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Related papers: Work Distribution for Unzipping Processes

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The two strands of the DNA double helix can be `unzipped' by application of 15 pN force. We analyze the dynamics of unzipping and rezipping, for the case where the molecule ends are separated and re-approached at constant velocity. For…

Soft Condensed Matter · Physics 2009-11-07 S. Cocco , R. Monasson , J. F. Marko

We study by using Monte Carlo simulations the hysteresis in unzipping and rezipping of a double stranded DNA (dsDNA) by pulling its strands in opposite directions in the fixed force ensemble. The force is increased, at a constant rate from…

Soft Condensed Matter · Physics 2012-10-11 Rajeev Kapri

We present an extensive theoretical investigation of the mechanical unzipping of double-stranded DNA under the influence of an applied force. In the limit of long polymers, there is a thermodynamic unzipping transition at a critical force…

Soft Condensed Matter · Physics 2009-11-07 David K. Lubensky , David R. Nelson

The mechanical separation of the double helical DNA structure induced by forces pulling apart the two DNA strands (``unzipping'') has been the subject of recent experiments. Analytical results are obtained within various models of…

Statistical Mechanics · Physics 2009-11-07 D. Marenduzzo , A. Trovato , A. Maritan

We investigate by Monte Carlo simulations the zipping and unzipping dynamics of two polymers connected by one end and subject to an attractive interaction between complementary monomers. In zipping, the polymers are quenched from a high…

Soft Condensed Matter · Physics 2011-02-15 Alessandro Ferrantini , Enrico Carlon

Mechanical unfolding trajectories, generated by applying constant force in optical tweezer experiments, show that RNA hairpins and the P5abc subdomain of the group I intron unfold reversibly. We use coarse-grained Go-like models for RNA…

Biomolecules · Quantitative Biology 2009-11-11 Changbong Hyeon , D. Thirumalai

We study a class of micromanipulation experiments, exemplified by the pulling apart of the two strands of double-stranded DNA (dsDNA). When the pulling force is increased to a critical value, an ``unzipping'' transition occurs. For random…

Soft Condensed Matter · Physics 2009-10-31 David K. Lubensky , David R. Nelson

We show how single-molecule unzipping experiments can provide strong evidence that the zero-force melting transition of long molecules of natural dsDNA should be classified as a phase transition of the higher-order type (continuous). We…

Soft Condensed Matter · Physics 2007-05-23 C. Brian Roland , Kristi Adamson Hatch , Mara Prentiss , Eugene I. Shakhnovich

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and…

Using coarse-grained model we have explored forced-unfolding of RNA hairpin as a function of $f_S$ and the loading rate ($r_f$). The simulations and theoretical analysis have been done without and with the handles that are explicitly…

Biomolecules · Quantitative Biology 2009-11-13 Changbong Hyeon , D. Thirumalai

We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder…

Soft Condensed Matter · Physics 2009-04-13 Buddhapriya Chakrabarti , David R. Nelson

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The…

Soft Condensed Matter · Physics 2015-06-18 Poulomi Sadhukhan , Somendra M. Bhattacharjee

We consider two complementary polymer strands of length $L$ attached by a common end monomer. The two strands bind through complementary monomers and at low temperatures form a double stranded conformation (zipping), while at high…

Statistical Mechanics · Physics 2015-03-19 Jean-Charles Walter , Alessandro Ferrantini , Enrico Carlon , Carlo Vanderzande

We derive and solve a differential equation satisfied by the probability distribution of the work done on a single biomolecule in a mechanical unzipping experiment. The unzipping is described as a thermally activated escape process in an…

Soft Condensed Matter · Physics 2007-05-23 Alberto Imparato , Luca Peliti

We discuss the thermodynamic behaviour near the force induced unzipping transition of a double stranded DNA in two different ensembles. The Y-fork is identified as the coexisting phases in the fixed distance ensemble. From finite size…

Soft Condensed Matter · Physics 2007-05-23 Rajeev Kapri , Somendra M. Bhattacharjee

We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two state manner under the action of mechanical force. We apply path thermodynamics to derive analytical expressions for the average…

Statistical Mechanics · Physics 2009-02-25 Maria Manosas , Alessandro Mossa , Nuria Forns , Josep Maria Huguet , Felix Ritort

Using the criterion that the mechanical unzipping transition in a bound homopolymer is triggered when the average force exerted by the single unbound strands on the first base pair in the bound section exceeds the force binding the pair…

Soft Condensed Matter · Physics 2007-05-23 Richard M. Neumann

Using theory and simulations, we carried out a first systematic characterization of DNA unzipping via nanopore translocation. Starting from partially unzipped states, we found three dynamical regimes depending on the applied force, f: (i)…

Soft Condensed Matter · Physics 2023-02-08 Antonio Suma , Vincenzo Carnevale , Cristian Micheletti

We review the basic concepts and tools for mechanically unzipping RNA hairpins using force spectroscopy. By pulling apart the ends of an RNA molecule using optical tweezers, it is possible to measure the folding free energy at varying…

Soft Condensed Matter · Physics 2023-03-27 Paolo Rissone , Isabel Pastor , Felix Ritort

We report studies of the equilibrium and the dynamics of a general set of lattice models which capture the essence of the force-induced or mechanical DNA unzipping transition. Besides yielding the whole equilibrium phase diagram in the…

Soft Condensed Matter · Physics 2009-11-07 D. Marenduzzo , S. M. Bhattacharjee , A. Maritan , E. Orlandini , F. Seno
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