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Coherent anti-Stokes Raman scattering (CARS) microspectroscopy has demonstrated significant potential for biological and materials imaging. To date, however, the primary mechanism of disseminating CARS spectroscopic information is through…
We present a new interferometric technique for measuring Coherent Anti-Stokes Raman Scattering (CARS) and Second Harmonic Generation (SHG) signals. Heterodyne detection is employed to increase the sensitivity in both CARS and SHG signal…
Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. CARS microscopy can detect lipid…
We extend image scanning microscopy to second harmonic generation (SHG) by extracting the complex field amplitude of the second-harmonic beam. While the theory behind coherent image scanning microscopy (ISM) is known, an experimental…
Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…
The sensitivity of classical Raman spectroscopy methods, such as Coherent Anti-Stokes Raman spectroscopy (CARS) or Stimulated Raman spectroscopy (SRS), is ultimately limited by shot-noise from the stimulating fields. We present the complete…
Stimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS…
Label-free approaches are attractive in cytological imaging due to their flexibility and cost efficiency. They are supported by machine learning methods, which, despite the lack of labeling and the associated lower contrast, can classify…
We investigate how feature selection algorithms can enable accurate, reference-free classification of materials using sparse-frequency terahertz (THz) reflection spectroscopy. Three classes of feature selection strategies are evaluated.…
Coherent spin resonance techniques, such as nuclear and electron spin resonance spectroscopy, have revolutionized non-invasive imaging by providing spectrally resolved information about spin dynamics. Motivated by the recent emergence of…
Coherent Raman scattering microscopy is a fast, label-free and chemically specific imaging technique that has a high potential for future in-vivo optical histology. However, its imaging depth into tissues is limited to the sub-millimeter…
Can a single label-free image reveal whether cancer cells were exposed to chemotherapy? We present an innovative methodology on the label-free and high-resolution imaging properties of phase holotomographic microscopy coupled with neural…
We propose a novel approach using Multiplex-Coherent Anti-Stokes Raman Scattering (M-CARS) for la-bel-free discriminations in biomedical tissues. The strategy is based on the evaluation of the contrast be-tween resonant and nonresonant…
Second-harmonic generation (SHG) microscopy provides label-free imaging of biological tissues with unique contrast mechanisms, but its resolution is limited by the diffraction limit. Here, we present the first experimental demonstration of…
In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destruct the sample or introduce strong perturbations to the functions of…
Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…
Label-free optical microscopy through absorption or scattering spectroscopy provides fundamental insights across biology and materials science, yet its sensitivity remains fundamentally limited by photon shot noise. While recent…
We theoretically and experimentally demonstrate a multifrequency excitation and detection scheme in apertureless near field optical microscopy, that exceeds current state of the art sensitivity and background suppression. By exciting the…
Label-free vibrational imaging by stimulated Raman scattering (SRS) provides unprecedented insight into real-time chemical distributions in living systems. Specifically, SRS in the fingerprint region can resolve multiple chemicals in a…
Recognizing multiple labels of images is a practical and challenging task, and significant progress has been made by searching semantic-aware regions and modeling label dependency. However, current methods cannot locate the semantic regions…