Related papers: Live-cell imaging powered by computation
Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…
Exposure to intense illumination light is an unavoidable consequence of fluorescence microscopy, and poses a risk to the health of the sample in every live-cell fluorescence microscopy experiment. Furthermore, the possible side-effects of…
The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…
Imaging is an important means by which information is gathered regarding the physical world. Spatial resolution and signal-to-noise ratio are underpinning concepts. There is a paucity of rigorous definitions for these quantities, which are…
Magnetic resonance imaging (MRI) is the cornerstone technique for diagnostic medicine, biology, and neuroscience. This imaging method is highly innovative, noninvasive and its impact continues to grow. It can be used for measuring changes…
An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…
We propose a supervised machine learning approach for boosting existing signal and image recovery methods and demonstrate its efficacy on example of image reconstruction in computed tomography. Our technique is based on a local nonlinear…
The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…
Neuromorphic Computing implemented in photonic hardware is one of the most promising routes towards achieving machine learning processing at the picosecond scale, with minimum power consumption. In this work, we present a new concept for…
Traditional fluorescence microscopy is constrained by inherent trade-offs among resolution, field-of-view, and system complexity. To navigate these challenges, we introduce a simple and low-cost computational multi-aperture miniature…
Computer-aided design for synthetic biology promises to accelerate the rational and robust engineering of biological systems; it requires both detailed and quantitative mathematical and experimental models of the processes to (re)design,…
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…
Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…
Multicolor cell spatio-temporal image data have become important to investigate organ development and regeneration, malignant growth or immune responses by tracking different cell types both in vivo and in vitro. Statistical modeling of…
Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…
To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…
Lightsheet microscopy is a powerful 3-D imaging technique that addresses limitations of traditional optical and confocal microscopy but suffers from a low penetration depth and reduced image quality at greater depths. Multiview lightsheet…
Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…
Optical microscopy is an indispensable tool in life sciences research, but conventional techniques require compromises between imaging parameters like speed, resolution, field-of-view, and phototoxicity. To overcome these limitations,…
Understanding the behaviour of biological systems requires a complex setting of in vitro and in vivo experiments, which attracts high costs in terms of time and resources. The use of mathematical models allows researchers to perform…