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The tumor microenvironment (TME) is a spatially heterogeneous ecosystem where cellular interactions shape tumor progression and response to therapy. Multiplexed imaging technologies enable high-resolution spatial characterization of the…
Electromagnetic frequencies up to a few terahertz (THz) can yield real-time and noninvasive measurements on biological matter. Unfortunately, strong absorption in aqueous solutions and low spatial resolution return difficult free-space…
Over the preceding years, nickel (Ni) and its compounds have been increasingly employed in various aspects of human social life, metallurgical/industrial manufactures, healthcare, and chemical processes. Although Ni is considered an…
Mechanical force is an essential feature for many physical and biological processes.1-12 Remote measurement of mechanical signals with high sensitivity and spatial resolution is needed for diverse applications, including robotics,13…
Radiomics is a nascent field in quantitative imaging that uses advanced algorithms and considerable computing power to describe tumor phenotypes, monitor treatment response, and assess normal tissue toxicity quantifiably. Remarkable…
Microplastics are increasingly recognized as a global environmental health threat, yet their detection and characterization remain constrained by the cost, form factor, and throughput of existing analytical tools. Portable…
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…
While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements in fluorescence…
Fluorescent nanodiamonds (FNDs) have been exploited as sensitive quantum probes for nanoscale chemical and biological sensing applications, with the majority of demonstrations to date relying on the detection of single FNDs. This places…
Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…
Luminescent thermometers are highly effective in niche applications such as nanothermometry, in vivo imaging, and extreme conditions like high electromagnetic fields, radiation, and under mechanical or chemical stress. Advancing measurement…
Tumors are extremely heterogeneous and comprise of a number of intratumor microenvironments or sub-regions. These tumor microenvironments may interact with eac based on complex high-level relationships, which could provide important insight…
The tumor microenvironment (TME) plays a crucial role in cancer progression and treatment response, yet current methods for its comprehensive analysis in H&E-stained tissue slides face significant limitations in the diversity of tissue cell…
We report a theoretical study of clusters of evanescently-coupled 2D whispering-gallery (WG) mode optical micro-cavities (termed "photonic molecules") as chemosensing and biosensing platforms. Photonic molecules (PMs) supporting modes with…
Optical fiber-based platforms are increasingly explored as compact, minimally invasive tools for integrated photonic functionalities in biomedical applications. Among these, the combination of plasmonic heating and optical sensing on a…
The detection of atmospheric fluorescence light from extensive air showers has become a powerful tool for accurate measurements of the energy and mass of ultra-high energy cosmic ray particles. Employing large area imaging telescopes with…
This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…
Diamond containing the negatively charged nitrogen-vacancy (NV) center is emerging as a significant new system for magnetometry. However, most NV sensors require microscopes to collect the fluorescence signals and are therefore limited to…
Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…