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Cell clustering is crucial for uncovering cellular heterogeneity in single-cell RNA sequencing (scRNA-seq) data by identifying cell types and marker genes. Despite its importance, benchmarks for scRNA-seq clustering methods remain…

Genomics · Quantitative Biology 2025-12-03 Ping Xu , Zaitian Wang , Zhirui Wang , Pengjiang Li , Jiajia Wang , Ran Zhang , Pengfei Wang , Yuanchun Zhou

Single-cell RNA sequencing (scRNA-seq) enables researchers to analyze gene expression at single-cell level. One important task in scRNA-seq data analysis is unsupervised clustering, which helps identify distinct cell types, laying down the…

Genomics · Quantitative Biology 2023-12-29 Weikang Jiang , Jinxian Wang , Jihong Guan , Shuigeng Zhou

Clustering analysis is fundamental in single-cell RNA sequencing (scRNA-seq) data analysis for elucidating cellular heterogeneity and diversity. Recent graph-based scRNA-seq clustering methods, particularly graph neural networks (GNNs),…

Machine Learning · Computer Science 2025-07-15 Ping Xu , Pengfei Wang , Zhiyuan Ning , Meng Xiao , Min Wu , Yuanchun Zhou

Motivation: Single cell transcriptome sequencing (scRNA-Seq) has become a revolutionary tool to study cellular and molecular processes at single cell resolution. Among existing technologies, the recently developed droplet-based platform…

Machine Learning · Statistics 2017-04-10 Zhe Sun , Ting Wang , Ke Deng , Xiao-Feng Wang , Robert Lafyatis , Ying Ding , Ming Hu , Wei Chen

Since its selection as the method of the year in 2013, single-cell technologies have become mature enough to provide answers to complex research questions. With the growth of single-cell profiling technologies, there has also been a…

Single-cell RNA sequencing (scRNA-seq) provides high-dimensional profiles of cellular states, enabling data-driven modeling of cellular dynamics over time. In practice, time-resolved scRNA-seq is collected at only a few discrete time points…

Machine Learning · Computer Science 2026-05-22 Siyu Pu , Qingqing Long , Xiaohan Huang , Haotian Chen , Jiajia Wang , Meng Xiao , Xiao Luo , Hengshu Zhu , Yuanchun Zhou , Xuezhi Wang

Single-cell RNA-sequencing (scRNA-seq) stands as a powerful tool for deciphering cellular heterogeneity and exploring gene expression profiles at high resolution. However, its high cost renders it impractical for extensive sample cohorts…

Single-cell RNA sequencing (scRNA-seq) has made significant strides in unraveling the intricate cellular diversity within complex tissues. This is particularly critical in the brain, presenting a greater diversity of cell types than other…

Machine Learning · Computer Science 2023-10-05 Gyutaek Oh , Baekgyu Choi , Inkyung Jung , Jong Chul Ye

While single-cell RNA sequencing provides an understanding of the transcriptome of individual cells, its high sparsity, often termed dropout, hampers the capture of significant cell-cell relationships. Here, we propose scFP (single-cell…

Computational Engineering, Finance, and Science · Computer Science 2023-07-24 Sukwon Yun , Junseok Lee , Chanyoung Park

Cell type identification from single-cell transcriptomic data is a common goal of single-cell RNA sequencing (scRNAseq) data analysis. Neural networks have been employed to identify cell types from scRNAseq data with high performance.…

Genomics · Quantitative Biology 2020-05-11 Xishuang Dong , Shanta Chowdhury , Uboho Victor , Xiangfang Li , Lijun Qian

Computational modeling of single-cell gene expression is crucial for understanding cellular processes, but generating realistic expression profiles remains a major challenge. This difficulty arises from the count nature of gene expression…

Single-cell RNA sequencing (scRNA-seq) technology provides high-throughput gene expression data to study the cellular heterogeneity and dynamics of complex organisms. Graph neural networks (GNNs) have been widely used for automatic cell…

Machine Learning · Computer Science 2023-12-19 Rui Yang , Wenrui Dai , Chenglin Li , Junni Zou , Dapeng Wu , Hongkai Xiong

RNA inverse folding, designing sequences to form specific 3D structures, is critical for therapeutics, gene regulation, and synthetic biology. Current methods, focused on sequence recovery, struggle to address structural objectives like…

Machine Learning · Computer Science 2026-01-28 Qi Si , Xuyang Liu , Penglei Wang , Xin Guo , Yuan Qi , Yuan Cheng

Applications of single-cell RNA sequencing in various biomedical research areas have been blooming. This new technology provides unprecedented opportunities to study disease heterogeneity at the cellular level. However, unique…

Genomics · Quantitative Biology 2021-10-26 Xinlei Mi , William Bekerman , Peter A. Sims , Peter D. Canoll , Jianhua Hu

Single-cell RNA sequencing (scRNA-seq) is widely used to reveal heterogeneity in cells, which has given us insights into cell-cell communication, cell differentiation, and differential gene expression. However, analyzing scRNA-seq data is a…

Machine Learning · Computer Science 2023-06-27 Yuta Hozumi , Gu-Wei Wei

Single-cell RNA sequencing (scRNA-seq) enables the study of cellular diversity at single cell level. It provides a global view of cell-type specification during the onset of biological mechanisms such as developmental processes and human…

Machine Learning · Computer Science 2025-11-05 Muhammad Umar , Andras Lakatos , Muhammad Asif , Arif Mahmood

Single-cell RNA sequencing (scRNA-seq) is a fast growing approach to measure the genome-wide transcriptome of many individual cells in parallel, but results in noisy data with many dropout events. Existing methods to learn molecular…

Quantitative Methods · Quantitative Biology 2018-02-27 Beyrem Khalfaoui , Jean-Philippe Vert

Generating high-fidelity and biologically plausible synthetic single-cell RNA sequencing (scRNA-seq) data, especially with conditional control, is challenging due to its high dimensionality, sparsity, and complex biological variations.…

Machine Learning · Computer Science 2025-06-17 Lorenzo Bini , Stephane Marchand-Maillet

Single-cell multi-view clustering enables the exploration of cellular heterogeneity within the same cell from different views. Despite the development of several multi-view clustering methods, two primary challenges persist. Firstly, most…

Genomics · Quantitative Biology 2023-11-30 Dayu Hu , Zhibin Dong , Ke Liang , Jun Wang , Siwei Wang , Xinwang Liu

Single cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for recovering gene expression data from an exponentially scalable number of individual cells or nuclei. However, sci-RNA-seq is a complex protocol that…