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Cell imaging assays utilizing fluorescence stains are essential for observing sub-cellular organelles and their responses to perturbations. Immunofluorescent staining process is routinely in labs, however the recent innovations in…

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

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Generative models have been applied in the medical imaging domain for various image recognition and synthesis tasks. However, a more controllable and interpretable image synthesis model is still lacking yet necessary for important…

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Performance of deep learning algorithms decreases drastically if the data distributions of the training and testing sets are different. Due to variations in staining protocols, reagent brands, and habits of technicians, color variation in…

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Manual counting of mitotic tumor cells in tissue sections constitutes one of the strongest prognostic markers for breast cancer. This procedure, however, is time-consuming and error-prone. We developed a method to automatically detect…

Developing self-supervised learning (SSL) models that can learn universal and transferable representations of H&E gigapixel whole-slide images (WSIs) is becoming increasingly valuable in computational pathology. These models hold the…

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Deep learning models can generate virtual immunohistochemistry (IHC) stains from hematoxylin and eosin (H&E) images, offering a scalable and low-cost alternative to laboratory IHC. However, reliable evaluation of image quality remains a…

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The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

Variability in staining protocols, such as different slide preparation techniques, chemicals, and scanner configurations, can result in a diverse set of whole slide images (WSIs). This distribution shift can negatively impact the…

Image and Video Processing · Electrical Eng. & Systems 2023-04-25 Kudaibergen Abutalip , Numan Saeed , Mustaqeem Khan , Abdulmotaleb El Saddik

Hematoxylin and eosin (H&E) staining is the clinical standard for assessing tissue morphology, but it lacks molecular-level diagnostic information. In contrast, immunohistochemistry (IHC) provides crucial insights into biomarker expression,…

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Artificial Intelligence (AI) has great potential to improve health outcomes by training systems on vast digitized clinical datasets. Computational Pathology, with its massive amounts of microscopy image data and impact on diagnostics and…

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The process of digitising histology slides involves multiple factors that can affect a whole slide image's (WSI) final appearance, including the staining protocol, scanner, and tissue type. This variability constitutes a domain shift and…

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Molecular testing of tumor samples for targetable biomarkers is restricted by a lack of standardization, turnaround-time, cost, and tissue availability across cancer types. Additionally, targetable alterations of low prevalence may not be…

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It is a critical task to evalaute HER2 expression level accurately for breast cancer evaluation and targeted treatment therapy selection. However, the standard multi-step Immunohistochemistry (IHC) staining is resource-intensive, expensive,…

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Artificial Intelligence in healthcare is a new and exciting frontier and the possibilities are endless. With deep learning approaches beating human performances in many areas, the logical next step is to attempt their application in the…

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Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

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Renal pathology, as the gold standard of kidney disease diagnosis, requires doctors to analyze a series of tissue slices stained by H&E staining and special staining like Masson, PASM, and PAS, respectively. These special staining methods…

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Quality assurance for large-scale cyber-physical systems relies on sophisticated test activities using complex test environments investigated with the help of numerous types of simulators. As these systems grow, extensive resources are…

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In digital pathology, different staining procedures and scanners cause substantial color variations in whole-slide images (WSIs), especially across different laboratories. These color shifts result in a poor generalization of deep…

Image and Video Processing · Electrical Eng. & Systems 2021-07-27 Sophia J. Wagner , Nadieh Khalili , Raghav Sharma , Melanie Boxberg , Carsten Marr , Walter de Back , Tingying Peng

Deep learning models that are trained on histopathological images obtained from a single lab and/or scanner give poor inference performance on images obtained from another scanner/lab with a different staining protocol. In recent years,…

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