological features these cancers can exhibit. We present LymphoML - an interpretable machine learning…

The major histocompatibility complex (MHC) molecules, which bind peptides for presentation on the cell surface, play an important role in cell-mediated immunity. In light of developing databases and technologies over the years, significant…

Biomolecules · Quantitative Biology 2023-01-26 Ayşenaz Ezgi Ergin , Deniz Turgay Altılar

Reliable predictions from systems biology models require knowing whether parameters can be estimated from available data, and with what certainty. Identifiability analysis reveals whether parameters are learnable in principle (structural…

Formulating accurate and robust classification strategies is a key challenge of developing diagnostic and antibody tests. Methods that do not explicitly account for disease prevalence and uncertainty therein can lead to significant…

Methodology · Statistics 2022-02-01 Paul N. Patrone , Anthony J. Kearsley

Antibodies are Y-shaped proteins that protect the host by binding to specific antigens, and their binding is mainly determined by the Complementary Determining Regions (CDRs) in the antibody. Despite the great progress made in CDR design,…

Quantitative Methods · Quantitative Biology 2025-01-03 Lirong Wu , Haitao Lin , Yufei Huang , Zhangyang Gao , Cheng Tan , Yunfan Liu , Tailin Wu , Stan Z. Li

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Doping control is an essential component of anti-doping organizations for protecting clean sports competitions. Since 2009, this mission has been complemented worldwide by the Athlete Biological Passport (ABP), used to monitor athletes'…

Methodology · Statistics 2024-04-22 Nina Deliu , Brunero Liseo

The limits of molecular dynamics (MD) simulations of macromolecules are steadily pushed forward by the relentless developments of computer architectures and algorithms. This explosion in the number and extent (in size and time) of MD…

Nowadays, the amount of heterogeneous biomedical data is increasing more and more thanks to novel sensing techniques and high-throughput technologies. In reference to biomedical image analysis, the advances in image acquisition modalities…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Leonardo Rundo

An accurate binding affinity prediction between T-cell receptors and epitopes contributes decisively to develop successful immunotherapy strategies. Some state-of-the-art computational methods implement deep learning techniques by…

Machine Learning · Computer Science 2024-01-18 Etienne Goffinet , Raghvendra Mall , Ankita Singh , Rahul Kaushik , Filippo Castiglione

We provide a computational method for quickly determining the correct distribution of optically active nanoparticles for a desired response. This is achieved by simulating the optical response of single nanoparticles and performing a…

Optics · Physics 2019-01-03 Phillip Manley , Min Song , Sven Burger , Martina Schmid

In this paper we consider a statistical estimation problem known as atomic deconvolution. Introduced in reliability, this model has a direct application when considering biological data produced by flow cytometers. In these experiments,…

Statistics Theory · Mathematics 2017-10-12 Manon Costa , Sébastien Gadat , Pauline Gonnord , Laurent Risser

We introduce a methodology for performing parameter inference in high-dimensional, non-linear diffusion processes. We illustrate its applicability for obtaining insights into the evolution of and relationships between species, including…

Machine Learning · Statistics 2024-11-15 Nicklas Boserup , Gefan Yang , Michael Lind Severinsen , Christy Anna Hipsley , Stefan Sommer

Motivation: In silico methods for the prediction of antigenic peptides binding to MHC class I molecules play an increasingly important role in the identification of T-cell epitopes. Statistical and machine learning methods, in particular,…

Quantitative Methods · Quantitative Biology 2007-05-23 Laurent Jacob , Jean-Philippe Vert

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

Monoclonal antibodies are among the most promising therapeutic agents in modern medicine, yet their formulation into high-concentration solutions for subcutaneous self-administration poses a major challenge. A key obstacle is the marked…

Soft Condensed Matter · Physics 2025-01-14 Fabrizio Camerin , Marco Polimeni , Anna Stradner , Emanuela Zaccarelli , Peter Schurtenberger