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Transcription factor concentrations provide signals to cells that allow them to regulate gene expression to make correct cell fate decisions. Calculations for noise bounds in gene regulation suggest that clustering or cooperative binding of…

Biological Physics · Physics 2026-01-09 Tamara Mijatović , Aimée R. Kok , Merlijn Brüggen , Jos W. Zwanikken , Marianne Bauer

Transcription is regulated through binding factors to gene promoters to activate or repress expression, however, the mechanisms by which factors find targets remain unclear. Using single-molecule fluorescence microscopy, we determined in…

Biomolecules · Quantitative Biology 2017-04-11 Adam J. M. Wollman , Sviatlana Shashkova , Erik G. Hedlund , Rosmarie Friemann , Stefan Hohmann , Mark C. Leake

Transcription factors (TFs) are key regulators of gene expression. Based on the classical scenario in which the TF search process switches between one-dimensional motion along the DNA molecule and free Brownian motion in the nucleus, we…

Genomics · Quantitative Biology 2010-10-15 Godefroy Malherbe , David Holcman

Transcription is a fundamental cellular process, and the first step of gene expression. In human cells, it depends on the binding to chromatin of various proteins, including RNA polymerases and numerous transcription factors (TFs).…

In the simplest view of transcriptional regulation, the expression of a gene is turned on or off by changes in the concentration of a transcription factor (TF). We use recent data on noise levels in gene expression to show that it should be…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Curtis G Callan , William Bialek

Genes are often regulated in living cells by proteins called transcription factors (TFs) that bind directly to short segments of DNA in close proximity to specific genes. These binding sites have a conserved nucleotide appearance, which is…

Statistics Theory · Mathematics 2007-06-13 Shane T. Jensen , Jun S. Liu

Understanding how transcription factors (TFs) regulate mammalian gene expression in space and time is a central topic in biology. To activate a gene, a TF has first to diffuse in the available space of the nucleus until it reaches a target…

Subcellular Processes · Quantitative Biology 2018-11-19 Maxime Woringer , Xavier Darzacq

To regulate a particular gene, a transcription factor (TF) needs to bind a specific genome location. How is this genome address specified amid the presence of ~10^6-10^9 decoy sites? Our analysis of 319 known TF binding motifs clearly…

Genomics · Quantitative Biology 2009-01-21 Leonid A. Mirny , Zeba Wunderlich

Focusing on the DNA-bridging nucleoid proteins Fis and H-NS, and integrating several independent experimental and bioinformatic data sources, we investigate the links between chromosomal spatial organization and global transcriptional…

Genomics · Quantitative Biology 2010-12-17 Vittore F. Scolari , Bruno Bassetti , Bianca Sclavi , Marco Cosentino Lagomarsino

Distributional text clustering delivers semantically informative representations and captures the relevance between each word and semantic clustering centroids. We extend the neural text clustering approach to text classification tasks by…

Computation and Language · Computer Science 2020-11-25 Yekun Chai , Haidong Zhang , Shuo Jin

A crucial step in the regulation of gene expression is binding of transcription factor (TF) proteins to regulatory sites along the DNA. But transcription factors act at nanomolar concentrations, and noise due to random arrival of these…

Molecular Networks · Quantitative Biology 2016-02-10 Thomas R. Sokolowski , Aleksandra M. Walczak , William Bialek , Gašper Tkačik

Transcription factor (TF) molecules translocate by facilitated diffusion (a combination of 3D diffusion around and 1D random walk on the DNA). Despite the attention this mechanism received in the last 40 years, only a few studies…

Subcellular Processes · Quantitative Biology 2013-09-20 Nicolae Radu Zabet , Boris Adryan

Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

We present a theoretical model of facilitated diffusion of proteins in the cell nucleus. This model, which takes into account the successive binding/unbinding events of proteins to DNA, relies on a fractal description of the chromatin which…

Statistical Mechanics · Physics 2015-05-19 O. Benichou , C. Chevalier , B. Meyer , R. Voituriez

Co-localization of networks of genes in the nucleus is thought to play an important role in determining gene expression patterns. Based upon experimental data, we built a dynamical model to test whether pure diffusion could account for the…

Molecular Networks · Quantitative Biology 2012-03-06 Jing Kang , Bing Xu , Ye Yao , Wei Lin , Conor Hennessy , Peter Fraser , Jianfeng Feng

It often is emphasized that gene expression is noisy. A seemingly contradictory view is that control mechanisms have been optimized to squeeze as much information as possible out of a limited number of molecules. Here we revisit these…

Biological Physics · Physics 2025-12-17 Nicholas Lawson , William Bialek

Transcription Factors (TFs) are proteins that regulate gene expression. The regulation mechanism is via the binding of a TF to a specific part of the gene associated with it, the TF's target. The target of a specific TF corresponds to a…

Statistical Mechanics · Physics 2022-09-02 Ori Hachmo , Ariel Amir

Transcription factors (TFs) regulate gene expression through complex and co-operative mechanisms. While many TFs act together, the logic underlying TFs binding and their interactions is not fully understood yet. Most current approaches for…

Machine Learning · Computer Science 2026-03-13 Pietro Demurtas , Ferdinando Zanchetta , Giovanni Perini , Rita Fioresi

A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other, and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we…

Molecular Networks · Quantitative Biology 2013-03-07 Sahand Hormoz

Many cellular responses to surrounding cues require temporally concerted transcriptional regulation of multiple genes. In prokaryotic cells, a single-input-module motif with one transcription factor regulating multiple target genes can…

Subcellular Processes · Quantitative Biology 2019-06-19 Jingyu Zhang , Hengyu Chen , Ruoyan Li , David A. Taft , Guang Yao , Fan Bai , Jianhua Xing
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