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The genomic DNA of bacteria occupies only a fraction of the cell called the nucleoid, although it is not bounded by any membrane and would occupy a volume hundreds of times larger than the cell in the absence of constraints. The two most…

Biological Physics · Physics 2023-11-13 Marc Joyeux

The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication. Here we review recent advances in understanding the properties of…

Biomolecules · Quantitative Biology 2007-05-23 Leonor Saiz , Jose M. G. Vilar

From a nanoscience perspective, cellular processes and their reduced in vitro imitations provide extraordinary examples for highly robust few or single molecule reaction pathways. A prime example are biochemical reactions involving DNA…

Biological Physics · Physics 2007-05-23 Ralf Metzler , Tobias Ambjoernsson , Andreas Hanke , Yongli Zhang , Stephen Levene

DNA deformations play crucial roles in many biological processes and material applications. During DNA deformation, DNA structural parameters often exhibit non-trivial and counterintuitive couplings, such as the twist-stretch and…

Biological Physics · Physics 2021-10-08 Chen Zhang , Fujia Tian , Ying Lu , Bing Yuan , Zhi-Jie Tan , Xing-Hua Zhang , Liang Dai

Unbinding of a double-stranded DNA reduces to an unscreened long range interaction and maps on various problems. Heterogeneity renormalizes interaction. Renormalization is temperature dependent. At an unbinding transition it approaches…

Statistical Mechanics · Physics 2007-05-23 Mark Ya. Azbel'

The sequence-dependent elasticity of double-helical DNA on a nm length scale can be captured by the rigid base-pair model, whose strains are the relative position and orientation of adjacent base-pairs. Corresponding elastic potentials have…

Biomolecules · Quantitative Biology 2013-05-29 Nils B. Becker , Ralf Everaers

We investigate the dynamics of DNA translocation through a nanopore driven by an external force using Langevin dynamics simulations in two dimensions (2D) to study how the translocation dynamics depend on the details of the DNA sequences.…

Soft Condensed Matter · Physics 2008-12-09 Kaifu Luo , Tapio Ala-Nissila , See-Chen Ying , Aniket Bhattacharya

We report model calculations on DNA single strands which describe the equilibrium dynamics and kinetics of hairpin formation and melting. Modeling is at the level of single bases. Strand rigidity is described in terms of simple polymer…

Soft Condensed Matter · Physics 2007-10-01 Jalal Errami , Michel Peyrard , Nikos Theodorakopoulos

DNA-coated colloids can self-assemble into an incredible diversity of crystal structures, but applications of this technology are limited by poor understanding and control over the dynamical crystallization pathways. To address this…

Soft Condensed Matter · Physics 2021-06-01 Alexander Hensley , William M. Jacobs , W. Benjamin Rogers

We study the effect of transcription on the kinetics of DNA supercoiling in 3D by means of Brownian dynamics simulations of a single nucleotide resolution coarse-grained model for double stranded DNA. By accounting for the action of a…

Biological Physics · Physics 2025-02-03 Y. A. G. Fosado , D. Michieletto , C. A. Brackley , D. Marenduzzo

A theory for thermomechanical behavior of homogeneous DNA at thermal equilibrium predicts critical temperatures for denaturation under torque and stretch, phase diagrams for stable B--DNA, supercoiling, optimally stable torque, and the…

Soft Condensed Matter · Physics 2015-05-27 Cristiano Nisoli , A. R. Bishop

We investigate the measurement of DNA supercoiling density ($\sigma$) along chromosomes using interaction frequencies between DNA and DNA-anchored clusters of proteins. Specifically, we show how the physics of DNA supercoiling leads, in…

We study the effect of crowders on the melting profile of homogeneous and heterogeneous DNA molecules. We find out the melting profile of short DNA molecules and compare our findings with the experiments. We consider some random…

Soft Condensed Matter · Physics 2022-02-10 Neha Mathur , Amar Singh , Navin Singh

For short DNA molecules in crowded environments, we evaluate macroscopic parameters such as the average end-to-end distance and the twist conformation by tuning the strength of the site specific confinement driven by the crowders. The…

Soft Condensed Matter · Physics 2019-06-14 Marco Zoli

By means of the Brownian dynamics (BD) method of simulations we have developed, we study dynamics of individual DNA undergoing constant field gel electrophoresis (CFGE), focusing on the relevance of the `defect' concept due to de Gennes in…

Soft Condensed Matter · Physics 2007-05-23 Ryuzo Azuma

We present a self-contained theory for the mechanical response of DNA in single molecule experiments. Our model is based on a 1D continuum description of the DNA molecule and accounts both for its elasticity and for DNA-DNA electrostatic…

Biomolecules · Quantitative Biology 2015-05-13 N. Clauvelin , B. Audoly , S. Neukirch

The interplay between global constraints and local material properties of chain molecules is a subject of emerging interest. Studies of molecules that are intrinsically chiral, such as double-stranded DNA, is one example. Their properties…

Biological Physics · Physics 2013-11-27 Jakob Bohr , Kasper W. Olsen

Fluorescence microscopy reveals that the contents of many (membrane-free) nuclear "bodies" exchange rapidly with the soluble pool whilst the underlying structure persists; such observations await a satisfactory biophysical explanation. To…

Biological Physics · Physics 2017-04-26 C. A. Brackley , B. Liebchen , D. Michieletto , F. Mouvet , P. R. Cook , D. Marenduzzo

We report studies of the equilibrium and the dynamics of a general set of lattice models which capture the essence of the force-induced or mechanical DNA unzipping transition. Besides yielding the whole equilibrium phase diagram in the…

Soft Condensed Matter · Physics 2009-11-07 D. Marenduzzo , S. M. Bhattacharjee , A. Maritan , E. Orlandini , F. Seno

The non-thermal nature of self-propelling colloids offers new insights into non-equilibrium physics. The central mathematical model to describe their trajectories is active Brownian motion, where a particle moves with a constant speed,…