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Liquid-liquid phase separation of aqueous two-phase system (ATPS) is fundamental across physical and biological sciences. While well understood for passive systems, how this process is regulated by active agents such as motile bacteria…

Soft Condensed Matter · Physics 2025-11-25 Dixi Yang , Anheng Wang , Chunming Wang , Hajime Tanaka , Jiaxing Yuan

Real-life bacteria often swim in complex fluids, but our understanding of the interactions between bacteria and complex surroundings is still evolving. In this work, rod-like \textit{Bacillus subtilis} swims in a quasi-2D environment with…

Proliferation and motility are ubiquitous drivers of activity in biological systems. Here, we study a dense binary mixture of motile and proliferating particles with exclusively repulsive interactions, where homeostasis in the proliferating…

Soft Condensed Matter · Physics 2025-06-11 Lukas Hupe , Joanna M. Materska , David Zwicker , Ramin Golestanian , Bartlomiej Waclaw , Philip Bittihn

Mixtures of polyethylene glycol (PEG) and dextran (Dex) represent a widely used class of aqueous two-phase systems (ATPS), with applications ranging from the purification of various biomolecules such as nucleic acids to the synthesis of…

Soft Condensed Matter · Physics 2026-01-28 Hiroki Sakuta , Yuki Akamine , Akari Kamo , Hao Gong , Norikazu Ichihashi , Arash Nikoubashman , Miho Yanagisawa

We study the transport of bacteria in a porous media modeled by a square channel containing one cylindrical obstacle via molecular dynamics simulations coupled to a lattice Boltzmann fluid. Our bacteria model is a rod-shaped rigid body…

Soft Condensed Matter · Physics 2020-11-30 Miru Lee , Christoph Lohrmann , Kai Szuttor , Harold Auradou , Christian Holm

Understanding flow and transport of bacteria in porous media is crucial to technologies such as bioremediation, biomineralization or enhanced oil recovery. While physicochemical bacteria filtration is well-documented, recent studies showed…

Soft Condensed Matter · Physics 2022-08-24 Marco Dentz , Adama Creppy , Carine Douarche , Eric Clément , Harold Auradou

The rapid adhesion of motile bacteria from dilute suspensions poses a fundamental non-equilibrium problem: hydrodynamic interactions bias bacterial motion near surfaces without generating stable confinement, while electrostatic interactions…

Soft Condensed Matter · Physics 2026-01-13 Dixi Yang , Anheng Wang , Jia Huang , Xiaofeng Zhuo , Chunming Wang , Hajime Tanaka , Jiaxing Yuan

Liquid-liquid phase separation is important across biology, physics, and materials science. Although usually studied at equilibrium, active components - such as motor proteins, enzymes, and synthetic microswimmers - are increasingly…

Soft Condensed Matter · Physics 2026-01-06 Kan Chang , Yulin Li , Ming Yuan , Masaki Sano , Zhihong You , H. P. Zhang

Densely packed, motile bacteria can adopt collective states not seen in conventional, passive materials. These states remain in many ways mysterious, and their physical characterization can aid our understanding of natural bacterial…

Statistical Mechanics · Physics 2024-06-18 Hisay Lama , Masahiro J. Yamamoto , Yujiro Furuta , Takuro Shimaya , Kazumasa A. Takeuchi

Bacterial migration through confined spaces is critical for several phenomena like: biofilm formation, bacterial transport in soils, and bacterial therapy against cancer . In the present work, \textit{E. coli} (strain K12-MG1655 WT)…

The motility of a fish keratocyte on a flat substrate exhibits two distinct regimes: the non-migrating and the migrating one. In both configurations the shape is fixed in time and, when the cell is moving, the velocity is constant in…

Cell Behavior · Quantitative Biology 2016-06-29 Davide Ambrosi , Anna Zanzottera

Using a multi-phase field model, we examine how particle deformability, which is a proxy for cell stiffness, affects motility induced phase separation (MIPS). We show that purely repulsive deformable, i.e., squishy, cells phase separate…

Soft Condensed Matter · Physics 2025-02-12 Austin Hopkins , Benjamin Loewe , Michael Chiang , Davide Marenduzzo , M. Cristina Marchetti

Liquid-liquid phase separation is emerging as a crucial phenomenon in several fundamental cell processes. A range of eukaryotic systems exhibit liquid condensates. However, their function in bacteria, which in general lack membrane-bound…

We experimentally investigate how aerotactic bacteria, confined within a thin liquid film between two solid substrates, respond to a controlled oxygen gradient. We find that the total bacterial number density dictates which mechanism…

Soft Condensed Matter · Physics 2026-04-07 Minjun Kim , Joonwoo Jeong

How systems are endowed with migration capacity is a fascinating question with implications ranging from the design of novel active systems to the control of microbial populations. Bacteria, which can be found in a variety of environments,…

Biological Physics · Physics 2017-07-03 Marc Hennes , Julien Tailleur , Gaëlle Charron , Adrian Daerr

While bacterial swimming has been well characterized in uniform liquid environments, only little is known about how bacteria propagate through complex environments, such as gel-like matrices or porous media that are typically encountered in…

Soft Condensed Matter · Physics 2024-08-06 Agniva Datta , Sönke Beier , Veronika Pfeifer , Robert Großmann , Carsten Beta

How motile bacteria move near a surface is a problem of fundamental biophysical interest and is key to the emergence of several phenomena of biological, ecological and medical relevance, including biofilm formation. Solid boundaries can…

Biological Physics · Physics 2019-09-12 Stanislaw Makarchuk , Vasco C. Braz , Nuno A. M. Araújo , Lena Ciric , Giorgio Volpe

Bacteria can spontaneously develop collective motions by aligning their motions in dense systems. Here, we show that bacteria can also respond collectively to an alternating electrical field and form dynamic clusters oscillating at the same…

Soft Condensed Matter · Physics 2022-10-11 Mei Mei Bao , Isaiah Eze Igwe , Kang Chen , Tian Hui Zhang

The role of activity on the hydrodynamic dispersion of bacteria in a model porous medium is studied by tracking thousands of bacteria in a microfluidic chip containing randomly placed pillars. We first evaluate the spreading dynamics of two…

We study colonies of non-motile, rod-shaped bacteria growing on solid substrates. In our model, bacteria interact purely mechanically, by pushing each other away as they grow, and consume a diffusing nutrient. We show that mechanical…

Biological Physics · Physics 2015-06-15 F. D. C. Farrell , O. Hallatschek , D. Marenduzzo , B. Waclaw
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