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3D image segmentation plays an important role in biomedical image analysis. Many 2D and 3D deep learning models have achieved state-of-the-art segmentation performance on 3D biomedical image datasets. Yet, 2D and 3D models have their own…

Computer Vision and Pattern Recognition · Computer Science 2018-12-11 Hao Zheng , Yizhe Zhang , Lin Yang , Peixian Liang , Zhuo Zhao , Chaoli Wang , Danny Z. Chen

The mechanical properties of polymer gels based on cytoskeleton proteins (e.g. actin) have been studied extensively due to their significant role in biological cell motility and in maintaining the cell's structural integrity. Microrheology…

Soft Condensed Matter · Physics 2014-08-22 Adar Sonn-Segev , Anne Bernheim-Groswasser , Yael Roichman

Cryo-electron tomography enables 3D visualization of cells in a near native state at molecular resolution. The produced cellular tomograms contain detailed information about all macromolecular complexes, their structures, their abundances…

Quantitative Methods · Quantitative Biology 2019-04-30 Min Xu , Elitza I Tocheva , Yi-Wei Chang , Grant J Jensen , Frank Alber

Cellular Electron CryoTomography (CECT) is a 3D imaging technique that captures information about the structure and spatial organization of macromolecular complexes within single cells, in near-native state and at sub-molecular resolution.…

Quantitative Methods · Quantitative Biology 2018-07-04 Kai Wen Wang , Xiangrui Zeng , Xiaodan Liang , Zhiguang Huo , Eric P. Xing , Min Xu

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Identification and quantification of nuclei in colorectal cancer haematoxylin \& eosin (H\&E) stained histology images is crucial to prognosis and patient management. In computational pathology these tasks are referred to as nuclear…

Quantitative Methods · Quantitative Biology 2022-03-08 Muhammad Dawood , Raja Muhammad Saad Bashir , Srijay Deshpande , Manahil Raza , Adam Shephard

When delineating lesions from medical images, a human expert can always keep in mind the anatomical structure behind the voxels. However, although high-quality (though not perfect) anatomical information can be retrieved from computed…

Image and Video Processing · Electrical Eng. & Systems 2023-12-04 Rongzhao Zhang , Zhian Bai , Ruoying Yu , Wenrao Pang , Lingyun Wang , Lifeng Zhu , Xiaofan Zhang , Huan Zhang , Weiguo Hu

Cryo-electron tomography (cryo-ET) is an imaging technique that allows three-dimensional visualization of macro-molecular assemblies under near-native conditions. Cryo-ET comes with a number of challenges, mainly low signal-to-noise and…

Cryo-EM is a transformational paradigm in molecular biology where computational methods are used to infer 3D molecular structure at atomic resolution from extremely noisy 2D electron microscope images. At the forefront of research is how to…

Quantitative Methods · Quantitative Biology 2025-06-12 Shayan Shekarforoush , David B. Lindell , Marcus A. Brubaker , David J. Fleet

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Accurate image segmentation plays a crucial role in medical image analysis, yet it faces great challenges of various shapes, diverse sizes, and blurry boundaries. To address these difficulties, square kernel-based encoder-decoder…

Image and Video Processing · Electrical Eng. & Systems 2022-01-02 Qian Yu , Lei Qi , Luping Zhou , Lei Wang , Yilong Yin , Yinghuan Shi , Wuzhang Wang , Yang Gao

Single particle, cryogenic electron microscopy (cryo-EM) experiments now routinely produce high-resolution data for large proteins and their complexes. Building an atomic model into a cryo-EM density map is challenging, particularly when no…

Biomolecules · Quantitative Biology 2020-09-04 Po-Nan Li , Saulo H. P. de Oliveira , Soichi Wakatsuki , Henry van den Bedem

The actin cytoskeleton is a semiflexible biopolymer network whose morphology is controlled by a wide range of biochemical and physical factors. Actin is known to undergo a phase transition from a single-filament state to a bundled state by…

Nanoparticle superlattices consisting of ordered arrangements of nanoparticles exhibit unique optical, magnetic, and electronic properties arising from nanoparticle characteristics as well as their collective behaviors. Understanding how…

Materials Science · Physics 2025-01-09 Aanish Paruchuri , Carl Thrasher , A. J. Hart , Robert Macfarlane , Arthi Jayaraman

Advances in fluorescence microscopy enable acquisition of 3D image volumes with better image quality and deeper penetration into tissue. Segmentation is a required step to characterize and analyze biological structures in the images and…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Chichen Fu , Soonam Lee , David Joon Ho , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

In the field of transmission electron microscopy, data interpretation often lags behind acquisition methods, as image processing methods often have to be manually tailored to individual datasets. Machine learning offers a promising approach…

Image and Video Processing · Electrical Eng. & Systems 2021-07-07 C. K. Groschner , Christina Choi , M. C. Scott

Cryo-Electron Microscopy (Cryo-EM) is a Nobel prize-winning technology for determining the 3D structure of particles at near-atomic resolution. A fundamental step in the recovering of the 3D single-particle structure is to align its 2D…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 Koby Bibas , Gili Weiss-Dicker , Dana Cohen , Noa Cahan , Hayit Greenspan

Brain fiber tracts are widely used in studying brain diseases, which may lead to a better understanding of how disease affects the brain. The segmentation of brain fiber tracts assumed enormous importance in disease analysis. In this paper,…

Neurons and Cognition · Quantitative Biology 2020-11-04 Chaoqing Xu , Guodao Sun , Ronghua Liang , Xiufang Xu

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Collagen fibrils, cable-like assemblies of long biological molecules, are dominant components of connective tissues. Their determinant morphological and functional roles motivated a large number of studies concerning their formation and…

Soft Condensed Matter · Physics 2011-04-04 Jean Charvolin , Jean-François Sadoc
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