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Automated cell segmentation is crucial for various biological and medical applications, facilitating tasks like cell counting, morphology analysis, and drug discovery. However, manual segmentation is time-consuming and prone to…

Machine Learning · Computer Science 2024-09-13 Aaron Rock Menezes , Bharath Ramsundar

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Current biological and medical research is aimed at obtaining a detailed spatiotemporal map of a live cell's interior to describe and predict cell's physiological state. We present here an algorithm for complete 3-D modelling of cellular…

Quantitative Methods · Quantitative Biology 2017-04-03 Renata Rychtarikova , Tomas Nahlik , Kevin Shi , Daria Malakhova , Petr Machacek , Rebecca Smaha , Jan Urban , Dalibor Stys

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Advances in image registration and machine learning have recently enabled volumetric analysis of postmortem brain tissue from conventional photographs of coronal slabs, which are routinely collected in brain banks and neuropathology…

Wood comprises different cell types, such as fibers, tracheids and vessels, defining its properties. Studying cells' shape, size, and arrangement in microscopy images is crucial for understanding wood characteristics. Typically, this…

Computer Vision and Pattern Recognition · Computer Science 2024-06-19 Saqib Qamar , Abu Imran Baba , Stéphane Verger , Magnus Andersson

On-line segmentation of the uterus can aid effective image-based guidance for precise delivery of dose to the target tissue (the uterocervix) during cervix cancer radiotherapy. 3D ultrasound (US) can be used to image the uterus, however,…

Image and Video Processing · Electrical Eng. & Systems 2021-09-21 Bahareh Behboodi , Hassan Rivaz , Susan Lalondrelle , Emma Harris

This paper presents a method for time-lapse 3D cell analysis. Specifically, we consider the problem of accurately localizing and quantitatively analyzing sub-cellular features, and for tracking individual cells from time-lapse 3D confocal…

Computer Vision and Pattern Recognition · Computer Science 2022-08-18 Jiaxiang Jiang , Amil Khan , S. Shailja , Samuel A. Belteton , Michael Goebel , Daniel B. Szymanski , B. S. Manjunath

Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

Computer Vision and Pattern Recognition · Computer Science 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

We show dense voxel embeddings learned via deep metric learning can be employed to produce a highly accurate segmentation of neurons from 3D electron microscopy images. A "metric graph" on a set of edges between voxels is constructed from…

Computer Vision and Pattern Recognition · Computer Science 2021-08-09 Kisuk Lee , Ran Lu , Kyle Luther , H. Sebastian Seung

We present a dual-stage neural network architecture for analyzing fine shape details from microscopy recordings in 3D. The system, tested on red blood cells, uses training data from both healthy donors and patients with a congenital blood…

Quantitative Methods · Quantitative Biology 2020-05-29 G. Simionato , K. Hinkelmann , R. Chachanidze , P. Bianchi , E. Fermo , R. van Wijk , M. Leonetti , C. Wagner , L. Kaestner , S. Quint

Cell segmentation is a major bottleneck in extracting quantitative single-cell information from microscopy data. The challenge is exasperated in the setting of microstructured environments. While deep learning approaches have proven useful…

Quantitative Methods · Quantitative Biology 2021-01-08 Tim Prangemeier , Christian Wildner , André O. Françani , Christoph Reich , Heinz Koeppl

Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an…

Image and Video Processing · Electrical Eng. & Systems 2025-05-06 Shuang Zhang , Carleton Coffin , Karyn L. Rogers , Catherine Ann Royer , Ge Wang

Three-dimensional volumetric imaging of cells allows for in situ visualization, thus preserving contextual insights into cellular processes. Despite recent advances in machine learning methods, morphological analysis of sub-nuclear…

Quantitative Methods · Quantitative Biology 2022-07-21 Niraj Gupta , Eric J. Roberts , Song Pang , C. Shan Xu , Harald F. Hess , Fan Wu , Abby Dernburg , Danielle Jorgens , Petrus H. Zwart , Vignesh Kasinath

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Microscopy imaging techniques are instrumental for characterization and analysis of biological structures. As these techniques typically render 3D visualization of cells by stacking 2D projections, issues such as out-of-plane excitation and…

Image and Video Processing · Electrical Eng. & Systems 2023-02-16 Amirkoushyar Ziabari , Derek C. Rose , Abbas Shirinifard , David Solecki

One of the most common tasks in medical imaging is semantic segmentation. Achieving this segmentation automatically has been an active area of research, but the task has been proven very challenging due to the large variation of anatomy…

Computer Vision and Pattern Recognition · Computer Science 2018-04-10 Holger R. Roth , Chen Shen , Hirohisa Oda , Masahiro Oda , Yuichiro Hayashi , Kazunari Misawa , Kensaku Mori

The throughput of electron microscopes has increased significantly in recent years, enabling detailed analysis of cell morphology and ultrastructure. Analysis of neural circuits at single-synapse resolution remains the flagship target of…

Computer Vision and Pattern Recognition · Computer Science 2019-08-07 Constantin Pape , Alex Matskevych , Adrian Wolny , Julian Hennies , Giula Mizzon , Marion Louveaux , Jacob Musser , Alexis Maizel , Detlev Arendt , Anna Kreshuk

Motivation: Cellular Electron CryoTomography (CECT) enables 3D visualization of cellular organization at near-native state and in sub-molecular resolution, making it a powerful tool for analyzing structures of macromolecular complexes and…

Quantitative Methods · Quantitative Biology 2017-04-14 Min Xu , Xiaoqi Chai , Hariank Muthakana , Xiaodan Liang , Ge Yang , Tzviya Zeev-Ben-Mordehai , Eric Xing