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Deblurring is a fundamental inverse problem in bioimaging. It requires modelling the point spread function (PSF), which captures the optical distortions entailed by the image formation process. The PSF limits the spatial resolution…

Image and Video Processing · Electrical Eng. & Systems 2019-03-04 Denis K. Samuylov , Prateek Purwar , Gábor Székely , Grégory Paul

We present a new algorithm for estimating the Point Spread Function (PSF) in wide-field astronomical images with extreme source crowding. Robust and accurate PSF estimation in crowded astronomical images dramatically improves the fidelity…

Image and Video Processing · Electrical Eng. & Systems 2021-02-09 Brendt Wohlberg , Przemek Wozniak

We present a novel technique to assess the focal volume of petawatt-class lasers at full power. Our approach exploits quantitative measurement of the angular distribution of electrons born in the focus via ionization of rarefied gas, which…

Single Particle Tracking (SPT) is a powerful class of tools for analyzing the dynamics of individual biological macromolecules moving inside living cells. The acquired data is typically in the form of a sequence of camera images that are…

Biomolecules · Quantitative Biology 2020-01-23 Ye Lin , Sean B. Andersson

A new method is presented for determining the Point Spread Function (PSF) of images that lack bright and isolated stars. It is based on the same principles as the MCS (Magain, Courbin, Sohy, 1998) image deconvolution algorithm. It uses the…

Astrophysics · Physics 2009-11-11 P. Magain , F. Courbin , M. Gillon , S. Sohy , G. Letawe , V. Chantry , Y. Letawe

In order to enhance accuracy of astrophysical estimates obtained on Adaptive-optics (AO) images, such as photometry and astrometry, we investigate a new concept to constrain the Point Spread Function (PSF) model called PSF Reconstruction…

Instrumentation and Methods for Astrophysics · Physics 2019-06-26 O. Beltramo-Martin , C. M. Correia , S. Ragland , L. Jolissaint , B. Neichel , T. Fusco , P. L. Wizinowich

The evolution of the scanning modules for scanning transmission electron microscopes (STEM) has realized the possibility to generate arbitrary scan pathways, an approach currently explored to improve acquisition speed and to reduce electron…

Scanning probe microscopy (SPM) is ubiquitous in nanoscale science allowing the observation of features in real space down to the angstrom resolution. The scanning nature of SPM, wherein a sharp tip rasters the surface during which a…

Instrumentation and Detectors · Physics 2024-11-20 Maxime Le Ster , Sławomir Pawłowski , Iaroslav Lutsyk , Paweł Janusz Kowalczyk

Despite the widespread use of Scanning Transmission Electron Microscopy (STEM) for observing the structure of materials at the atomic scale, a detailed understanding of some relevant electron beam damage mechanisms is limited. Recent…

In this work, we present a novel technique to directly measure the phase shift of the optical signal scattered by single plasmonic nanoparticles in a diffraction-limited laser focus. We accomplish this by equipping an inverted confocal…

The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…

Optics · Physics 2022-09-07 Lukas Wesemann , Jon Rickett , Timothy J. Davis , Ann Roberts

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

The influence of low-spatial frequency errors of an optical component of an imaging system on the point spread function can be quantified using Zernike polynomials. High-spatial frequency errors cause strong scattering due to which the…

Optics · Physics 2026-04-03 Luuk Zonneveld , Paul Urbach , Aurèle Adam

The performance of electron energy-loss spectrometers can often be limited by their electron-optical aberrations. Due to recent developments in high energy-resolution and momentum-resolved electron energy loss spectroscopy (EELS), there is…

Materials Science · Physics 2023-02-28 Yueming Guo , Andrew R. Lupini

Low voltage transmission electron microscopy (<=80 kV) has many applications in imaging beam-sensitive samples, such as metallic nanoparticles, which may become damaged at higher voltages. To improve resolution, spherical aberration can be…

Instrumentation and Detectors · Physics 2022-07-27 Frances Quigley , Patrick McBean , Peter O'Donovan , Jonathan J. P. Peters , Lewys Jones

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

A non iterative direct blind deconvolution procedure, previously used successfully to sharpen Hubble Space Telescope imagery, is now found useful in sharpening nanoscale scanning electron microscope (SEM) and helium ion microscope (HIM)…

Instrumentation and Detectors · Physics 2025-02-28 Alfred S. Carasso , Andras E. Vladar

With the arrival of the next generation of ultra-deep optical imaging surveys reaching $\mu_V$$\sim$30 mag/arcsec$^2$ (3$\sigma$; 10"$\times$10"), the removal of scattered light due to the point spread function (PSF) effect remains a…

We perform spectroscopy on the hyperfine splitting of $^{85}$Rb atoms trapped in far-off-resonance optical traps. The existence of a spatially dependent shift in the energy levels is shown to induce an inherent dephasing effect, which…

Atomic Physics · Physics 2016-08-16 A. Kaplan , M. F. Andersen , T. Grünzweig , N. Davidson

Pump-probe spectroscopy is central for exploring ultrafast dynamics of fundamental excitations, collective modes and energy transfer processes. Typically carried out using conventional diffraction-limited optics, pump-probe experiments…