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Advances in spatial transcriptomics (ST) technologies enable systematic molecular characterization of tumor microenvironment, tumor gradients and gene regulatory networks. Cancer progression is known to vary along pathological gradients,…

Spatially Resolved Transcriptomics (SRT) is a cutting-edge technique that captures the spatial context of cells within tissues, enabling the study of complex biological networks. Recent graph-based methods leverage both gene expression and…

Machine Learning · Computer Science 2025-06-24 Yunhak Oh , Junseok Lee , Yeongmin Kim , Sangwoo Seo , Namkyeong Lee , Chanyoung Park

Recent advancements in spatial transcriptomics technologies allow researchers to simultaneously measure RNA expression levels for hundreds to thousands of genes while preserving spatial information within tissues, providing critical…

Methodology · Statistics 2025-07-31 Catherine Higgins , Jingyi Jessica Li , Michelle Carey

Spatially resolved transcriptomics (ST) measures gene expression along with the spatial coordinates of the measurements. The analysis of ST data involves significant computation complexity. In this work, we propose gene expression…

Genomics · Quantitative Biology 2022-05-24 Zhuoyan Xu , Kris Sankaran

Identifying genes that display spatial patterns is critical to investigating expression interactions within a spatial context and further dissecting biological understanding of complex mechanistic functionality. Despite the increase in…

Methodology · Statistics 2025-10-06 Mingcong Wu , Yang Li , Shuangge Ma , Mengyun Wu

The rapid development of spatial transcriptomics (ST) offers new opportunities to explore the gene expression patterns within the spatial microenvironment. Current research integrates pathological images to infer gene expression, addressing…

Computer Vision and Pattern Recognition · Computer Science 2025-03-03 Junchao Zhu , Ruining Deng , Tianyuan Yao , Juming Xiong , Chongyu Qu , Junlin Guo , Siqi Lu , Yucheng Tang , Daguang Xu , Mengmeng Yin , Yu Wang , Shilin Zhao , Yaohong Wang , Haichun Yang , Yuankai Huo

Spatial transcriptomics (ST) enables gene expression mapping within anatomical context but remains costly and low-throughput. Hematoxylin and eosin (H\&E) staining offers rich morphology yet lacks molecular resolution. We present…

Spatially resolved transcriptomics (SRT) has evolved rapidly through various technologies, enabling scientists to investigate both morphological contexts and gene expression profiling at single-cell resolution in parallel. SRT data are…

Spatial Transcriptomics (ST) profiles thousands of gene expression values at discrete spots with precise coordinates on tissue sections, preserving spatial context essential for clinical and pathological studies. With rising sequencing…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Yishun Zhu , Jiaxin Qi , Jian Wang , Yuhua Zheng , Jianqiang Huang

Spatial transcriptomics is an emerging technology that aligns histopathology images with spatially resolved gene expression profiling. It holds the potential for understanding many diseases but faces significant bottlenecks such as…

Computer Vision and Pattern Recognition · Computer Science 2024-01-12 Gabriel Mejia , Paula Cárdenas , Daniela Ruiz , Angela Castillo , Pablo Arbeláez

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Spatial transcriptomics (ST) technologies can be used to align transcriptomes with histopathological morphology, presenting exciting new opportunities for biomolecular discovery. Using ST data, we construct a novel framework, GeneFlow, to…

Quantitative Methods · Quantitative Biology 2025-11-04 Mengbo Wang , Shourya Verma , Aditya Malusare , Luopin Wang , Yiyang Lu , Vaneet Aggarwal , Mario Sola , Ananth Grama , Nadia Atallah Lanman

Spatial Transcriptomics (ST) offers spatially resolved gene expression but remains costly. Predicting expression directly from widely available Hematoxylin and Eosin (H&E) stained images presents a cost-effective alternative. However, most…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Jiarui Ouyang , Yihui Wang , Yihang Gao , Yingxue Xu , Shu Yang , Hao Chen

Spatial transcriptomics is a modern sequencing technology that allows the measurement of the activity of thousands of genes in a tissue sample and map where the activity is occurring. This technology has enabled the study of the so-called…

Methodology · Statistics 2022-09-15 Andrea Sottosanti , Davide Risso

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-01-25 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

When a high-resolution (HR) image is degraded into a low-resolution (LR) image, the image loses some of the existing information. Consequently, multiple HR images can correspond to the LR image. Most of the existing methods do not consider…

Computer Vision and Pattern Recognition · Computer Science 2022-07-19 Hanbyel Cho , Yekang Lee , Jaemyung Yu , Junmo Kim

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-12-23 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Spatial transcriptomics (ST) enables mapping gene expression with spatial context but is severely affected by high sparsity and technical noise, which conceals true biological signals and hinders downstream analyses. To address these…

Machine Learning · Computer Science 2026-03-10 Sayeem Bin Zaman , Fahim Hafiz , Riasat Azim

Spatial transcriptomics (ST) bridges gene expression and tissue morphology but faces clinical adoption barriers due to technical complexity and prohibitive costs. While computational methods predict gene expression from H&E-stained…

Computer Vision and Pattern Recognition · Computer Science 2025-11-20 Ziqiao Weng , Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee AD Cooper , Weidong Cai , Bo Zhou

Spatial transcriptomics studies are becoming increasingly large and commonplace, necessitating simultaneous analysis of a large number of spatially resolved variables. Correspondingly, a diverse range of methodologies have been proposed to…

Quantitative Methods · Quantitative Biology 2025-09-09 James Boyle , Gregory Hamm , Eleanor Williams , Robin JG Hartman , Magnus Soderburg , Ian Henry , Michael Casey