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Two-photon microscopy is a powerful tool for in vivo imaging, but its imaging depth is typically limited to a few hundred microns due to tissue scattering, even with existing scattering correction techniques. Moreover, most active…

Optics · Physics 2025-05-29 Renzhi He , Yucheng Li , Brianna Urbina , Jiandi Wan , Yi Xue

Two-photon (2P) microscopy is a powerful technique for deep-tissue fluorescence imaging; however, tissue scattering limits its effectiveness for depth imaging using conventional approaches. Despite typical strategies having been put forward…

Optics · Physics 2025-05-21 Lei Zhu , Bernhard Rauer , Hilton B. De Aguiar , Sylvain Gigan

Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here,…

Light scattering in inhomogeneous media induces wavefront distortions which pose an inherent limitation in many optical applications. Examples range from microscopy and nanosurgery to astronomy. In recent years, ongoing efforts have made…

Optics · Physics 2015-05-20 Ori Katz , Yaron Bromberg , Eran Small , Yaron Silberberg

Multiple scattering of waves in disordered media is a nightmare whether it be for detection or imaging purposes. The best approach so far to get rid of multiple scattering is optical coherence tomography. It basically combines confocal…

Optical focusing through/inside scattering media, like multimode fiber and biological tissues, has significant impact in biomedicine yet considered challenging due to strong scattering nature of light. Previously, promising progress has…

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

IR microspectroscopy of single biological cells is challenged by strong light scattering, which produces baseline effects and peak distortions in the IR spectra and hinders the direct extraction of chemical information. Current methods for…

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Wavefront-shaping is a promising approach for imaging fluorescent targets deep inside scattering tissue despite strong aberrations. It enables focusing an incoming illumination into a single spot inside tissue, as well as correcting the…

Optics · Physics 2024-01-23 Dror Aizik , Anat Levin

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

Imaging through opaque, highly scattering walls is a long sought after capability with potential applications in a variety of fields. The use of wavefront shaping to compensate for scattering has brought a renewed interest as a potential…

Compared to single-source imaging systems, dual-source imaging systems equipped with two cross-distributed scanning beams significantly enhance temporal resolution and capture more comprehensive object scanning information. Nevertheless,…

Medical Physics · Physics 2025-03-19 Jianing Sun , Jigang Duan , Guangyin Li , Xu Jiang , Xing Zhao

From multi-photon imaging penetrating millimeters deep through scattering biological tissue, to super-resolution imaging conquering the diffraction limit, optical imaging techniques have greatly advanced in recent years. Notwithstanding, a…

Non-invasive optical focusing inside scattering media is still a big challenge because inhomogeneous media scatter both incoming photons for focusing and outgoing photons for observation. Various approaches, utilizing non-linear…

Optics · Physics 2021-09-17 Dayan Li , Sujit Kumar Sahoo , Huy Quoc Lam , Dong Wang , Cuong Dang

The recent advent of wave-shaping methods has demonstrated the focusing of light through and inside even the most strongly scattering materials. Typically in wavefront shaping, light is focused in an area with the size of one speckle spot.…

In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. Based on a dynamic correction of the incident…

Focusing light through highly scattering materials by modifying the phase profile of the illuminating beam has attracted a great deal of attention in the past decade paving the way towards novel applications. Here we discovered a tradeoff…

Optics · Physics 2018-09-25 Eitan Edrei , Giuliano Scarcelli

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the…

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