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Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…

Unsupervised anomaly detection has gained significant attention in the field of medical imaging due to its capability of relieving the costly pixel-level annotation. To achieve this, modern approaches usually utilize generative models to…

Image and Video Processing · Electrical Eng. & Systems 2024-01-22 Rui Xu , Yunke Wang , Bo Du

We present an approach for multimodal pathology image search, using dynamic time warping (DTW) on Variational Autoencoder (VAE) latent space that is fed into a ranked choice voting scheme to retrieve multiplexed immunofluorescent imaging…

Tissue phenotyping is a fundamental task in learning objective characterizations of histopathologic biomarkers within the tumor-immune microenvironment in cancer pathology. However, whole-slide imaging (WSI) is a complex computer vision in…

Computer Vision and Pattern Recognition · Computer Science 2022-03-02 Richard J. Chen , Rahul G. Krishnan

Medical image segmentation is a relevant problem, with deep learning being an exponent. However, the necessity of a high volume of fully annotated images for training massive models can be a problem, especially for applications whose images…

Computer Vision and Pattern Recognition · Computer Science 2024-06-06 Matheus A. Cerqueira , Flávia Sprenger , Bernardo C. A. Teixeira , Alexandre X. Falcão

Multiplexed immuno-fluorescence tissue imaging, allowing simultaneous detection of molecular properties of cells, is an essential tool for characterizing the complex cellular mechanisms in translational research and clinical practice. New…

The interactions between tumor cells and the tumor microenvironment (TME) dictate therapeutic efficacy of radiation and many systemic therapies in breast cancer. However, to date, there is not a widely available method to reproducibly…

Deep learning-based image fusion approaches have obtained wide attention in recent years, achieving promising performance in terms of visual perception. However, the fusion module in the current deep learning-based methods suffers from two…

Computer Vision and Pattern Recognition · Computer Science 2022-02-01 Dongyu Rao , Xiao-Jun Wu , Tianyang Xu , Guoyang Chen

Accurate diagnosis of breast cancer in histopathology images is challenging due to the heterogeneity of cancer cell growth as well as of a variety of benign breast tissue proliferative lesions. In this paper, we propose a practical and…

Computer Vision and Pattern Recognition · Computer Science 2020-05-06 Xingyu Li , Marko Radulovic , Ksenija Kanjer , Konstantinos N. Plataniotis

Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…

Unsupervised Anomaly Detection has become a popular method to detect pathologies in medical images as it does not require supervision or labels for training. Most commonly, the anomaly detection model generates a "normal" version of an…

Image and Video Processing · Electrical Eng. & Systems 2023-09-26 Felix Meissen , Johannes Paetzold , Georgios Kaissis , Daniel Rueckert

Protein function is inherently linked to its localization within the cell, and fluorescent microscopy data is an indispensable resource for learning representations of proteins. Despite major developments in molecular representation…

Quantitative Methods · Quantitative Biology 2022-05-25 Anastasia Razdaibiedina , Alexander Brechalov

Cancer is one of the leading causes of death worldwide, making the development of rapid, minimally invasive, label-free and scalable diagnostic strategies a major challenge in modern oncology. In this context, spectroscopic liquid biopsy…

Patient-derived extracellular vesicles (EVs) that contains a complex biological cargo is a valuable source of liquid biopsy diagnostics to aid in early detection, cancer screening, and precision nanotherapeutics. In this study, we predicted…

We present a method for automatic cell classification in tissue samples using an automated training set from multiplexed immunofluorescence images. The method utilizes multiple markers stained in situ on a single tissue section on a robust…

Image and Video Processing · Electrical Eng. & Systems 2020-07-21 Alberto Santamaria-Pang , Anup Sood , Dan Meyer , Aritra Chowdhury , Fiona Ginty

Breast cancer is one of the most common types of cancer and leading cancer-related death causes for women. In the context of ICIAR 2018 Grand Challenge on Breast Cancer Histology Images, we compare one handcrafted feature extractor and five…

Computer Vision and Pattern Recognition · Computer Science 2018-04-02 Hongliu Cao , Simon Bernard , Laurent Heutte , Robert Sabourin

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Medical images can be a valuable resource for reliable information to support medical diagnosis. However, the large volume of medical images makes it challenging to retrieve relevant information given a particular scenario. To solve this…

Computer Vision and Pattern Recognition · Computer Science 2016-10-04 S. Sharma , I. Umar , L. Ospina , D. Wong , H. R. Tizhoosh

We evaluate the performance of federated learning (FL) in developing deep learning models for analysis of digitized tissue sections. A classification application was considered as the example use case, on quantifiying the distribution of…

Image and Video Processing · Electrical Eng. & Systems 2022-04-04 Ujjwal Baid , Sarthak Pati , Tahsin M. Kurc , Rajarsi Gupta , Erich Bremer , Shahira Abousamra , Siddhesh P. Thakur , Joel H. Saltz , Spyridon Bakas

Recent advancements in graph-based approaches for multiplexed immunofluorescence (mIF) images have significantly propelled the field forward, offering deeper insights into patient-level phenotyping. However, current graph-based…

Computer Vision and Pattern Recognition · Computer Science 2024-07-26 Sukwon Yun , Jie Peng , Alexandro E. Trevino , Chanyoung Park , Tianlong Chen