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We present two methods to reveal protein-ligand unbinding mechanisms in biased unbinding simulations by clustering trajectories into ensembles representing unbinding paths. The first approach is based on a contact principal component…

Biological Physics · Physics 2022-10-03 Simon Bray , Victor Tänzel , Steffen Wolf

Studying the pathways of ligand-receptor binding is essential to understand the mechanism of target recognition by small molecules. The binding free energy and kinetics of protein-ligand complexes can be computed using molecular dynamics…

Chemical Physics · Physics 2023-08-14 Dhiman Ray , Michele Parrinello

Protein-ligand (un)binding simulations are a recent focus of biased molecular dynamics simulations. Such binding and unbinding can occur via different pathways in and out of a binding site. We here present a theoretical framework how to…

Soft Condensed Matter · Physics 2023-03-27 Steffen Wolf , Matthias Post , Gerhard Stock

Receptor-ligand interactions are essential for biological function and their binding strength is commonly explained in terms of static lock-and-key models based on molecular complementarity. However, detailed information of the full…

Biological Physics · Physics 2021-02-19 Felix Rico , Andreas Russek , Laura Gonzalez , Helmut Grubmuller , Simon Scheuring

Searching for reaction pathways describing rare events in large systems presents a long-standing challenge in chemistry and physics. Incorrectly computed reaction pathways result in the degeneracy of microscopic configurations and inability…

Chemical Physics · Physics 2019-06-18 J. Rydzewski , O. Valsson

The unbinding process of a protein-ligand complex of major biological interest was investigated by means of a computational approach at atomistic classical mechanical level. An energy minimisation-based technique was used to determine the…

Biological Physics · Physics 2009-11-13 Elsa S. Henriques , Andrey V. Solov'yov

Ligand diffusion through proteins is a fundamental process governing biological signaling and enzymatic catalysis. The complex topology of protein tunnels results in difficulties with computing ligand escape pathways by standard molecular…

Chemical Physics · Physics 2019-03-21 Jakub Rydzewski , Wieslaw Nowak

Determining the complete set of ligands' binding/unbinding pathways is important for drug discovery and to rationally interpret mutation data. Here we have developed a metadynamics-based technique that addressed this issue and allows…

Chemical Physics · Physics 2019-04-25 Riccardo Capelli , Paolo Carloni , Michele Parrinello

The capacity to identify and analyze protein-protein interactions, along with their internal modular organization, plays a crucial role in comprehending the intricate mechanisms underlying biological processes at the molecular level. We can…

Molecular Networks · Quantitative Biology 2023-08-11 Mamata Das , Selvakumar K. , P. J. A. Alphonse

A new self-learning algorithm for accelerated dynamics, reconnaissance metadynamics, is proposed that is able to work with a very large number of collective coordinates. Acceleration of the dynamics is achieved by constructing a bias…

Computational Physics · Physics 2010-10-15 Gareth A. Tribello , Michele Ceriotti , Michele Parrinello

To interpret molecular dynamics simulations of biomolecular systems, systematic dimensionality reduction methods are commonly employed. Among others, this includes principal component analysis (PCA) and time-lagged independent component…

Biomolecules · Quantitative Biology 2022-07-20 Georg Diez , Daniel Nagel , Gerhard Stock

Signaling proteins are an important topic in drug development due to the increased importance of finding fast, accurate and cheap methods to evaluate new molecular targets involved in specific diseases. The complexity of the protein…

Quantitative Methods · Quantitative Biology 2019-04-11 Carlos Fernandez-Lozano , Ruben F. Cuinas , Jose A. Seoane , Enrique Fernandez-Blanco , Julian Dorado , Cristian R. Munteanu

Accurate protein structural ensembles can be determined with metainference, a Bayesian inference method that integrates experimental information with prior knowledge of the system and deals with all sources of uncertainty and errors as well…

Quantitative Methods · Quantitative Biology 2019-01-24 Thomas Löhr , Carlo Camilloni , Massimiliano Bonomi , Michele Vendruscolo

We here report on non-equilibrium targeted Molecular Dynamics simulations as tool for the estimation of protein-ligand unbinding kinetics. Correlating simulations with experimental data from SPR kinetics measurements and X-ray…

Disentangling the mechanistic details of a chemical reaction pathway is a hard problem that often requires a considerable amount of chemical intuition and a component of luck. Experiments struggle in observing short-life metastable…

Chemical Physics · Physics 2019-08-01 Valerio Rizzi , Dan Mendels , Emilia Sicilia , Michele Parrinello

Protein-ligand interactions are crucial for a wide range of physiological processes. Many cellular functions result in these non-covalent `bonds' being mechanically strained, and this can be integral to proper cellular function. Broadly,…

Soft Condensed Matter · Physics 2022-03-31 Willmor J. Peña Ccoa , Glen M. Hocky

Protein-ligand complex structures have been utilised to design benchmark machine learning methods that perform important tasks related to drug design such as receptor binding site detection, small molecule docking and binding affinity…

Biomolecules · Quantitative Biology 2021-08-26 Rishal Aggarwal , Akash Gupta , U Deva Priyakumar

A major challenge in nonadiabatic molecular dynamics is to automatically and objectively identify the key reaction coordinates that drive molecules toward distinct excited-state decay channels. Traditional manual analyses are inefficient…

Chemical Physics · Physics 2025-11-18 Hangxu Liu , Yifei Zhu , Zhenggang Lan

Coarse-graining of fully atomistic molecular dynamics simulations is a long-standing goal in order to allow the description of processes occurring on biologically relevant timescales. For example, the prediction of pathways, rates and…

Biological Physics · Physics 2020-06-12 Steffen Wolf , Benjamin Lickert , Simon Bray , Gerhard Stock

Is it possible to understand the intricacies of a dynamical system not solely from its input/output pattern, but also by observing the behavior of other systems within the same class? This central question drives the study presented in this…

Systems and Control · Electrical Eng. & Systems 2023-12-21 Marco Forgione , Filippo Pura , Dario Piga
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