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Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

In the process of performing image super-resolution processing, the processing of complex localized information can have a significant impact on the quality of the image generated. Fractal features can capture the rich details of both micro…

Computer Vision and Pattern Recognition · Computer Science 2025-02-28 Lianping Yang , Peng Jiao , Jinshan Pan , Hegui Zhu , Su Guo

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Single image super-resolution (SISR), which aims to reconstruct a high-resolution (HR) image from a low-resolution (LR) observation, has been an active research topic in the area of image processing in recent decades. Particularly, deep…

Image and Video Processing · Electrical Eng. & Systems 2021-03-04 Honggang Chen , Xiaohai He , Linbo Qing , Yuanyuan Wu , Chao Ren , Ce Zhu

In fluorescence microscopy live-cell imaging, there is a critical trade-off between the signal-to-noise ratio and spatial resolution on one side, and the integrity of the biological sample on the other side. To obtain clean high-resolution…

Image and Video Processing · Electrical Eng. & Systems 2020-08-25 Ruofan Zhou , Majed El Helou , Daniel Sage , Thierry Laroche , Arne Seitz , Sabine Süsstrunk

The objective of image super-resolution is to reconstruct a high-resolution (HR) image with the prior knowledge from one or several low-resolution (LR) images. However, in the real world, due to the limited complementary information, the…

Image and Video Processing · Electrical Eng. & Systems 2024-12-16 Jing Sun , Qiangqiang Yuan , Huanfeng Shen , Jie Li , Liangpei Zhang

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

While super-resolution (SR) methods based on diffusion models exhibit promising results, their practical application is hindered by the substantial number of required inference steps. Recent methods utilize degraded images in the initial…

Computer Vision and Pattern Recognition · Computer Science 2023-11-28 Yufei Wang , Wenhan Yang , Xinyuan Chen , Yaohui Wang , Lanqing Guo , Lap-Pui Chau , Ziwei Liu , Yu Qiao , Alex C. Kot , Bihan Wen

Single image super-resolution (SISR) is a notoriously challenging ill-posed problem, which aims to obtain a high-resolution (HR) output from one of its low-resolution (LR) versions. To solve the SISR problem, recently powerful deep learning…

Computer Vision and Pattern Recognition · Computer Science 2019-07-15 Wenming Yang , Xuechen Zhang , Yapeng Tian , Wei Wang , Jing-Hao Xue

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Fluorescence microscopy is a key driver to promote discoveries of biomedical research. However, with the limitation of microscope hardware and characteristics of the observed samples, the fluorescence microscopy images are susceptible to…

Image and Video Processing · Electrical Eng. & Systems 2022-09-15 Xuanyu Tian , Qing Wu , Hongjiang Wei , Yuyao Zhang

We propose a simple yet effective model for Single Image Super-Resolution (SISR), by combining the merits of Residual Learning and Convolutional Sparse Coding (RL-CSC). Our model is inspired by the Learned Iterative Shrinkage-Threshold…

Computer Vision and Pattern Recognition · Computer Science 2019-01-01 Menglei Zhang , Zhou Liu , Lei Yu

Single image super-resolution (SISR) is the process of obtaining one high-resolution version of a low-resolution image by increasing the number of pixels per unit area. This method has been actively investigated by the research community,…

Image and Video Processing · Electrical Eng. & Systems 2022-10-11 George Corrêa de Araújo , Helio Pedrini

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…

Recent progress in single-image super-resolution (SISR) has achieved remarkable performance, yet the computational costs of these methods remain a challenge for deployment on resource-constrained devices. In particular, transformer-based…

Computer Vision and Pattern Recognition · Computer Science 2024-10-22 Gang Wu , Junjun Jiang , Junpeng Jiang , Xianming Liu

Confocal microscopy is the standard approach for obtaining volumetric images of a sample with high axial and lateral resolution, especially when dealing with scattering samples. Unfortunately, a confocal microscope is quite expensive…

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