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The mode of action of proteins is to a large extent given by their ability to adopt different conformations. This is why imaging single biomolecules at atomic resolution is one of the ultimate goals of biophysics and structural biology. The…

Biological Physics · Physics 2012-09-27 Jean-Nicolas Longchamp , Tatiana Latychevskaia , Conrad Escher , Hans-Werner Fink

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

Single molecule X-ray scattering experiments with free electron lasers have opened a new route to the structure determination of biomolecules. Because typically only very few photons per scattering image are recorded and thus the…

Computational Physics · Physics 2023-04-17 Steffen Schultze , Helmut Grubmüller

X-ray free-electron lasers (XFELs) may allow to employ the single particle imaging (SPI) method to determine the structure of macromolecules that do not form stable crystals. Ultrashort pulses of 10 fs and less allow to outrun complete…

Biological Physics · Physics 2015-11-20 O. Yu. Gorobtsov , U. Lorenz , N. M. Kabachnik , I. A. Vartanyants

This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…

Optics · Physics 2023-12-25 Tamme Wollweber , Kartik Ayyer

Imperfections in X-ray imaging systems can limit their performance, especially in High Energy Density (HED) or Inertial Fusion Energy (IFE)-relevant experiments that are typically single shot, by introducing structured, non-stationary…

The European X-ray Free Electron Laser (XFEL) will deliver 30,000 fully coherent, high brilliance X-ray pulses per second each with a duration below 100 fs. This will allow the recording of diffraction patterns of single complex molecules…

Instrumentation and Detectors · Physics 2015-06-03 Jiaguo Zhang , Eckhart Fretwurst , Robert Klanner , Hanno Perrey , Ioana Pintilie , Thomas Poehlsen , Joern Schwandt

Since Perutz, Kendrew and colleagues unveiled the structure of hemoglobin and myoglobin based on X-ray diffraction analysis in the 1950s, X-ray crystallography has become the primary methodology used to determine the 3D structure of…

High-energy X-ray diffraction methods can non-destructively map the 3D microstructure and associated attributes of metallic polycrystalline engineering materials in their bulk form. These methods are often combined with external stimuli…

Accurate characterization of radiation pulse profiles is crucial for optimizing beam quality and enhancing experimental outcomes in Free Electron Laser (FEL) research. In this paper, we present a novel approach that employs machine learning…

Accelerator Physics · Physics 2024-11-27 Till Korten , Vladimir Rybnikov , Peter Steinbach , Najmeh Mirian

Reconstructing unbounded outdoor scenes from sparse outward-facing views poses significant challenges due to minimal view overlap. Previous methods often lack cross-scene understanding and their primitive-centric formulations overload local…

Computer Vision and Pattern Recognition · Computer Science 2025-02-07 Eyvaz Najafli , Marius Kästingschäfer , Sebastian Bernhard , Thomas Brox , Andreas Geiger

X-ray Free Electron Lasers (X\nobreakdash-FELs) operate in a wide range of lasing configurations for a broad variety of scientific applications at ultrafast time-scales such as structural biology, materials science, and atomic and molecular…

Single-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification. However, the…

Biomolecules · Quantitative Biology 2024-11-26 Tong You , Johan Bielecki , Filipe R. N. C. Maia

X-ray ptychography allows for large fields to be imaged at high resolution at the cost of additional computational expense due to the large volume of data. Given limited information regarding the object, the acquired data often has an…

Image and Video Processing · Electrical Eng. & Systems 2022-07-01 Dergan Lin , Yi Jiang , Junjing Deng , Zichao Wendy Di

Single-pixel imaging (SPI) is significant for applications constrained by transmission bandwidth or lighting band, where 3D SPI can be further realized through capturing signals carrying depth. Sampling strategy and reconstruction algorithm…

Image and Video Processing · Electrical Eng. & Systems 2025-04-01 Xinyue Ma , Chenxing Wang

X-ray scattering experiments using Free Electron Lasers (XFELs) are a powerful tool to determine the molecular structure and function of unknown samples (such as COVID-19 viral proteins). XFEL experiments are a challenge to computing in two…

Distributed, Parallel, and Cluster Computing · Computer Science 2024-03-21 Johannes P. Blaschke , Aaron S. Brewster , Daniel W. Paley , Derek Mendez , Asmit Bhowmick , Nicholas K. Sauter , Wilko Kröger , Murali Shankar , Bjoern Enders , Deborah Bard

We present here an overview of Coherent X-ray Diffraction Imaging (CXDI) with its application to nanostructures. This imaging approach has become especially important recently due to advent of X-ray Free-Electron Lasers (XFEL) and its…

Mesoscale and Nanoscale Physics · Physics 2013-04-22 Ivan A. Vartanyants , Oleksandr M. Yefanov

X-ray imaging is indispensable in medical diagnostics, yet its use is tightly regulated due to potential health risks. To mitigate radiation exposure, recent research focuses on generating novel views from sparse inputs and reconstructing…

Computer Vision and Pattern Recognition · Computer Science 2025-03-12 Feiran Wang , Jiachen Tao , Junyi Wu , Haoxuan Wang , Bin Duan , Kai Wang , Zongxin Yang , Yan Yan

Single-pixel imaging (SPI) is a novel imaging technique whose working principle is based on the compressive sensing (CS) theory. In SPI, data is obtained through a series of compressive measurements and the corresponding image is…

Image and Video Processing · Electrical Eng. & Systems 2022-07-15 Stephen L. H. Lau , Edwin K. P. Chong

The X-ray microscopy technique at the European X-ray free-electron laser (EuXFEL), operating at a MHz repetition rate, provides superior contrast and spatial-temporal resolution compared to typical microscopy techniques at other X-ray…