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Spatially resolved transcriptomics represents a significant advancement in single-cell analysis by offering both gene expression data and their corresponding physical locations. However, this high degree of spatial resolution entails a…

Genomics · Quantitative Biology 2024-03-19 Xiaoyu Li , Wenwen Min , Shunfang Wang , Changmiao Wang , Taosheng Xu

Spatial Transcriptomics (ST) reveals the spatial distribution of gene expression in tissues, offering critical insights into biological processes and disease mechanisms. However, the high cost, limited coverage, and technical complexity of…

Computer Vision and Pattern Recognition · Computer Science 2025-04-22 Yi Niu , Jiashuai Liu , Yingkang Zhan , Jiangbo Shi , Di Zhang , Marika Reinius , Ines Machado , Mireia Crispin-Ortuzar , Jialun Wu , Chen Li , Zeyu Gao

High-resolution spatial transcriptomics platforms, such as Xenium, generate single-cell images that capture both molecular and spatial context, but their extremely high dimensionality poses major challenges for representation learning and…

The rapid development of spatial transcriptomics (ST) offers new opportunities to explore the gene expression patterns within the spatial microenvironment. Current research integrates pathological images to infer gene expression, addressing…

Computer Vision and Pattern Recognition · Computer Science 2025-03-03 Junchao Zhu , Ruining Deng , Tianyuan Yao , Juming Xiong , Chongyu Qu , Junlin Guo , Siqi Lu , Yucheng Tang , Daguang Xu , Mengmeng Yin , Yu Wang , Shilin Zhao , Yaohong Wang , Haichun Yang , Yuankai Huo

Single-cell transcriptomics and proteomics have become a great source for data-driven insights into biology, enabling the use of advanced deep learning methods to understand cellular heterogeneity and gene expression at the single-cell…

Genomics · Quantitative Biology 2025-12-15 Hiren Madhu , João Felipe Rocha , Tinglin Huang , Siddharth Viswanath , Smita Krishnaswamy , Rex Ying

Histopathology image classification is crucial for the accurate identification and diagnosis of various diseases but requires large and diverse datasets. Obtaining such datasets, however, is often costly and time-consuming due to the need…

Computer Vision and Pattern Recognition · Computer Science 2024-09-25 Leire Benito-Del-Valle , Aitor Alvarez-Gila , Itziar Eguskiza , Cristina L. Saratxaga

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Spatial Transcriptomics (ST) provides spatially resolved gene expression profiles within intact tissue architecture, enabling molecular analysis in histological context. However, the high cost, limited throughput, and restricted data…

Machine Learning · Computer Science 2026-03-31 Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee A. Cooper , Bo Zhou

Recent developments in spatial omics technologies have enabled the generation of high dimensional molecular data, such as transcriptomes, proteomes, and epigenomes, within their spatial tissue context, either through coprofiling on the same…

Quantitative Methods · Quantitative Biology 2026-01-21 Esra Busra Isik , Yusuf Hakan Usta , Haozhe Liu , Maryam Riazi , William Roach , Hongpeng Zhou , Magnus Rattray , Sokratia Georgaka

High-resolution spatial transcriptomics (HR-ST) technologies offer unprecedented insights into tissue architecture but lack standardized frameworks for histological annotation. We present ST2HE, a cross-platform generative framework that…

Quantitative Methods · Quantitative Biology 2025-10-16 Zhentao Liu , Arun Das , Wen Meng , Yu-Chiao Chiu , Shou-Jiang Gao , Yufei Huang

In recent years, a standard computational pathology workflow has emerged where whole slide images are cropped into tiles, these tiles are processed using a foundation model, and task-specific models are built using the resulting…

Computer Vision and Pattern Recognition · Computer Science 2026-02-26 Eric Zimmermann , Julian Viret , Michal Zelechowski , James Brian Hall , Neil Tenenholtz , Adam Casson , George Shaikovski , Eugene Vorontsov , Siqi Liu , Kristen A Severson

Pathology image segmentation is crucial in computational pathology for analyzing histological features relevant to cancer diagnosis and prognosis. However, current methods face major challenges in clinical applications due to limited…

Computer Vision and Pattern Recognition · Computer Science 2025-08-20 Zhixuan Chen , Junlin Hou , Liqi Lin , Yihui Wang , Yequan Bie , Xi Wang , Yanning Zhou , Ronald Cheong Kin Chan , Hao Chen

Spatially resolved transcriptomics (ST) measures gene expression along with the spatial coordinates of the measurements. The analysis of ST data involves significant computation complexity. In this work, we propose gene expression…

Genomics · Quantitative Biology 2022-05-24 Zhuoyan Xu , Kris Sankaran

Medical image segmentation is a critical component in clinical practice, facilitating accurate diagnosis, treatment planning, and disease monitoring. However, existing methods, often tailored to specific modalities or disease types, lack…

Image and Video Processing · Electrical Eng. & Systems 2024-04-02 Jun Ma , Yuting He , Feifei Li , Lin Han , Chenyu You , Bo Wang

Spatial transcriptomics (ST) enables mapping gene expression with spatial context but is severely affected by high sparsity and technical noise, which conceals true biological signals and hinders downstream analyses. To address these…

Machine Learning · Computer Science 2026-03-10 Sayeem Bin Zaman , Fahim Hafiz , Riasat Azim

Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…

Identifying disease-indicative genes is critical for deciphering disease mechanisms and has attracted significant interest in biomedical research. Spatial transcriptomics offers unprecedented insights for the detection of disease-specific…

Methodology · Statistics 2024-09-05 Qicheng Zhao , Qihuang Zhang

Spatial transcriptomics (ST) technologies enable gene expression profiling with spatial resolution, offering unprecedented insights into tissue organization and disease heterogeneity. However, current analysis methods often struggle with…

Spatial transcriptomics (ST) bridges gene expression and tissue morphology but faces clinical adoption barriers due to technical complexity and prohibitive costs. While computational methods predict gene expression from H&E-stained…

Computer Vision and Pattern Recognition · Computer Science 2025-11-20 Ziqiao Weng , Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee AD Cooper , Weidong Cai , Bo Zhou

Spatial transcriptomics (ST) provides crucial insights into tissue micro-environments, but is limited to its high cost and complexity. As an alternative, predicting gene expression from pathology whole slide images (WSI) is gaining…

Computer Vision and Pattern Recognition · Computer Science 2025-07-01 Mingcheng Qu , Yuncong Wu , Donglin Di , Yue Gao , Tonghua Su , Yang Song , Lei Fan