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Related papers: Microsecond Time-Resolved Cryo-Electron Microscopy

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Motivation: Cellular Electron CryoTomography (CECT) enables 3D visualization of cellular organization at near-native state and in sub-molecular resolution, making it a powerful tool for analyzing structures of macromolecular complexes and…

Quantitative Methods · Quantitative Biology 2017-04-14 Min Xu , Xiaoqi Chai , Hariank Muthakana , Xiaodan Liang , Ge Yang , Tzviya Zeev-Ben-Mordehai , Eric Xing

We theoretically propose a multidimensional high-harmonic echo spectroscopy technique which utilizes strong optical fields to resolve coherent electron dynamics spanning an energy range of multiple electron Volts. Using our recently…

Microplastics are increasingly recognized as a global environmental health threat, yet their detection and characterization remain constrained by the cost, form factor, and throughput of existing analytical tools. Portable…

Applied Physics · Physics 2025-10-31 Sayedus Salehin , Syed Shaheer Uddin Ahmed , Uzay Tefek , Laura Weirauch , M. Selim Hanay

Compressed sensing has been discussed separately in spatial and temporal domains. Compressive holography has been introduced as a method that allows 3D tomographic reconstruction at different depths from a single 2D image. Coded exposure is…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Zihao Wang , Leonidas Spinoulas , Kuan He , Huaijin Chen , Lei Tian , Aggelos K. Katsaggelos , Oliver Cossairt

We elucidate the physics of the dynamical transition via 10-100ns molecular dynamics simulations at temperatures spanning 160-300K. By tracking the energy fluctuations, we show that the protein dynamical transition is marked by a cross-over…

Quantitative Methods · Quantitative Biology 2009-06-17 Osman Burak Okan , Ali Rana Atilgan , Canan Atilgan

Transmission Electron Microscopy (TEM) is a powerful tool for imaging material structure and characterizing material chemistry. Recent advances in data collection technology for TEM have enabled high-volume and high-resolution data…

Computer Vision and Pattern Recognition · Computer Science 2021-08-24 Josh Kacher , Yao Xie , Sven P. Voigt , Shixiang Zhu , Henry Yuchi , Jordan Key , Surya R. Kalidindi

Photoelectron circular dichroism (PECD) is a fascinating phenomenon both from a fundamental science aspect but also due to its emerging role as a highly sensitive analytic tool for chiral recognition in the gas phase. PECD has been studied…

Chemical Physics · Physics 2021-05-21 Alexander Kastner , Tom Ring , Hendrike Braun , Arne Senftleben , Thomas Baumert

Modern microscopy techniques are developing towards high-resolution imaging, and tremendous progress has been made in past decades; however, the imaging of individual biological macromolecules at atomic resolution using short-wavelength…

Optics · Physics 2022-01-12 Tatiana Latychevskaia

Today's high-power laser systems are capable of reaching photon intensities up to $10^{22}$ W/cm^2, generating plasmas when interacting with material. The high intensity and ultrashort laser pulse duration (fs) make direct observation of…

Cryo-electron microscopy (cryo-EM) is a powerful technique for determining high-resolution 3D biomolecular structures from imaging data. Its unique ability to capture structural variability has spurred the development of heterogeneous…

Computer Vision and Pattern Recognition · Computer Science 2025-01-17 Minkyu Jeon , Rishwanth Raghu , Miro Astore , Geoffrey Woollard , Ryan Feathers , Alkin Kaz , Sonya M. Hanson , Pilar Cossio , Ellen D. Zhong

Cryo-electron microscopy (cryo-EM) is a widely used technique for recovering the 3-D structure of biological molecules from a large number of experimentally generated noisy 2-D tomographic projection images of the 3-D structure, taken from…

Numerical Analysis · Mathematics 2025-11-17 Jeremy Hoskins , Yuehaw Khoo , Oscar Mickelin , Amit Singer , Yuguan Wang

Cryo-electron microscopy (cryo-EM) is a powerful technique in structural biology and drug discovery, enabling the study of biomolecules at high resolution. Significant advancements by structural biologists using cryo-EM have led to the…

Biomolecules · Quantitative Biology 2024-12-05 Yi Zhou , Yilai Li , Jing Yuan , Quanquan Gu

We combine stroboscopic laser excitation with stochastic photoactivation and super-resolution fluorescence imaging. This makes it possible to record hundreds of diffusion trajectories of small protein molecules in single bacterial cells…

Quantitative Methods · Quantitative Biology 2010-03-11 Brian P. English , Arash Sanamrad , Stoyan Tankov , Vasili Hauryliuk , Johan Elf

Traditional image acquisition for cryo focused ion-beam scanning electron microscopy tomography often sees thousands of images being captured over a period of many hours, with immense data sets being produced. When imaging beam sensitive…

*To be published in Springer Handbook of Surface Science (Springer Verlag) [Preprint]* The capability to display images containing chemical, magnetic and structural information and to perform spectroscopy and diffraction from a {\mu}m-sized…

Mesoscale and Nanoscale Physics · Physics 2018-12-06 Alessandro Sala

Imaging of liquids and cryogenic biological materials by electron microscopy has been recently enabled by innovative approaches for specimen preparation and the fast development of optimised instruments for cryo-enabled electron microscopy…

Knowledge of the molecular structure is key to understanding the function of participating molecules in photo-induced chemical reactions. Visualizing the nuclear dynamics of a photochemical reaction requires an ultrafast measurement…

Chemical Physics · Physics 2021-12-21 Kasra Amini

Scanning electron microscopy (SEM), a century-old technique, is today a ubiquitous method of imaging the surface of nanostructures. However, most SEM detectors simply count the number of secondary electrons from a material of interest, and…

Understanding protein conformational dynamics is essential for elucidating biological function but remains challenging due to the wide range of timescales and the complexity of collective motions. Enhanced sampling methods overcome…

Statistical Mechanics · Physics 2026-05-11 Souvik Mondal , Michael A. Sauer , Matthias Heyden

Interacting-Particle Reaction Dynamics (iPRD) simulates the spatiotemporal evolution of particles that experience interaction forces and can react with one another. The combination of interaction forces and reactions enable a wide range of…

Chemical Physics · Physics 2018-07-20 Christoph Fröhner , Frank Noé