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Histopathological cancer diagnosis is based on visual examination of stained tissue slides. Hematoxylin and eosin (H\&E) is a standard stain routinely employed worldwide. It is easy to acquire and cost effective, but cells and tissue…

Computer Vision and Pattern Recognition · Computer Science 2022-07-12 Zhaoyang Xu , Xingru Huang , Carlos Fernández Moro , Béla Bozóky , Qianni Zhang

Digitized Histological diagnosis is in increasing demand. However, color variations due to various factors are imposing obstacles to the diagnosis process. The problem of stain color variations is a well-defined problem with many proposed…

Computer Vision and Pattern Recognition · Computer Science 2018-04-06 M Tarek Shaban , Christoph Baur , Nassir Navab , Shadi Albarqouni

Image-to-image translation (I2I) methods allow the generation of artificial images that share the content of the original image but have a different style. With the advances in Generative Adversarial Networks (GANs)-based methods, I2I…

Image and Video Processing · Electrical Eng. & Systems 2023-04-07 Igor Zingman , Sergio Frayle , Ivan Tankoyeu , Segrey Sukhanov , Fabian Heinemann

Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

Image and Video Processing · Electrical Eng. & Systems 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

In whole slide imaging, commonly used staining techniques based on hematoxylin and eosin (H&E) and immunohistochemistry (IHC) stains accentuate different aspects of the tissue landscape. In the case of detecting metastases, IHC provides a…

Image and Video Processing · Electrical Eng. & Systems 2022-08-30 Joseph Boyd , Irène Villa , Marie-Christine Mathieu , Eric Deutsch , Nikos Paragios , Maria Vakalopoulou , Stergios Christodoulidis

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Cycle-Consistent Adversarial Network (CycleGAN) is very promising in domain adaptation. In this report, an example in medical domain will be explained. We present struecture of a CycleGAN model for unpaired image-to-image translation from…

Computer Vision and Pattern Recognition · Computer Science 2026-02-09 Yanhua Zhao

In digital pathology, different staining procedures and scanners cause substantial color variations in whole-slide images (WSIs), especially across different laboratories. These color shifts result in a poor generalization of deep…

Image and Video Processing · Electrical Eng. & Systems 2021-07-27 Sophia J. Wagner , Nadieh Khalili , Raghav Sharma , Melanie Boxberg , Carsten Marr , Walter de Back , Tingying Peng

Computational histopathology image diagnosis becomes increasingly popular and important, where images are segmented or classified for disease diagnosis by computers. While pathologists do not struggle with color variations in slides,…

Image and Video Processing · Electrical Eng. & Systems 2020-07-27 Hanwen Liang , Konstantinos N. Plataniotis , Xingyu Li

We propose a virtual staining methodology based on Generative Adversarial Networks to map histopathology images of breast cancer tissue from H&E stain to PHH3 and vice versa. We use the resulting synthetic images to build Convolutional…

Image and Video Processing · Electrical Eng. & Systems 2020-03-18 Caner Mercan , Germonda Reijnen-Mooij , David Tellez Martin , Johannes Lotz , Nick Weiss , Marcel van Gerven , Francesco Ciompi

Hematoxylin and Eosin (H&E) staining is a widely used sample preparation procedure for enhancing the saturation of tissue sections and the contrast between nuclei and cytoplasm in histology images for medical diagnostics. However, various…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-28 Shikha Dubey , Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

The variation in histologic staining between different medical centers is one of the most profound challenges in the field of computer-aided diagnosis. The appearance disparity of pathological whole slide images causes algorithms to become…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Martin J. Hetz , Tabea-Clara Bucher , Titus J. Brinker

Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

Histopathological evaluation of tissue samples is a key practice in patient diagnosis and drug development, especially in oncology. Historically, Hematoxylin and Eosin (H&E) has been used by pathologists as a gold standard staining.…

Computer Vision and Pattern Recognition · Computer Science 2018-10-16 Amal Lahiani , Jacob Gildenblat , Irina Klaman , Shadi Albarqouni , Nassir Navab , Eldad Klaiman

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain…

Computer Vision and Pattern Recognition · Computer Science 2018-11-12 Edwin Yuan , Junkyo Suh

Generalization is one of the main challenges of computational pathology. Slide preparation heterogeneity and the diversity of scanners lead to poor model performance when used on data from medical centers not seen during training. In order…

Image and Video Processing · Electrical Eng. & Systems 2024-01-09 Nicolas Nerrienet , Rémy Peyret , Marie Sockeel , Stéphane Sockeel

Histopathological diagnoses of tumors in tissue biopsy after Hematoxylin and Eosin (H&E) staining is the gold standard for oncology care. H&E staining is slow and uses dyes, reagents and precious tissue samples that cannot be reused.…

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori
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