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We demonstrate focusing and imaging through a scattering medium noninvasively by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning…

Optics · Physics 2019-07-25 Anat Daniel , Dan Oron , Yaron Silberberg

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

Optical imaging through scattering media is a long-standing challenge. Although many approaches have been developed to focus light or image objects through scattering media, they are either invasive, restricted to stationary or…

Optics · Physics 2022-10-11 Xingchen Zhao , Xiaoyu Nie , Zhenhuan Yi , Tao Peng , Marlan O. Scully

We propose and demonstrate a new imaging technique to noninvasively see through scattering layers with the aid of a spatial light modulator (SLM). A relay system projects the incoherent light pattern emitting from the scattering layer onto…

Optics · Physics 2019-05-30 Saswata Mukherjee , A. Vijayakumar , Joseph Rosen

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Imaging with optical resolution through highly scattering media is a long sought-after goal with important applications in deep tissue imaging. Although being the focus of numerous works, this goal was considered impractical until recently.…

Optics · Physics 2012-02-10 Ori Katz , Eran Small , Yaron Silberberg

There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…

Computer Vision and Pattern Recognition · Computer Science 2016-05-16 Henryk Blasinski , Joyce Farrell , Brian Wandell

We study active array imaging of small but strong scatterers in homogeneous media when multiple scattering between them is important. We use the Foldy-Lax equations to model wave propagation with multiple scattering when the scatterers are…

Mathematical Physics · Physics 2015-12-15 Anwei Chai , Miguel Moscoso , George Papanicolaou

Three-dimensional (3D) high-resolution imaging is essential in microscopy, yet light scattering poses significant challenges in achieving it. Here, we present an approach to holographic imaging of spatially incoherent objects through…

Optics · Physics 2025-02-04 YoonSeok Baek , Hilton B. de Aguiar , Sylvain Gigan

A robust method and strategy for efficient full field-ofview and depth separation optical imaging through scattering media regardless of the three-dimensional (3D) optical memory effect are proposed. In this method, the problem of imaging…

Optics · Physics 2020-01-17 Wei Li , Jietao Liu , Shunfu He , Lixian Liu , Xiaopeng Shao

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Multiple scattering of waves in disordered media is a nightmare whether it be for detection or imaging purposes. The best approach so far to get rid of multiple scattering is optical coherence tomography. It basically combines confocal…

We present a laser scanning reflection-matrix microscopy combining the scanning of laser focus and the wide-field mapping of the electric field of the backscattered waves for eliminating higher-order aberrations even in the presence of…

Biological Physics · Physics 2019-10-11 Seokchan Yoon , Hojun Lee , Jin Hee Hong , Yong-Sik Lim , Wonshik Choi

Imaging through complex scattering media is severely limited by aberrations and scattering which obscure images and reduce resolution. Confocal and temporal gatings partly filter out multiple scattering but are severely degraded by…

Optics · Physics 2026-05-18 Yiwen Zhang , Minh Dinh , Zeyu Wang , Tianhao Zhang , Tianhang Chen , Chia Wei Hsu

Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…

High-resolution optical microscopy has transformed biological imaging, yet its resolution and contrast deteriorate with depth due to multiple light scattering. Conventional correction strategies typically approximate the medium as one or a…

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig