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Quantitative phase imaging (QPI) enables visualization and quantitative extraction of the optical phase information of transparent samples. However, conventional QPI techniques typically rely on multi-frame acquisition or complex…

As a label-free imaging technique, quantitative phase imaging (QPI) provides optical path length information of transparent specimens for various applications in biology, materials science, and engineering. Multispectral QPI measures…

Optics · Physics 2023-08-29 Che-Yung Shen , Jingxi Li , Deniz Mengu , Aydogan Ozcan

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…

In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field,…

Optics · Physics 2014-05-02 Guoan Zheng , Roarke Horstmeyer , Changhuei Yang

Full-color imaging is significant in digital pathology. Compared with a grayscale image or a pseudo-color image that only contains the contrast information, it can identify and detect the target object better with color texture information.…

Image and Video Processing · Electrical Eng. & Systems 2022-04-26 Yuting Gao , Jiurun Chen , Aiye Wang , An Pan , Caiwen Ma , Baoli Yao

In this paper we present a method to robustly evaluate the quantitative accuracy of various tomographic phase microscopy (TPM) methods with a multiple scattering 3D-printed microphantom with known geometry and refractive index distribution.…

Fourier ptychographic microscopy (FPM) is a promising computational imaging technique with high resolution, wide field-of-view (FOV) and quantitative phase recovery. So far, a series of system errors that may corrupt the image quality of…

Image and Video Processing · Electrical Eng. & Systems 2023-03-29 An Pan , Aiye Wang , Junfu Zheng , Yuting Gao , Caiwen Ma , Baoli Yao

Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

Optics · Physics 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Fourier ptychographic microscopy (FPM) is a recently proposed computational imaging technique with both high resolution and wide field-of-view. In current FP experimental setup, the dark-field images with high-angle illuminations are easily…

Computer Vision and Pattern Recognition · Computer Science 2017-12-19 Yan Zhang , An Pan , Ming Lei , Baoli Yao

Differential Dynamic Microscopy (DDM) analyzes traditional real-space microscope images to extract information on sample dynamics in a way akin to light scattering, by decomposing each image in a sequence into Fourier modes, and evaluating…

Soft Condensed Matter · Physics 2017-11-10 Fabio Giavazzi , Paolo Edera , Peter J. Lu , Roberto Cerbino

Quantitative phase microscopies (QPMs) have been mainly used for applications in cell biology, for around 2 decades. In this article, we show how cross-grating phase microscopy (CGM), a high-resolution, high-sensitivity QPM, recently…

Optics · Physics 2022-02-09 Guillaume Baffou

Quantitative phase imaging (QPI) is a label-free technique that provides optical path length information for transparent specimens, finding utility in biology, materials science, and engineering. Here, we present quantitative phase imaging…

A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation (TIE) of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane…

Utilizing the Pauli equation based multislice method, introduced in Phys. Rev. Lett. 116, 127203 (2016), we study the atomic resolution differential phase contrast (DPC) imaging on an example of a hard magnet FePt with in-plane…

Materials Science · Physics 2019-06-05 Alexander Edström , Axel Lubk , Ján Rusz

Quasi-phase matching (QPM) is a technique extensively utilized in nonlinear optics for enhancing the efficiency and stability of frequency conversion processes. However, the conventional QPM relies on periodically poled ferroelectric…

We present cytometric classification of live healthy and cancer cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor…

Quantitative Methods · Quantitative Biology 2020-01-23 Darina Roitshtain , Lauren Wolbromsky , Evgeny Bal , Hayit Greenspan , Lisa L. Satterwhite , Natan T. Shaked

With the help of quantum entanglement, quantum dense metrology (QDM) is a technique that can perform the joint estimates of two conjugate quantities such as phase and amplitude modulations of an optical field with an accuracy beating the…

Quantum Physics · Physics 2022-02-02 Wei Du , J. F. Chen , Z. Y. Ou , Weiping Zhang

We compare the phase space slicing and dipole subtraction methods in the computation of the inclusive and differential next-to-leading order cross sections for heavy quark production in the simple process gamma^* -> Q Qbar. For the phase…

High Energy Physics - Phenomenology · Physics 2008-11-26 Tim Oliver Eynck , Eric Laenen , Lukas Phaf , Stefan Weinzierl

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek