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scRNA-seq clustering is a critical task for analyzing single-cell RNA sequencing (scRNA-seq) data, as it groups cells with similar gene expression profiles. Transformers, as powerful foundational models, have been applied to scRNA-seq…

Machine Learning · Computer Science 2026-02-10 Zhuomin Liang , Liang Bai , Xian Yang

The newly developed deep-sequencing technologies make it possible to acquire both quantitative and qualitative information regarding transcript biology. By measuring messenger RNA levels for all genes in a sample, RNA-seq provides an…

Genomics · Quantitative Biology 2014-12-05 Lerong Li , Momiao Xiong

In line with the importance of RNA-seq, the bioinformatics community has produced numerous data analysis tools incorporating methods to correct sample-specific biases. However, few advanced simulation tools exist to enable benchmarking of…

Genomics · Quantitative Biology 2013-08-15 Botond Sipos , Greg Slodkowicz , Tim Massingham , Nick Goldman

The application of machine learning to transcriptomics data has led to significant advances in cancer research. However, the high dimensionality and complexity of RNA sequencing (RNA-seq) data pose significant challenges in pan-cancer…

Genomics · Quantitative Biology 2024-08-15 Jong Hyun Kim , Jongseong Jang

Modeling cellular dynamics from single-cell RNA sequencing (scRNA-seq) data is critical for understanding cell development and underlying gene regulatory relationships. Many current methods rely on single-cell velocity to obtain pseudotime,…

Molecular Networks · Quantitative Biology 2024-10-25 Zhen Zhou , Jiachen Li , Hongyi Xin , Xiaoyong Pan , Hong-Bin Shen

Survival analysis consists of studying the elapsed time until an event of interest, such as the death or recovery of a patient in medical studies. This work explores the potential of neural networks in survival analysis from clinical and…

Statistics Theory · Mathematics 2021-05-19 Mathilde Sautreuil , Sarah Lemler , Paul-Henry Cournède

Induced pluripotent stem cells (iPSCs) provide a great model to study the process of reprogramming and differentiation of stem cells. Single-cell RNA sequencing (scRNA-seq) enables us to investigate the reprogramming process at single-cell…

Cell Behavior · Quantitative Biology 2020-12-30 Yusong Ye , Zhuoqin Yang , Jinzhi Lei

Recent advances in high-throughput cDNA sequencing (RNA-Seq) technology have revolutionized transcriptome studies. A major motivation for RNA-Seq is to map the structure of expressed transcripts at nucleotide resolution. With accurate…

Genomics · Quantitative Biology 2013-09-23 Georg Zeller , Nico Goernitz , Andre Kahles , Jonas Behr , Pramod Mudrakarta , Soeren Sonnenburg , Gunnar Raetsch

An important challenge in cancer systems biology is to uncover the complex network of interactions between genes (tumor suppressor genes and oncogenes) implicated in cancer. Next generation sequencing provides unparalleled ability to probe…

Genomics · Quantitative Biology 2012-12-10 Ying Cai , Bernard Fendler , Gurinder S. Atwal

By combining various cancer cell line (CCL) drug screening panels, the size of the data has grown significantly to begin understanding how advances in deep learning can advance drug response predictions. In this paper we train >35,000…

Single-cell RNA-seq provides detailed molecular snapshots of individual cells but is notoriously noisy. Variability stems from biological differences and technical factors, such as amplification bias and limited RNA capture efficiency,…

Machine Learning · Computer Science 2026-02-27 Victor Chardès

RNAs are essential molecules that carry genetic information vital for life, with profound implications for drug development and biotechnology. Despite this importance, RNA research is often hindered by the vast literature available on the…

Genomics · Quantitative Biology 2024-11-15 Yijia Xiao , Edward Sun , Yiqiao Jin , Wei Wang

The rise of single-cell sequencing technologies has revolutionized the exploration of drug resistance, revealing the crucial role of cellular heterogeneity in advancing precision medicine. By building computational models from existing…

Genomics · Quantitative Biology 2025-02-05 Yu-An Huang , Xiyue Cao , Zhu-Hong You , Yue-Chao Li , Xuequn Shang , Zhi-An Huang

This issue includes six articles that develop and apply statistical methods for the analysis of gene sequencing data of different types. The methods are tailored to the different data types and, in each case, lead to biological insights not…

Applications · Statistics 2012-06-29 Karen Kafadar

Ultra high-throughput sequencing of transcriptomes (RNA-Seq) is a widely used method for quantifying gene expression levels due to its low cost, high accuracy and wide dynamic range for detection. However, the nature of RNA-Seq makes it…

Methodology · Statistics 2016-08-30 Hui Jiang , Tianyu Zhan

Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cells without sample pooling or RNA extraction. Currently, there is no algorithm optimized…

In a previous paper [CSIAM Trans. Appl. Math. 2 (2021), 1-55], the authors proposed a theoretical framework for the analysis of RNA velocity, which is a promising concept in scRNA-seq data analysis to reveal the cell state-transition…

Numerical Analysis · Mathematics 2025-02-25 Tiejun Li , Yizhuo Wang , Guoguo Yang , Peijie Zhou

Although bulk transcriptomic analyses have significantly contributed to an enhanced comprehension of multifaceted diseases, their exploration capacity is impeded by the heterogeneous compositions of biological samples. Indeed, by averaging…

Quantitative Methods · Quantitative Biology 2023-10-24 Bastien Chassagnol , Grégory Nuel , Etienne Becht

In the analysis of single-cell RNA sequencing data, researchers often characterize the variation between cells by estimating a latent variable, such as cell type or pseudotime, representing some aspect of the individual cell's state. They…

Methodology · Statistics 2022-10-19 Anna Neufeld , Lucy L. Gao , Joshua Popp , Alexis Battle , Daniela Witten

Genome annotation is an important issue in biology which has long been addressed with gene prediction methods and manual experiments requiring biological expertise. The expanding Next Generation Sequencing technologies and their enhanced…

Computation · Statistics 2013-07-02 Alice Cleynen , Michel Koskas , Emilie Lebarbier , Guillem Rigaill , Stephane Robin