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Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be made in tools that rely on classical methods for segmentation,…

Quantitative Methods · Quantitative Biology 2024-03-15 Nodar Gogoberidze , Beth A. Cimini

Cell segmentation for multi-modal microscopy images remains a challenge due to the complex textures, patterns, and cell shapes in these images. To tackle the problem, we first develop an automatic cell classification pipeline to label the…

Computer Vision and Pattern Recognition · Computer Science 2023-10-24 Wei Lou , Xinyi Yu , Chenyu Liu , Xiang Wan , Guanbin Li , Siqi Liu , Haofeng Li

Accurate segmentation of skin lesions within dermoscopic images plays a crucial role in the timely identification of skin cancer for computer-aided diagnosis on mobile platforms. However, varying shapes of the lesions, lack of defined…

Computer Vision and Pattern Recognition · Computer Science 2024-09-04 Hamza Farooq , Zuhair Zafar , Ahsan Saadat , Tariq M Khan , Shahzaib Iqbal , Imran Razzak

Medical image segmentation is crucial for accurate clinical diagnoses, yet it faces challenges such as low contrast between lesions and normal tissues, unclear boundaries, and high variability across patients. Deep learning has improved…

Image and Video Processing · Electrical Eng. & Systems 2024-12-09 Houze Liu , Tong Zhou , Yanlin Xiang , Aoran Shen , Jiacheng Hu , Junliang Du

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

We report an in-silico demonstration of an all-optical cell classification system using a single-layer diffractive neural network (DNN) optimized for real-world biomedical images. Implemented virtually with a spatial light modulator (SLM),…

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

Accurate detection and segmentation of cone cells in the retina are essential for diagnosing and managing retinal diseases. In this study, we used advanced imaging techniques, including confocal and non-confocal split detector images from…

Image and Video Processing · Electrical Eng. & Systems 2024-10-22 Prajol Shrestha , Mikhail Kulyabin , Aline Sindel , Hilde R. Pedersen , Stuart Gilson , Rigmor Baraas , Andreas Maier

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Developing clinically useful cell-level analysis tools in digital pathology remains challenging due to limitations in dataset granularity, inconsistent annotations, high computational demands, and difficulties integrating new technologies…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Nikita Shvetsov , Thomas K. Kilvaer , Masoud Tafavvoghi , Anders Sildnes , Kajsa Møllersen , Lill-Tove Rasmussen Busund , Lars Ailo Bongo

Deep learning has proven to be more effective than other methods in medical image analysis, including the seemingly simple but challenging task of segmenting individual cells, an essential step for many biological studies. Comparative…

Image and Video Processing · Electrical Eng. & Systems 2023-11-07 Valentina Vadori , Antonella Peruffo , Jean-Marie Graïc , Livio Finos , Livio Corain , Enrico Grisan

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Accurate cell segmentation is critical for biological and medical imaging studies. Although recent deep learning models have advanced this task, most methods are limited to generic cell segmentation, lacking the ability to differentiate…

Computer Vision and Pattern Recognition · Computer Science 2026-03-17 Bisheng Wang , Jaime S. Cardoso , Lin Wu

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…

Computer Vision and Pattern Recognition · Computer Science 2019-01-08 Assaf Arbelle , Tammy Riklin Raviv

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

Computer Vision and Pattern Recognition · Computer Science 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

Semantic image segmentation is one of the most important tasks in medical image analysis. Most state-of-the-art deep learning methods require a large number of accurately annotated examples for model training. However, accurate annotation…

Computer Vision and Pattern Recognition · Computer Science 2020-04-21 Ning Zhang , Susan Francis , Rayaz Malik , Xin Chen
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