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Polarization dynamics in ferroelectric materials are explored via the automated experiment in Piezoresponse Force Spectroscopy. A Bayesian Optimization framework for imaging is developed and its performance for a variety of acquisition and…

Disordered Systems and Neural Networks · Physics 2021-06-24 Rama K. Vasudevan , Kyle Kelley , Jacob Hinkle , Hiroshi Funakubo , Stephen Jesse , Sergei V. Kalinin , Maxim Ziatdinov

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

We present high resolution two-photon excitation microscopy studies combining two-photon fluorescence (TPF) and second harmonic generation (SHG) in order to probe orientational distributions of molecular ensembles at room temperature. A…

Soft Condensed Matter · Physics 2007-05-23 Veronique Le Floc'h , Sophie Brasselet , Jean-Francois Roch , Joseph Zyss

Frontal polymerization (FP) is an approach for thermosetting plastics at lower energy cost than an autoclave. The potential to generate simultaneous propagation of multiple polymerization fronts has been discussed as an exciting…

We propose a novel approach to detect the binding between proteins making use of the anomalous diffraction of natively present heavy elements inside the molecule 3D structure. In particular, we suggest considering sulfur atoms contained in…

Absolute quantification of biological samples entails determining expression levels in precise numerical copies, offering enhanced accuracy and superior performance for rare templates. However, existing methodologies suffer from significant…

Quantitative Methods · Quantitative Biology 2023-09-06 Yuanyuan Wei , Sai Mu Dalike Abaxi , Nawaz Mehmood , Luoquan Li , Fuyang Qu , Guangyao Cheng , Dehua Hu , Yi-Ping Ho , Scott Wu Yuan , Ho-Pui Ho

The functional properties of ferroelectric materials are strongly influenced by ferroelectric polarization orientation; as such, access to consistent and precise characterization of polarization vectors is of substantial importance to…

High-throughput screening using cell images is an efficient method for screening new candidates for pharmaceutical drugs. To complete the screening process, it is essential to have an efficient process for analyzing cell images. This paper…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Mizuki Fukasawa , Tomokazu Fukuda , Takuya Akashi

Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…

In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and…

Optics · Physics 2007-05-23 Laurent Cognet , Françoise Coussen , Daniel Choquet , Brahim Lounis

Polarization of the mammalian embryo at the right developmental time is critical for its development to term and would be valuable in assessing the potential of human embryos. However, tracking polarization requires invasive fluorescence…

Quantitative Methods · Quantitative Biology 2021-11-10 Cheng Shen , Adiyant Lamba , Meng Zhu , Ray Zhang , Changhuei Yang , Magdalena Zernicka Goetz

We propose and demonstrate a novel technique that combines Raman scattering and optical cycling in molecules with diagonal Franck-Condon factors. This resonance Raman optical cycling manipulates molecules to behave like efficient…

Atomic Physics · Physics 2021-08-26 J. C. Shaw , J. C. Schnaubelt , D. J. McCarron

The development of novel transparent conducting materials (TCMs) is essential for enhancing the performance and reducing the cost of next-generation devices such as solar cells and displays. In this research, we focus on the…

Materials Science · Physics 2025-08-01 Daisuke Makino , Tatsuya Goto , Yoshinori Suga

Efficient and high-fidelity polarization demosaicking is critical for industrial applications of the division of focal plane (DoFP) polarization imaging systems. However, existing methods have an unsatisfactory balance of speed, accuracy,…

Image and Video Processing · Electrical Eng. & Systems 2024-09-02 Guangsen Liu , Peng Rao , Xin Chen , Yao Li , Haixin Jiang

The polarization of light conveys crucial information about the spatial ordering and optical properties of a specimen. However, precise polarization measurement in challenging conditions, including constrained spaces, low light levels, and…

Optics · Physics 2026-03-10 Martin Bielak , Dominik Vašinka , Miroslav Ježek

We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a…

Optics · Physics 2015-05-13 Kevin McHale , Hideo Mabuchi

Angular filter refractometry is an optical diagnostic that measures absolute contours of line-integrated density gradient by placing a filter with alternating opaque and transparent zones in the focal plane of a probe beam, which produce…

Plasma Physics · Physics 2023-11-30 P. V. Heuer , D. Haberberger , S. T. Ivancic , C. A. Walsh , J. R. Davies

Single molecule microscopy has proven to be an effective tool to characterize the fluorophore-plasmonic structure interaction. However, as specific information is hidden in the emission, sophisticated evaluation is required. Here we…

Optics · Physics 2024-01-11 T. Novák , P. Bíró , Gy. Ferenc , D. Ungor , E. Czvik , Á. Deák , L. Janovák , M. Erdélyi

As an essential attribute of organic compounds, polarity has a profound influence on many molecular properties such as solubility and phase transition temperature. Thin layer chromatography (TLC) represents a commonly used technique for…

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially…

Subcellular Processes · Quantitative Biology 2009-03-09 George D Tsibidis
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