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Electron ptychography describes a family of algorithms which are used to enable the reconstruction of complex specimen transmission functions of a sample in order to obtain both phase and amplitude information, as applied within the realms…

Optics · Physics 2025-03-17 L. Clark , P. D. Nellist

Ptychography is now integrated as a tool in mainstream microscopy allowing quantitative and high-resolution imaging capabilities over a wide field of view. However, its ultimate performance is inevitably limited by the available coherent…

Computational Physics · Physics 2024-06-12 Wenhui Xu , Shoucong Ning , Pengju Sheng , Huixiang Lin , Angus I Kirkland , Yong Peng , Fucai Zhang

Ptychography provides highly efficient imaging in scanning transmission electron microscopy (STEM), but questions have remained over its applicability to strongly scattering samples such as those most commonly seen in materialsscience.…

Applied Physics · Physics 2022-09-07 C. Gao , C. Hofer , D. Jannis , A. Béché , J. Verbeeck , T. J. Pennycook

Ptychography is an imaging technique that enables nanometer-scale reconstruction of complex transmittance images by scanning objects with overlapping illumination patterns. However, the illumination function is typically unknown, which…

Image and Video Processing · Electrical Eng. & Systems 2025-01-14 Qiuchen Zhai , Gregery T. Buzzard , Kevin Mertes , Brendt Wohlberg , Charles A. Bouman

Tilting planar samples for multi-zone-axes observation is a routine procedure in electron microscopy. However, this process invariably introduces optical path differences in the electron beam across different sample positions, significantly…

Materials Science · Physics 2024-08-22 Jizhe Cui , Yi Zheng , Kang Sun , Wenfeng Yang , Haozhi Sha , Rong Yu

Ptychography provides high dose efficiency images that can reveal light elements next to heavy atoms. However, despite ptychography having an otherwise single signed contrast transfer function, contrast reversals can occur when the…

Applied Physics · Physics 2023-06-16 C. Gao , C. Hofer , T. J. Pennycook

A detailed analysis of ptychography for 3D phase reconstructions of thick specimens is performed. We introduce multi-focus ptychography, which incorporates a 4D-STEM defocus series to enhance the quality of 3D reconstructions along the beam…

Resolving single atoms in large-scale volumes has been a goal for atomic resolution microscopy for a long time. Electron microscopy has come close to this goal using a combination of advanced electron optics and computational imaging…

Materials Science · Physics 2023-11-07 Andrey Romanov , Min Gee Cho , Mary Cooper Scott , Colin Ophus , Philipp Pelz

Ptychography is an attractive advance of coherent diffraction imaging (CDI), which can provide high lateral resolution and wide field of view. The theoretical resolution of ptychography is dose-limited, therefore making ptychography…

Biological Physics · Physics 2025-01-09 Huixiang Lin , Fucai Zhang

There is a clear need for developments in characterisation techniques that provide detailed information about structure-function relationships in biology. Using electron microscopy to achieve high resolution while maintaining a broad field…

Quantitative Methods · Quantitative Biology 2023-09-12 Chen Huang , Judy S. Kim , Angus I. Kirkland

In photon upconverting core-shell nanoparticles, structure strongly dictates performance. Conventional imaging in scanning transmission electron microscopy has sufficient resolution to probe the atomic structure of these nanoparticles, but…

Ptychography is a computational imaging technique using multiple, overlapping, coherently illuminated snapshots to achieve nanometer resolution by solving a nonlinear phase-field recovery problem. Ptychography is vital for imaging of…

Image and Video Processing · Electrical Eng. & Systems 2021-12-10 Qiuchen Zhai , Brendt Wohlberg , Gregery T. Buzzard , Charles A. Bouman

Mapping conformational heterogeneity of macromolecules presents a formidable challenge to X-ray crystallography and cryo-electron microscopy, which often presume its absence. This has severely limited our knowledge of the conformations…

Biological Physics · Physics 2015-05-14 P. Schwander , R. Fung , G. N. Phillips , A. Ourmazd

Here, we evaluate multislice electron ptychography as a tool to carry out depth-resolved atomic resolution characterization of point defects, using silicon carbide as a case study. Through multislice electron scattering simulations and…

Materials Science · Physics 2025-05-15 Aaditya Bhat , Colin Gilgenbach , Junghwa Kim , Michael Xu , Menglin Zhu , James M. LeBeau

Atomic-resolution imaging with scanning transmission electron microscopy is a powerful tool for characterizing the nanoscale structure of materials, in particular features such as defects, local strains, and symmetry-breaking distortions.…

Ptychography is a promising phase retrieval technique for visible light, X-ray and electron beams. Conventional ptychography reconstructs the amplitude and phase of an object light from a set of the diffraction intensity patterns obtained…

Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report…

Electron ptychography provides highly sensitive, dose efficient phase images which can be corrected for aberrations after the data has been acquired. This is crucial when very precise quantification is required, such as with sensitivity to…

Materials Science · Physics 2023-07-27 Christoph Hofer , Timothy J. Pennycook

Transmission electron microscopes use electrons with wavelengths of a few picometers, potentially capable of imaging individual atoms in solids at a resolution ultimately set by the intrinsic size of an atom. Unfortunately, due to…

We propose algorithms based on an optimisation method for inverse multislice ptychography in, e.g. electron microscopy. The multislice method is widely used to model the interaction between relativistic electrons and thick specimens. Since…

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