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Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly due to the…

Measuring the three dimension nanoscale organization of protein or cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied…

Powerful mid-infrared illumination combined with mechanical detection via force microscopy provides access to nanoscale spectroscopic imaging in Materials and Life Sciences. Photo-induced force microscopy (PiFM) employs pulsed illumination…

Optics · Physics 2025-09-09 Shohely Tasnim Anindo , Daniela Täuber , Christin David

Three-dimensional imaging plays an important role in imaging applications where it is necessary to record depth. The number of applications that use depth imaging is increasing rapidly, and examples include self-driving autonomous vehicles…

Image and Video Processing · Electrical Eng. & Systems 2021-04-21 Alice Ruget , Stephen McLaughlin , Robert K. Henderson , Istvan Gyongy , Abderrahim Halimi , Jonathan Leach

From multi-photon imaging penetrating millimeters deep through scattering biological tissue, to super-resolution imaging conquering the diffraction limit, optical imaging techniques have greatly advanced in recent years. Notwithstanding, a…

Ultrafast optical imaging has enabled direct observation of femtosecond-nanosecond dynamics, yet three-dimensional (3D) dynamic measurements at high numerical aperture (NA) remain hindered by the intrinsically shallow depth of field (DoF)…

We investigated the dynamics of single soft nano-tubes of phospholipids to extract nano-scale information such as size of tube, which are several tens to hundreds of nano-meters thick. The scaling law of the nano-tube dynamics in…

Soft Condensed Matter · Physics 2012-12-19 Akihisa Yamamoto , Masatoshi Ichikawa

Photodynamically active fibres (PAFs) are a novel class of stimulus-sensitive systems capable of triggering antibiotic-free antibacterial effect on-demand when exposed to light. Despite their relevance in infection control, however, the…

Tissues and Organs · Quantitative Biology 2021-01-07 Amy Contreras , Michael J. Raxworthy , Simon Wood , Giuseppe Tronci

Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have…

Nonlinear optical (NLO) imaging has emerged as a promising plant cell imaging technique due to its large optical penetration, inherent 3D spatial resolution, and reduced photodamage, meanwhile exogenous nanoprobes are usually needed for…

Optics · Physics 2023-08-23 Kun Liu , Yutian Lei , Dawei Li

Polarization microscopy provides insights into the structure and orientational organization of biomolecules and their architectures in cells. The above key functional signatures, which are natively 3D, can be only detected in 2D for a…

Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit…

Plasmonic hyperbolic metasurfaces have emerged as an effective platform for manipulating the propagation of light. Here, confined modes on arrays of silver nanoridges that exhibit hyperbolic dispersion are used to demonstrate and model a…

Optical microscopy has a diffraction limited resolution of about 250 nm. Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are still limited to 10 s of nm, and the images are an indirect pointillist representation of only…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Fluorescent imaging in the second near infrared window (NIR II, 1-1.4 {\mu}m) holds much promise due to minimal autofluorescence and tissue scattering. Here, using well functionalized biocompatible single-walled carbon nanotubes (SWNTs) as…

Biological Physics · Physics 2011-05-19 Kevin Welsher , Sarah P. Sherlock , Hongjie Dai

Positron emission tomography (PET) image denoising, along with lesion and organ segmentation, are critical steps in PET-aided diagnosis. However, existing methods typically treat these tasks independently, overlooking inherent synergies…

Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens.…

Instrumentation and Detectors · Physics 2019-10-01 Zichao Bian , Shaowei Jiang , Pengming Song , He Zhang , Pouria Hoveida , Kazunori Hoshino , Guoan Zheng

The number of eigenmodes in plasmonic nanostructures increases with complexity due to mode hybridization, raising the need for efficient mode characterization and selection. Here we experimentally demonstrate direct imaging and selective…

Raman spectroscopy enables non-destructive, label-free imaging with unprecedented molecular contrast but is limited by slow data acquisition, largely preventing high-throughput imaging applications. Here, we present a comprehensive…

Image and Video Processing · Electrical Eng. & Systems 2021-12-02 Conor C. Horgan , Magnus Jensen , Anika Nagelkerke , Jean-Phillipe St-Pierre , Tom Vercauteren , Molly M. Stevens , Mads S. Bergholt