Related papers: Aberration correction in epi-fluorescence microsco…
We describe and experimentally validate an algorithm to reconstruct an unknown extended object from through-focus measured image intensities blurred by unknown aberrations. It is shown that the method can recover diffraction-limited image…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…
In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…
Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…
Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…
Aberrations limit optical systems in many situations, for example when imaging in biological tissue. Machine learning offers novel ways to improve imaging under such conditions by learning inverse models of aberrations. Learning requires…
Photographs taken with less-than-ideal exposure settings often display poor visual quality. Since the correction procedures vary significantly, it is difficult for a single neural network to handle all exposure problems. Moreover, the…
Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…
The structured illumination microscopy using unknown speckle patterns has shown the capacity to surpass the Abbe's diffraction barrier, giving the possibility to design cheap and versatile SIM devices. However, the state-of-the-art joint…
In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…
This work addresses image restoration tasks through the lens of inverse problems using unpaired datasets. In contrast to traditional approaches -- which typically assume full knowledge of the forward model or access to paired degraded and…
Fourier ptychographic microscopy is a computational imaging technique that provides quantitative phase information and high resolution over a large field-of-view. Although the technique presents numerous advantages over conventional…
A solution to the inversion problem of scattering would offer aberration-free diffraction-limited 3D images without the resolution and depth-of-field limitations of lens-based tomographic systems. Powerful algorithms are increasingly being…
A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…
We report a novel generalized optical measurement system and computational approach to determine and correct aberrations in optical systems. We developed a computational imaging method capable of reconstructing an optical system's…
Metalenses offer a path toward creating ultra-thin optical systems, but they inherently suffer from severe, spatially varying optical aberrations, especially chromatic aberration, which makes image reconstruction a significant challenge.…
The optics of any camera degrades the sharpness of photographs, which is a key visual quality criterion. This degradation is characterized by the point-spread function (PSF), which depends on the wavelengths of light and is variable across…
Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…
An optical imaging system forms an object image by recollecting light scattered by the object. However, intact optical information of the object delivered through the imaging system is deteriorated by imperfect optical elements and unwanted…