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Digital holography (DH) enables non-contact, noninvasive 3D imaging of transparent and moving microscopic samples by capturing amplitude and phase information in a single shot. In this work, we present a compact, low-cost, real-time…

Optics · Physics 2025-03-18 Yuki Nagahama

Microscopic analysis of micro particles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require…

Robotics · Computer Science 2019-11-26 Kevin Mallery , Dario Canelon , Jiarong Hong , Nikolaos Papanikolopoulos

In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…

Quantitative Methods · Quantitative Biology 2018-04-06 Mirjam Schürmann , Jana Scholze , Paul Müller , Chii J. Chan , Andrew E. Ekpenyong , Kevin J. Chalut , Jochen Guck

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

We present a new digital holographic microscope (DHM) with a low coherent source for the quantitative imaging of smooth and optically rough objects. The experimental design of the microscope uses an in-line experimental geometry based on…

This study developed handheld and low-cost digital holographic microscopy (DHM) by adopting an in-line type hologram, a webcam, a high power RGB light emitting diode (LED), and a pinhole. It cost less than 20,000 yen (approximately 250 US…

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Hologram is an ideal method for naked eye three-dimensional (3D) display, and computer-generated holography (CGH) makes it possible to reconstruct virtual objects. However, the large pixel size of common CGH devices results in shortages in…

Optics · Physics 2019-09-13 Hui Gao , Yuxi Wang , Xuhao Fan , Fenzhang Jiao , Jinsong Xia , Wei Xiong , Minghui Hong

In this paper, we introduce Holographic Spectral Multiplexing (HSM) as a novel technique to enable multiple-input multiple-output (MIMO) communication in optical networks. HSM uses the spectral space of ultrashort laser pulses to create…

Networking and Internet Architecture · Computer Science 2022-02-03 Alireza Khodaei , Jitender Deogun

Expensive scientific camera hardware is amongst the main cost factors in modern, high-performance microscopes. Recent technological advantages have, however, yielded consumer-grade camera devices that can provide surprisingly good…

Image and Video Processing · Electrical Eng. & Systems 2019-03-06 Benedict Diederich , Patrick Then , Alexander Jügler , Ronny Förster , Rainer Heintzmann

Computer-generated holography (CGH) can be used to display three-dimensional (3D) images and has a special feature that no other technology possesses: it can reconstruct arbitrary object wavefronts. In this study, we investigated a…

Optics · Physics 2023-10-17 Shuhei Yoshida

The outbreak of COVID-19 exposed the inadequacy of our technical tools for home health surveillance, and recent studies have shown the potential of smartphones as a universal optical microscopic imaging platform for such applications.…

Image and Video Processing · Electrical Eng. & Systems 2023-12-20 Haoran Zhang , Weiyi Zhang , Zirui Zuo , Jianlong Yang

Close to half of the world population have smartphones, while a typical flagship smartphone today has been integrated with more than 20 smart components and sensors, making a smartphone a highly integrated platform that can potentially…

Image and Video Processing · Electrical Eng. & Systems 2020-10-21 Jinlong Zhu , Ni Zhao , Renjie Zhou

3D photography is a new medium that allows viewers to more fully experience a captured moment. In this work, we refer to a 3D photo as one that displays parallax induced by moving the viewpoint (as opposed to a stereo pair with a fixed…

Digital holography (DH) has been widely used for imaging and characterization of micro and nanostructures in materials science and biology and has the potential to provide high-resolution, non-destructive measurements of fluid surfaces as…

This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…

Optics · Physics 2007-05-23 Sergey A. Alexandrov , P. Meredith , T. J. McIntyre , A. V. Zvyagin

Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…

Optics · Physics 2019-04-10 Stephan Amann , Max von Witzleben , and Stefan Breuer

Digital holography is an interference-based imaging technique capable of recording both the amplitude and phase of an electromagnetic field. It can be obtained at the laser illumination wavelength, but also with second-harmonic generation,…

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